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LIST OF FIGURES
Figure I. I. Diagrammatic representation demonstrating the relationship between the
different symbols used in the formulae in Table 1.2. Reproduced from Priest and Austin
(1995) ................................................................................................
28
Figure 1.2. Representation of molecular taxonomy. Diagram outlining the molecular
technique of identifying relationships between species
.................................
29
Figure 2.1a A map of the Vestfold Hills, Eastern Antarctica, showing location of the 38
sampled lakes (reproduced with permission of the Australian Antarctic data centre) ....
34
Figure 2.1b A map of the Larsemann Hills, Eastern Antarctica, showing the location of
the 2 sampled lakes (reproduced with permission from the Australian Antarctic data
centre) ................................................................................................
35
Figure 2.1c A map of the epishelf lake, Beaver Lake, MacRobertson Land (modified
from Laybourn-Parry et al., 2001b) ............................................................
36
Figure 2.2. Diagrammatic representation of the ARDRA procedure
...............
44
Figure 2.3. Lane delineation image from 1D elite gel analysis software (Amersham
Pharmacia Biotech)
..............................................................................
47
Figure 2.4a Image showing how the DNA bands in one lane are established by
measuring pixel intensity .....................................................................
47
Figure 2.4b Image showing how individual bands can be seen more clearly by removing
the background pixel intensity using a rolling disc algorithm with a disc radius of 20 ... 47
Figure 2.5a Image showing automatic band detection software prior to artefact
Removal
.......................................................................................
48
Figure 2.5b Image showing bands detected after deletion of artefactual bands ...... 48
Figure 2.6a Image showing standardisation across the gel suing retardation factor, which
alleviated any distortion due to uneven running of warping of the gel ...............
48
Figure 2.6b Image showing molecular weights assigned to each of the detectable bands
within the three 100 bp ladders, from which a curve is computed to allows cross gel
comparison
.......................................................................................
49
Figure 2.7. Image showing band matching software (1 D elite gel analysis software
(Amersham Pharmacia Biotech)
............................................................
49
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