School of Engineering and Science - Jacobs University

More documents

Recommendations

Info

Material and Methods 3.4.1 Complex medium for Escherichia coli LB-medium Lurea-Bertani-medium (Sambrook et al., 1989), pH 7.0 10 g Bacto-trypton 10 g NaCl 5 g Yeast extract Adjust to 1 L with H 2 O 3.4.2 Complex medium for Pseudomonas syringae KB-medium King’B medium (King et al., 1954), pH 7.2 20 g Peptone 1.5 g K 2 HPO 4 1.5 g MgSO 4 x 7 H 2 O 10 ml glycerol Adjust to 1 L with H 2 O 3.4.3 Minimal media for Pseudomonas syringae MG-medium Mannitol-Glutamate medium (Keane et al., 1970), pH 7.0 10 g Mannitol 2 g L-Glutamic acid 0.5 g KH 2 PO4 0.2 g NaCl 0.2 g MgSO 4 x 7 H 2 O Adjust to 1 L with H 2 O 21
Material and Methods Iron-free 5b medium Iron free 5b-medium (Bütter, 2003) buffer (ad 500 ml): 2.6 g KH 2 PO 4 5.5 g Na 2 HPO 4 2.5 g NH 4 Cl 1 g Na 2 SO 4 carbon source (ad 500 ml): 10 g Glucose 100 mg MgCl x 6 H 2 O 10 mg MnSO 4 x 4 H 2 O Buffer solution and carbon source are autoclaved separately. Iron-free Pipes medium Iron free Pipes medium (Schwyn & Neilands, 1987), pH 7.0 buffer (ad 500 ml): 30.24 g Pipes 0.3 g KH 2 PO4 1 g NH 4 Cl 1 g Na 2 SO 4 carbon source (ad 500 ml): 10 g Glucose 100 mg MgCl x 6 H 2 O 10 mg MnSO 4 x 4 H 2 O CAA medium Casamino-acids medium (Cornelis, Anjaiah et al., 1992) 5 g Difco Bacto casaminoacids 0.9 g K 2 HPO 4 x 3 H 2 O 0.25 g MgSO 4 x 7 H 2 O Adjust to 1 L with H 2 O 22
Page 1 and 2: Siderophore production of Pseudomon
Page 3 and 4: Acknowledgements This project was s
Page 5 and 6: Abbreviations Ach achromobactin-lik
Page 7 and 8: Contents 4.1.2 Pseudomonas syringae
Page 9 and 10: Contents 5.3 Influence of sideropho
Page 11 and 12: Introduction 2 Introduction Like hu
Page 13 and 14: Introduction In contrast to the rhi
Page 15 and 16: Introduction 2.1.2 Host, pathogen a
Page 17 and 18: Introduction The relationship betwe
Page 19 and 20: Introduction developement of resist
Page 21 and 22: Introduction Fe 2+ + H 2 O 2 → Fe
Page 23 and 24: Introduction 1997a; May et al., 199
Page 25 and 26: Introduction Pss22d demonstrated a
Page 27 and 28: Introduction 2.4 Aim of this study
Page 29: Material and Methods Tab. 4. Antibi
Page 33 and 34: Material and Methods Solution3, car
Page 35 and 36: Material and Methods 3.6 Plasmids T
Page 37 and 38: Material and Methods PCR-Screening
Page 39 and 40: Material and Methods on ice for 10
Page 41 and 42: Material and Methods stained in eth
Page 43 and 44: Material and Methods 4.2.5.3 Conver
Page 45 and 46: Material and Methods 4.2.5.9 Ligati
Page 47 and 48: Material and Methods strains were s
Page 49 and 50: Material and Methods 9.5). For sign
Page 51 and 52: Material and Methods gentle shaking
Page 53 and 54: Material and Methods columns were w
Page 55 and 56: Results 5 Results 5.1 Analysis of s
Page 57 and 58: Results The 13-kb open reading fram
Page 59 and 60: Results If integration is due to a
Page 61 and 62: Results Yersiniabactin is a siderop
Page 63 and 64: Results Next, a large number of tra
Page 65 and 66: Results Nevertheless the site of tr
Page 67 and 68: Results Achr2_KpnI_rev binding site
Page 69 and 70: Results Interestingly the mutant Ps
Page 71 and 72: Results XAD4-purification yielded i
Page 73 and 74: Results After changing the mobile p
Page 75 and 76: Results P P A Fig. 21. Isoelectic f
Page 77 and 78: Results reduced in comparison to th
Page 79 and 80: Results A-E represents single inocu
Page 81 and 82:
Results Tab. 10. Development of pop
Page 83 and 84:
Results Tab. 11. Distribution of th
Page 85 and 86:
Discussion bacteria with redundande
Page 87 and 88:
Discussion 6.2 Possible regulation
Page 89 and 90:
Discussion stationary phase transit
Page 91 and 92:
Discussion and bacterium (Loper et
Page 93 and 94:
Literature Bultreys, A., Gheysen, I
Page 95 and 96:
Literature Galperin, M. Y. (2006).
Page 97 and 98:
Literature Mazzola, M. (2002). Mech
Page 99 and 100:
Literature Schubert, S., Rakin, A.,
Page 101:
Literature Zou, J., Rodriguez-Zas,
show all

School of Engineering and Science - Jacobs University

Create successful ePaper yourself

Delete template?

Save as template?