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Results colony PCR with cells of Sinorhizobium meliloti and Xanthomonas campestris. Both strains are known to contain similar siderophore synthetases but not the achromobactin biosynthetic gene cluster. While no PCR product was obtained for S. meliloti, a PCR fragment significantly smaller than the expected product was detected in X. campestris. A colony PCR of PsmDC3000 served as a negative control for each PCR mix, while cells of Pss22d and D. chrysanthemi serve as positive control. Figure 25 gives an example for a screening gel. In addition, the production of the achromobactin-like siderophore was investigated by IEF analysis, as already shown in Figure 21. Table 11 summarizes the preliminary results of both screening methods. So far, the presence of the achromobactin-like siderophore could be demonstrated for 19 different pathovars of P. syringae, indicating that the biosynthesis of the achromobactinlike siderophore is widespread among different pathovars of P. syringae. 73
Results Tab. 11. Distribution of the achromobactin-like siderophore among different pathovars of P. syringae. P. syringae strains were analyzed for their ability to produce the acromobactin-like siderophore (IEF analysis). Additionally the presence of the siderophore biosynthesis gene acsB was determined by PCR with the primer pair iuc2_fwd and iuc2_rev. pathovar strain Achromobactin-like PCR product PCR for IEF-band product iuc2 syringae Pss22d + + Pss22d∆Pvd + + Pss22d∆Sid - + Pss22d∆Ach - - B728a + + B301D + + B241 + + C72 + + aceris CFBP 2339 + + actinidiae MAFF 302091 + nd aptata GSPB 1080 + + atrofaciens GSPB 1440 + + atropurporea MAFF 301309 + (-) berberis CFBP 1727 + nd broussonetiae MAFF810036 + nd castaneae CFBP 4217 + nd coronafaciens DSM 50261 - - daphniphylli CFBP 4219 + nd delfinii ICMP 529 + nd glycinea Psg1a + + PG4180 + mori MAFF 301020 + + mosprunorum GSPB 1013 + (-) maculicola DC3000 - - papulans ICMP 4048 + + phaseolicola 1448A + + pisi GSPB 1477 + nd savastanoi GSPB 2259 + (-) tabaci GSPB 117 + + tomato 487 + (-) 74
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Siderophore production of Pseudomon
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Acknowledgements This project was s
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Abbreviations Ach achromobactin-lik
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Contents 4.1.2 Pseudomonas syringae
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Contents 5.3 Influence of sideropho
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Introduction 2 Introduction Like hu
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Introduction In contrast to the rhi
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Introduction 2.1.2 Host, pathogen a
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Introduction The relationship betwe
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Introduction developement of resist
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Introduction Fe 2+ + H 2 O 2 → Fe
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Introduction 1997a; May et al., 199
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Introduction Pss22d demonstrated a
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Introduction 2.4 Aim of this study
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Material and Methods Tab. 4. Antibi
Page 31 and 32: Material and Methods Iron-free 5b m
Page 33 and 34: Material and Methods Solution3, car
Page 35 and 36: Material and Methods 3.6 Plasmids T
Page 37 and 38: Material and Methods PCR-Screening
Page 39 and 40: Material and Methods on ice for 10
Page 41 and 42: Material and Methods stained in eth
Page 43 and 44: Material and Methods 4.2.5.3 Conver
Page 45 and 46: Material and Methods 4.2.5.9 Ligati
Page 47 and 48: Material and Methods strains were s
Page 49 and 50: Material and Methods 9.5). For sign
Page 51 and 52: Material and Methods gentle shaking
Page 53 and 54: Material and Methods columns were w
Page 55 and 56: Results 5 Results 5.1 Analysis of s
Page 57 and 58: Results The 13-kb open reading fram
Page 59 and 60: Results If integration is due to a
Page 61 and 62: Results Yersiniabactin is a siderop
Page 63 and 64: Results Next, a large number of tra
Page 65 and 66: Results Nevertheless the site of tr
Page 67 and 68: Results Achr2_KpnI_rev binding site
Page 69 and 70: Results Interestingly the mutant Ps
Page 71 and 72: Results XAD4-purification yielded i
Page 73 and 74: Results After changing the mobile p
Page 75 and 76: Results P P A Fig. 21. Isoelectic f
Page 77 and 78: Results reduced in comparison to th
Page 79 and 80: Results A-E represents single inocu
Page 81: Results Tab. 10. Development of pop
Page 85 and 86: Discussion bacteria with redundande
Page 87 and 88: Discussion 6.2 Possible regulation
Page 89 and 90: Discussion stationary phase transit
Page 91 and 92: Discussion and bacterium (Loper et
Page 93 and 94: Literature Bultreys, A., Gheysen, I
Page 95 and 96: Literature Galperin, M. Y. (2006).
Page 97 and 98: Literature Mazzola, M. (2002). Mech
Page 99 and 100: Literature Schubert, S., Rakin, A.,
Page 101: Literature Zou, J., Rodriguez-Zas,
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