Jahresbericht 2005 - IPHT Jena

72
from cancer patients. The device combines techniques
of segmented flow with thermal management
of sample streams, according the given molecular
biological protocol and real-time optical
readout of the amplification process. The PCR
reaction unit was developed as all-glass microchannel
chip module, which is based on a patented
flow-through thermocycler-chip. Thermal management
is realized by a micro system made heat
plate, providing thermal control and management
for up to four thermal zones with minimized dimensions
of the thermal gap between the heater
zones. The PCR sample droplets are cycled during
the flow over specific temperature zones (see figure
3F on the color page). Main advantage of this
LabOnChip module is the ability to process a high
number of individual samples, each embedded in
a single micro droplet by the serial flow regime.
Possible fields of application of the RT-PCR are
clinical diagnostics. For the establishment of the
system disseminated tumour cells in the blood of
patients with cervical carcinoma will be detected.
A special characteristic of these tumour cells is
the expression of oncogenes encoded by Human
Papillomaviruses (HPV), which can be specifically
amplified by this procedure.
A successful PCR reaction of HPV targets in
microchannels was demonstrated.
B. Chipmodules for analysis
of micro combustion
(G. Mayer, J. Albert, T. Henkel)
Chip modules for investigation of miniaturized
combustion processes have been developed and
successfully tested during an internal collaboration.
Integrated static micro-mixers allow the efficient
mixing of fuel gas and oxygen at macroscopic
explosive conditions and the controlled combustion
at the outlet nozzle. Up to 2300 K may be
generated in micro-flames with a width of 2 mm
and a height of 3 mm during micro combustion of
Fig. 3.15: All-glass chip with integrated static
mixer for analysis of micro flames.
MIKROSYSTEME / MICROSYSTEMS
oxygen and hydrogen. Detailed results on the
analysis of these micro chemical high temperature
processes are shown in the contribution of
the laser diagnostics department in this annual
report.
3.3 Appendix
Partners
National co-operation
• Alphacontec, Berlin
• Analytik AG, Jena
• Applikationszentrum Mikrotechnik, Jena
• ART-Photonics GmbH, Berlin
• Bartec Componenten und Systeme GmbH,
Gotteszell
• Berliner Glas KGaA Herbert Kubatz GmbH &
Co., Berlin
• Bosch und Siemens Hausgeräte GmbH,
Traunreuth
• Cetoni GmbH, Gera-Korbußen
• Deutsches Zentrum für Luft- und Raumfahrt
e.V., Berlin
• Entec GmbH, Ilmenau
• eta_max space GmbH, Braunschweig
• Fachhochschule Jena
• Fachhochschule Hannover, FB Elektrotechnik
• Fachhochschule Wiesbaden,
FB Physikalische Technik
• Faseroptik Jena GmbH, Bucha
• Fraunhofer Institut für Biomedizinische
Technik (IBMT) Nuthetal
• Friedrich-Schiller-Universität Jena
– Institut für Materialwissenschaft und Werkstofftechnologie
– Institut für Physikalische Chemie
– Institut für Physiologie II
– Klinik für Frauenheilkunde
• Fritz-Lipmann-Institut, Jena
• Hans-Knöll-Institut, Jena
• Hermsdorfer Institut für Technische Keramik
e.V., Hermsdorf
• HL-Planartechnik, Dortmund
• HSG, Institut für Mikrotechnik und Informationstechnik
e.V., Villingen-Schwenningen
• IL Metronic Sensortechnik GmbH, Ilmenau
• IMPAC Infrared GmbH, Frankfurt/Main
• Industrieanlagen-Betriebsgesellschaft mbH,
Ottobrunn
• Institut für Bioprozess- und Analysenmesstechnik
e.V. (iba), Heiligenstadt
• Institut für Mikrotechnik (IMM), Mainz
• Jena Bioscience GmbH, Jena
• JENOPTIK Laser, Optik, Systeme GmbH,
Jena
• JENOPTIK Mikrotechnik GmbH, Jena
• Kayser-Threde GmbH, München
• Labor Diagnostik Leipzig GmbH, Leipzig
• Max-Born-Institut, Berlin
• Micro-Hybrid Electronic GmbH, Hermsdorf