School of Engineering and Science - Jacobs University

More documents

Recommendations

Info

Contents 4.2.6.2 Alkaline transfer of DNA............................................................. 39 4.2.6.3 Hybridisation and detection....................................................... 39 4.2.7 DNA-sequencing............................................................................ 40 4.3 Analysis of bacterial growth in vitro................................................... 40 4.4 Analysis of bacterial growth in planta................................................ 40 4.4.1 Wound inoculation......................................................................... 40 4.4.2 Spray inoculation........................................................................... 41 4.5 Analysis of siderophore-production................................................... 41 4.5.1 Uptake of 59 Fe................................................................................. 41 4.5.2 Determination of siderophore concentration by quantitative CAS-assay ...................................................................................... 42 4.5.3 Quantification of pyoverdin by fluorescence measurement...... 43 4.5.4 Purification of siderophores by XAD4 ......................................... 43 4.5.5 Isoelectric focussing of siderophores ......................................... 44 4.5.6 Gel-filtration ................................................................................... 44 4.5.7 Thin-layer chromatography .......................................................... 45 5 Results......................................................................................................... 46 5.1 Analysis of siderophores produced by Pss22d................................. 46 5.1.1 Construction of a pyoverdin-deficient mutant of Pss22d .......... 47 5.1.2 Southern blot analysis of Psg1a and Pss22d for the presence of yersiniabactin biosynthetic genes ............................................... 51 5.1.3 Screening for an additional siderophore of Pss22d by random mutagenesis................................................................................... 53 5.1.4 Construction of a marker exchange mutant of the achromobactin-like siderophore in Pss22d................................. 57 5.1.5 In vitro comparison of Pss22d and its siderophore mutants..... 59 5.1.6 Purification of the achromobactin-like siderophore for structure determination ................................................................................. 61 5.2. Comparison of siderophore production in Psg1a and Pss22d ....... 65
Contents 5.3 Influence of siderophore production on biocontrol activity and epiphytic fitness of Pss22d ................................................................ 69 5.4 Distribution of the achromobactin–like siderophore synthesis among plant associated P. syringae ................................................. 71 6 Discussion................................................................................................... 75 6.1 Identification of an achromobactin-like siderophore in P. syringae 75 6.2 Possible regulation of the achromobactin-like siderophore ............ 78 6.3 Influence of siderophore production on epiphytic fitness of Pss22d .............................................................................................................. 80 6.4 Distribution of the achromobactin-like siderophore among P. syringae pathovars......................................................................... 81 6.5 Outlook.................................................................................................. 82 Literature ........................................................................................................ 83
Page 1 and 2: Siderophore production of Pseudomon
Page 3 and 4: Acknowledgements This project was s
Page 5 and 6: Abbreviations Ach achromobactin-lik
Page 7: Contents 4.1.2 Pseudomonas syringae
Page 11 and 12: Introduction 2 Introduction Like hu
Page 13 and 14: Introduction In contrast to the rhi
Page 15 and 16: Introduction 2.1.2 Host, pathogen a
Page 17 and 18: Introduction The relationship betwe
Page 19 and 20: Introduction developement of resist
Page 21 and 22: Introduction Fe 2+ + H 2 O 2 → Fe
Page 23 and 24: Introduction 1997a; May et al., 199
Page 25 and 26: Introduction Pss22d demonstrated a
Page 27 and 28: Introduction 2.4 Aim of this study
Page 29 and 30: Material and Methods Tab. 4. Antibi
Page 31 and 32: Material and Methods Iron-free 5b m
Page 33 and 34: Material and Methods Solution3, car
Page 35 and 36: Material and Methods 3.6 Plasmids T
Page 37 and 38: Material and Methods PCR-Screening
Page 39 and 40: Material and Methods on ice for 10
Page 41 and 42: Material and Methods stained in eth
Page 43 and 44: Material and Methods 4.2.5.3 Conver
Page 45 and 46: Material and Methods 4.2.5.9 Ligati
Page 47 and 48: Material and Methods strains were s
Page 49 and 50: Material and Methods 9.5). For sign
Page 51 and 52: Material and Methods gentle shaking
Page 53 and 54: Material and Methods columns were w
Page 55 and 56: Results 5 Results 5.1 Analysis of s
Page 57 and 58: Results The 13-kb open reading fram
Page 59 and 60:
Results If integration is due to a
Page 61 and 62:
Results Yersiniabactin is a siderop
Page 63 and 64:
Results Next, a large number of tra
Page 65 and 66:
Results Nevertheless the site of tr
Page 67 and 68:
Results Achr2_KpnI_rev binding site
Page 69 and 70:
Results Interestingly the mutant Ps
Page 71 and 72:
Results XAD4-purification yielded i
Page 73 and 74:
Results After changing the mobile p
Page 75 and 76:
Results P P A Fig. 21. Isoelectic f
Page 77 and 78:
Results reduced in comparison to th
Page 79 and 80:
Results A-E represents single inocu
Page 81 and 82:
Results Tab. 10. Development of pop
Page 83 and 84:
Results Tab. 11. Distribution of th
Page 85 and 86:
Discussion bacteria with redundande
Page 87 and 88:
Discussion 6.2 Possible regulation
Page 89 and 90:
Discussion stationary phase transit
Page 91 and 92:
Discussion and bacterium (Loper et
Page 93 and 94:
Literature Bultreys, A., Gheysen, I
Page 95 and 96:
Literature Galperin, M. Y. (2006).
Page 97 and 98:
Literature Mazzola, M. (2002). Mech
Page 99 and 100:
Literature Schubert, S., Rakin, A.,
Page 101:
Literature Zou, J., Rodriguez-Zas,
show all

School of Engineering and Science - Jacobs University

Create successful ePaper yourself

Delete template?

Save as template?