biologia - Studia

More documents

Recommendations

Info

CHARACTERIZATION OF ALPHA-AMYLASE FROM BACILLUS SUBTILIS STRAIN Enzymatic activity of the raw enzymatic preparation and of the purified enzyme preparation Table 3. Temperature ( O C) Raw extract OD Control OD Sample Activity OD Control Purified preparation OD Sample Activity 20 0.56 0.215 0.7392857 0.385 0.178 0.645195 30 0.565 0.16 0.860177 0.379 0.16 0.693404 40 0.558 0.11 0.9634409 0.382 0.132 0.78534 50 0.56 0.122 0.9385714 0.372 0.138 0.754839 60 0.58 0.132 0.9268966 0.378 0.156 0.704762 Enzymatic activity (U/ml/min) raw purified Temperature ( O C) Fig. 3. Evolution of the alpha-amylase activity. This phenomenon can be explained by an incomplete precipitation of the alpha-amylase in the raw extract and on the other hand by the presence of starch rests in the raw extract, which influenced the experiment. Analysing the figure, we observe also that the enzymatic activity of the of the raw extract seems to remain approximatively at the same value, between 40 and 60 0 C. 127
M.. DRĂGAN-BULARDA,. R. CARPA, M. MIŞCA, V.-CĂLEAN 4. Determining the enzymatic kinetics There was determined the Michaelis-Menten constant and V max of reaction. The data used for calculating K m and V max are indicated in Table 4. No. [S] (mg/ ml) Calculating K m and V max in the case of Bac.subtilis alpha-amylase L/[S] (ml/ mg) Adsorbance Concentration Initial Final Initial Final V (mg/s) Table 4. L/V (s/mg) 1 0.2 5 0.023 0.021 0.214 0.188 0.000433 2307.692 2 0.4 2.5 0.044 0.039 0.398 0.356 0.0007 1428.571 3 0.6 1.66 0.066 0.058 0.599 0.522 0.001283 779.2208 4 0.8 1.25 0.089 0.082 0.802 0.738 0.001067 937.5 5 1 1 0.106 0.092 0.955 0.832 0.00205 487.8049 Fig. 5. The calculation of K m and V max by Lineweaver-Burk method. The Fig.5 shows the calculation of K m and V max by the Lineweaver-Burk method. Thus, 1/V max = 210.9, so thati V max =0.0047 mg/s, respectively 0.284 mg/min, while K m /V max = 428 K m =428 x V max , so that K m = 2.011 mg/ml 128
Page 1 and 2:
BIOLOGIA 1/2010
Page 3 and 4:
Ş.-C. MIRESCU, L. CIOBANU, V. ANDR
Page 5 and 6:
L. PÂRVULESCU similarities and dis
Page 7 and 8:
L. PÂRVULESCU The crayfish were id
Page 9 and 10:
L. PÂRVULESCU matrix for the A. to
Page 11 and 12:
L. PÂRVULESCU 1,80 F(2, 55)=0.4275
Page 13 and 14:
L. PÂRVULESCU 2,7 F(1, 32)=4.3007,
Page 15 and 16:
L. PÂRVULESCU between Caraş and N
Page 18 and 19:
STUDIA UNIVERSITATIS BABEŞ - BOLYA
Page 20 and 21:
STRUCTURE AND DYNAMICS OF THE PREDA
Page 22 and 23:
Page 24 and 25:
Page 26 and 27:
Page 28 and 29:
Page 30 and 31:
Page 32 and 33:
Page 34 and 35:
LEAF-BEETLES FROM THE AREA BISTRIł
Page 36 and 37:
Page 38 and 39:
Page 40 and 41:
Page 42 and 43:
Page 44 and 45:
Page 46 and 47:
Page 48 and 49:
PLATFORM INITIATION, CLUTCH INITIAT
Page 50 and 51:
Page 52 and 53:
Page 54 and 55:
Page 56 and 57:
Page 58 and 59:
Page 60:
Page 63 and 64:
M. R. MOLAEI the infection and thei
Page 65 and 66:
M. R. MOLAEI . ⎧ ⎪ S( t) = bx(
Page 68 and 69:
Page 70 and 71:
Atm PROTEIN CAN SWITCH OFF THE DNA
Page 72 and 73:
Page 74 and 75:
Page 76 and 77:
Page 78 and 79: Atm PROTEIN CAN SWITCH OFF THE DNA
Page 80: Atm PROTEIN CAN SWITCH OFF THE DNA
Page 83 and 84: V. BERCEA, C. COMAN, N. DRAGOŞ In
Page 85 and 86: V. BERCEA, C. COMAN, N. DRAGOŞ (Fi
Page 87 and 88: V. BERCEA, C. COMAN, N. DRAGOŞ 0.8
Page 89 and 90: V. BERCEA, C. COMAN, N. DRAGOŞ The
Page 91 and 92: V. BERCEA, C. COMAN, N. DRAGOŞ Heb
Page 93 and 94: R. TOROK OANCE, V. NICULESCU, M.N.
Page 104 and 105: STUDIA UNIVERSITATIS BABEŞ - BOLYA
Page 106 and 107: DIAGNOSIS BY ELECTRICAL RESISTANCE
Page 114 and 115: OXIDATIVE STRESS AND INFLAMMATION-K
Page 122 and 123: CHARACTERIZATION OF ALPHA-AMYLASE F
Page 134 and 135: MICROBIOLOGICAL AND ENZYMOLOGICAL S
show all

biologia - Studia

Create successful ePaper yourself

Delete template?

Save as template?