School of Engineering and Science - Jacobs University

Material and Methods
PCR-Screening for achromobactin-like siderophore production:
iuc1-fwd:
TGCTGGACTGGTTCGATG
iuc1-rev:
GATCAGCAGGCAATAGG
iuc2-fwd:
AAATCGAGCAGACCCAGC
iuc2-rev:
TGTTGGTCATGCTCATCG
4 Methods
4.1 Bacterial growth conditions
4.1.1 Escherichia coli
E. coli cells were grown overnight at 37°C on LB agar plates containing the
appropriate antibiotics. Subsequently, E. coli cells were cultured overnight at
37°C by shaking at 250 rpm in LB medium containing the appropriate
antibiotics. 2 ml plastic reaction tubes and test tubes were used for growth of
small volumes of E. coli cultures (1-5 ml), and Erlenmeyer flasks were used for
growth of big volumes of E. coli cultures (50-500 ml).
4.1.2 Pseudomonas syringae
P. syringae cells were grown for 2-5 days at 28°C on MG agar plates
containing appropriate antibiotics. Subsequently, P. syringae cells were
inoculated in liquid media (KB, 5b or Pipes) and grown at 28°C by shaking at
280 rpm. Test tubes were used for growth of small volumes of P. syringae
cultures (5 ml) and Erlenmeyer flasks were used for growth of big volumes of
P. syringae cultures (20-250 ml).
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