biologia - Studia

CHARACTERIZATION OF ALPHA-AMYLASE FROM BACILLUS SUBTILIS STRAIN
Table 2.
Data obtained during the fermentation, based on which, the enzymatic activity and
the dry cell mass were calculated
Time
(h)
Adsorbance
at 600 nm
Dry cell
mass (mg)
Control
Absorbance at
565nm
Enzyme production
Absorbance of
sample at 565nm
Activity
(U/ml/min)
0 0.221 0.0233 0.725 0.715 0.0165
0.5 0.21 0.0222 0.735 0.729 0.0095
1 0.252 0.0266 0.714 0.707 0.0123
1.5 0.183 0.0193 0.718 0.651 0.112
2 0.227 0.024 0.702 0.682 0.0342
2.5 0.365 0.0386 0.712 0.703 0.0151
3 0.581 0.0614 0.72 0.703 0.0285
3.5 0.61 0.0644 0.702 0.664 0.0642
4 0.835 0.0882 0.699 0.510 0.324
4.5 0.79 0.0835 0.71 0.482 0.385
5 1.235 0.1305 0.715 0.380 0.563
5.5 1.452 0.1534 0.72 0.327 0.655
6 1.58 0.1669 0.716 0.346 0.62
6.5 1.828 0.1931 0.695 0.185 0.88
7 1.95 0.206 0.708 0.159 0.93
7.5 2.275 0.2403 0.721 0.252 0.78
8 2.105 0.2224 0.7 0.099 1.03
8.5 2.422 0.2558 0.698 0.047 1.12
9 2.125 0.2245 0.71 0.030 1.15
9.5 2.355 0.2488 0.695 0.046 1.12
10 2.42 0.2556 0.7 0.058 1.1
10.5 2.252 0.2379 0.703 0.158 0.93
11 2.158 0.228 0.695 0.145 0.95
11.5 1.852 0.1956 0.698 0.128 0.98
12 1.905 0.2012 0.71 0.089 1.05
12.5 1.937 0.2046 0.685 0.143 0.95
13 1.45 0.1532 0.698 0.134 0.97
13.5 1.35 0.1426 0.685 0.211 0.83
14 1.42 0.15 0.693 0.144 0.95
The specific product forming rate and the efficiency coefficient can be
calculated and these offer information on the efficiency of the fermentation process.
Thus, at the end of the fermentation, based on the obtained data, it resulted that:
q p = 1/0.15 · (0.95-0.0165) / 14 · 60, so q p =7.4 U enzyme/g biomass/min
The efficiency coefficient was calculated using the same data, and we obtained
Y p/x = (0.95-0.0165) / (0.15-0.233), so Yp/x=7370 U enzyme/g biomass.
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