School of Engineering and Science - Jacobs University
School of Engineering and Science - Jacobs University
School of Engineering and Science - Jacobs University
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Material <strong>and</strong> Methods<br />
3.6 Plasmids<br />
Tab. 7. Plasmids used in these studies.<br />
Plasmid Relevant characteristics Reference/Source<br />
pGEM-TEasy cloning vector for T/A cloning, Amp r Promega<br />
pPVSA1<br />
pPVSA2<br />
1,4 kb PCR-product <strong>of</strong> pvsA (fragment1) in pGEM-T Easy,<br />
primer combination pvsA_fwd1_Spe <strong>and</strong> pvsA_rev6_Kpn ,<br />
template Pss22d, Amp r<br />
1,6 kb PCR-product <strong>of</strong> pvsA (fragment2) pGEM-T Easy, primer<br />
combination pvsA_fwd7_Kpn und pvsA_rev4_Bam , template<br />
Pss22d, Amp r<br />
This study<br />
This study<br />
pPVSA3 Spe I-Kpn I fragment <strong>of</strong> pPVSA1 in pPVSA2 This study<br />
pPVSA4<br />
pPVSA3 containing Km r -cassette (Kpn I-Fragment), suizidvector<br />
for pvsA-mutagenesis in Pss22d<br />
This study<br />
pACS1<br />
1.3 kb PCR fragment amplified by primer combination<br />
Achr1_fwd <strong>and</strong> Achr2_Kpn I_rev. in pGEM-T Easy, Amp r<br />
This study<br />
pACS2<br />
2 kb PCR fragment amplified by primer combination<br />
Achr5_EcoRV_fwd <strong>and</strong> Achr6_rev in pGEM-T Easy, Amp r<br />
This study<br />
pACS3 pACS1 with Km r resistance cassette, Amp r , Km r This study<br />
pACS4<br />
pACS3 insert in pACS2, suizide vector for mutagenesis <strong>of</strong> acsgenes<br />
in Pss22d, Amp r , Km r<br />
This study<br />
pBBR-MCS1 cloning vector, Cm r Kovach et al., 1994<br />
pBBR-11-Sid 6 kb Ps tI-fragment <strong>of</strong> Pss22d∆Sid in pBBR-MCS1, Cm r , Sp r This study<br />
pBBR-23-Sid 6 kb Ps tI-fragment <strong>of</strong> Pss22d∆Sid-23 in pBBR-MCS1, Cm r , Sp r This study<br />
pMKm vector containing kanamycin cassette, Km r Murillo et al., 1994<br />
pCAM-Not<br />
Sp r Amp r , carries the mTn5 SS40 transposon<br />
Wilson et al., 1995, H.<br />
Weingart<br />
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