School of Engineering and Science - Jacobs University

Material and Methods
Iron-free 5b medium
Iron free 5b-medium (Bütter, 2003)
buffer (ad 500 ml):
2.6 g KH 2 PO 4
5.5 g Na 2 HPO 4
2.5 g NH 4 Cl
1 g Na 2 SO 4
carbon source (ad 500 ml):
10 g Glucose
100 mg MgCl x 6 H 2 O
10 mg MnSO 4 x 4 H 2 O
Buffer solution and carbon source are autoclaved separately.
Iron-free Pipes medium
Iron free Pipes medium (Schwyn & Neilands, 1987), pH 7.0
buffer (ad 500 ml):
30.24 g Pipes
0.3 g KH 2 PO4
1 g NH 4 Cl
1 g Na 2 SO 4
carbon source (ad 500 ml):
10 g Glucose
100 mg MgCl x 6 H 2 O
10 mg MnSO 4 x 4 H 2 O
CAA medium
Casamino-acids medium (Cornelis, Anjaiah et al., 1992)
5 g Difco Bacto casaminoacids
0.9 g K 2 HPO 4 x 3 H 2 O
0.25 g MgSO 4 x 7 H 2 O
Adjust to 1 L with H 2 O
22