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distinct and active polypeptides without the secretory signal and prosequences. Vestfold Hills, East Antarctica. Antarctic Science. 11.2,175-192. GIBSON, J. A. E. and BURTON, H. R. (1996). Meromictic Antarctic lakes as recorders of climate change: the structures of Ace and Organic Lakes, Vestfold Hills. Antarctica. Papers and Proceedings of the Royal Society of Tasmania. 130.2.73- 78. GONG, Z., EWART, K. V., HU, Z., FLETCHER, G. L. and HEW, C. L. (1996). Skin antifreeze protein genes of the winter flounder, Pleuronectes americanus, encode The Journal of Biological Chemistry. 271,8,4106-4112. GONZALEZ, C. J., SANTOS, J. A., GARCIA-LOPEZ, M. L. and OTERO, A. (000). Psychrobacters and related bacteria in freshwater fish. Journal of Food Protection. 63,3,315-321. GORBUNOV, B., BAKLANOV, A., KAKUTKINA, N.. WINDSOR. H. L. and TOUMI. R. (2001). Ice nucleation on soot particles. Aerosol Sciences. 32,199-215. GORE, D. B., CREAGH, D. C., BURGESS, J. S., COLHOUN, E. A., SPATE. A. P. and BAIRD, A. S. (1996a). Composition, distribution and origin of surficial salts in the Vestfold Hills, East Antarctica. Antarctic Science. 8,1,73-84. GORE, D. B., PICKARD, J., BAIRD, A. S. and WEBB, J. A. (1996b). Glacial Crooked Lake, Vestfold Hills, East Antarctica. Polar Record. 32,180,19-24. GRAETHER, S. P. and JIA, Z. (2001). Modelling Pseudomonas syringae ice nucleation protein as a beta-helical protein. Biophysical Journal. 80,3,1169-1173. GRAHAM, L. A., LIOW, Y. -C., WALKER, V. K. and DAVIES, P. L. (1997). Hyperactive antifreeze protein from beetles. Nature. 388,727-728. GRANDUM, S., YABE, A., NAKAGOMI, K., TANAKA, M., TAKEMURA, F.. KOBAYASHI, Y. and FRIVIK, P. -E. (1999). Analysis of ice crystal growth for a crystal surface containing adsorbed antifreeze proteins. Journal of ('rvstal Growth. 205,382-390. GREY, J., LAYBOURN-PARRY. J., LEAKEY, R. J. G. and McMINN, A. (1997). Temporal patterns of protozooplankton abundance and their food in Ellis Fjord. Princess Elizabeth Land, Eastern Antarctica. Estuarine Coastal Shel I 'Science. 45. 17-25. 208
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Page 1 and 2:
COLD ADAPTATION STRATEGIES AND DIVE
Page 3 and 4:
, 71 "If you march your Winter Jour
Page 5 and 6:
1.6.3. Molecular typing techniques
Page 7 and 8:
3.2.1.2.3. DOC and chlorophyll a ..
Page 9 and 10:
5.3.2.5. The Sphingomonas group, is
Page 11 and 12:
LIST OF FIGURES Figure I. I. Diagra
Page 13 and 14:
Figure 3.17a Mean bacterial abundan
Page 15 and 16:
LIST OF TABLES Table 1.1. Types of
Page 17 and 18:
List of Abbreviations AFP AFGP AFLP
Page 19 and 20:
ACKNOWLEDGEMENTS This study was fun
Page 21 and 22:
Chapter 1: INTRODUCTION 1.1 - Tempe
Page 23 and 24:
1999 ; Hand & Burton, 1981) or iden
Page 25 and 26:
Cold adaptation is dependant on the
Page 27 and 28:
not at 25°C is a function of the a
Page 29 and 30:
The most prolific literature on col
Page 31 and 32:
process known as thermal hysteresis
Page 33 and 34:
dominant, role in the ice-growth in
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explanation for this diversity is t
Page 37 and 38:
1.5.3.5 - Membrane bound solute tra
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een well established (Russell & Nic
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& Bowman, 2001; Labrenz & Hirsch, 2
Page 43 and 44:
The reaction mix contains both deox
Page 45 and 46:
gene mixtures and hence can be used
Page 47 and 48:
displaying a high number of common
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AACGTCGAAA AACCTCGAAA (Organism A)
Page 51 and 52:
particular set of environmental par
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Lake Maximum Depth (m) Approximate
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Larsemann Hills __ rp3rtrnent of th
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Sampling trips took two days during
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Sigma, Sigma-Aldrich Company Ltd.,
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oxidized and the sample then conver
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sample. An appropriate volume was l
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min to ensure complete extension of
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Figure 2.3 - Lane delineation image
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Figure 2.6b - Molecular weights are
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RNA 41F (5'-GCT CAG ATT GAA CGC TGG
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I min. The spin column was removed
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compiled using Seqman and any erron
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ecorded and related to the level of
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information regarding the cold adap
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50ppt to 186ppt. Thirteen were sali
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M N N 't M M \p \p 00 kf) M kf) bA
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as would normally be expected, as b
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1444 6ý L CC vý O Q O O NO u 'c z
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5 - 250 3 245 240 G (, -3 235 a 0-
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3.2.2.2.5 - Dissolved organic carbo
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d 14 12 10 14 12 7 10 8 6 4 2 0 00/
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SRP ('g L- 05 10 15 20 2 3 Depth4 5
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3.2.2.3 - Biological characteristic
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a 0 Bacterial Abundance (x 106 ml-1
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3.3 - Discussion 3.3.1 - Summer sam
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to high surface water temperatures
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chemocline (Rankin et al., 1999). M
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a 250 200 150 100 50 70 60 50 40 ý
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The temperature (Fig 3.2a) through
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et al., 1999). No melt out was obse
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increase in ammonium is probably du
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10 7 9 6 8 E ö 7 5 Co u c m c 40 w
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main pigments were chlorophylls a a
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Inorganic nitrogen (NO2, NO3 and NH
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744 -- 7 644 6 19 ü SE 400-- 4 2s
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salinity decreased from July to Sep
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a Bacterial abundance (x 106 1.1) 0
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8 ö a N Ö oý L _> Q O O 4 0 rn O
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undoubtedly true of Club Lake. The
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suggested that input of bacteria an
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Pendant Lake is an unstratified, sa
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non-AFP active peptides from inhibi
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. ter rý :. 'ý Figure 4.2 -Fryka
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-ý- ---- ,. r. __-_-. ___. r. ýý
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4.2 - Results 4.2.1 - Sampling and
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SPLAT score. It has been shown that
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3'E t ý _d M! .PJ r rn 15 3.5 4 3
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4.3 - Discussion Of the 866 bacteri
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arisen in very few species due to c
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Chapter 5: BACTERIAL MOLECULAR TYPI
Page 153 and 154:
Fig 5.1 - Photographs of ARDRA gel
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Coefficient: Dice Algorithm: lPGMAA
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The closest published relative for
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identification was made based on 15
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Isolate number Closest phylogenetic
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e included due to its small sequenc
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5.3.2 - Identification of the AFP a
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explains the fluctuation in related
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contamination was lower as the PCR
Page 171 and 172:
seen to a certain extent in the ARD
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American Type Culture Collection (A
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Phylogenetic cluster alignment of t
Page 177 and 178: et al., 1994) and the Antarctic SIM
Page 179 and 180: Chapter 6: BACTERIAL COMMUNITY ANAL
Page 181 and 182: 6.2.1 - Detection of AFP active iso
Page 183 and 184: using the same PCR protocol as orig
Page 185 and 186: Figure 6.1 - Denaturing gradient ge
Page 187 and 188: 6.2.2 - Temporal and spatial variat
Page 189 and 190: Figure 6.4c - Gel I image showing b
Page 191 and 192: Coefficient: Dice Algorithm: UPGMA
Page 193 and 194: etween Om and 2m was obviously the
Page 195 and 196: All PCR-based rRNA molecular techni
Page 197 and 198: 6.3.2.3 - Cellular lysis and DNA pu
Page 199 and 200: hybrids should be equal. However, S
Page 201 and 202: contain contaminants. If the profil
Page 203 and 204: (Section 6.3.2.4.3 ) could not be r
Page 205 and 206: most dominant species in the commun
Page 207 and 208: study was characterised as M protea
Page 209 and 210: 11 species) showed AFP activity. Th
Page 211 and 212: that AFP activity could have evolve
Page 213 and 214: 7.2 - Future Work The 19 AFP active
Page 215 and 216: REFERENCES ABYZOV, S. S. (1993). Mi
Page 217 and 218: BELL, E. M. and LAYBOURN-PARRY, J.
Page 219 and 220: Symposium on Environmental Biogeoch
Page 221 and 222: Research. 5,477-493. CLESCERI, L. S
Page 223 and 224: DRICKAMER, K. (1999). C-type lectin
Page 225 and 226: antifreeze proteins from Atlantic s
Page 227: FRANZMANN, P. D. and DOBSON, S. J.
Page 231 and 232: IVANOVA, E. P., KIPRIANOVA, E. A.,
Page 233 and 234: Antarctic Ecosystems. G. A. Llano (
Page 235 and 236: Howard-Williams and I. Hawes). Balk
Page 237 and 238: W. (1998). Rep-PCR-mediated genomic
Page 239 and 240: putida strains from Antarctica. Mic
Page 241 and 242: NICHOLS, D., BOWMAN, J., SANDERSON,
Page 243 and 244: PEARCE, D. A. and BUTLER, H. G. (20
Page 245 and 246: (1999). Palaeohydrological modellin
Page 247 and 248: tracers of biogeochemical processes
Page 249 and 250: R. G. Landes Company, Austin, Texas
Page 251 and 252: partial 16S rDNA sequencing. Applie
Page 253 and 254: YABUUCHI, E., WANG, L., ARAKAWA, M.
Page 255 and 256: APPENDICES Al. Media Tryptic Soya A
Page 257 and 258: "D C 'O I. .. r .. r 6 rr E , 4mo .
Page 259 and 260: . + + + I I I I I I + I + + I I I I
Page 261 and 262: Ö ON N M 1 O - N 'zt "o l'" 00 C N
Page 263 and 264: 1 T 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Page 265 and 266: 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1
Page 267 and 268: 1 1 1 1 I 1 + I I 1 I 1 1 I 1 1 1 I
Page 269 and 270: 1 1 1 1 1 1 1 I I 1 I 1 I 1 1 I T I
Page 271 and 272: Cl M 'tt tn '. p t- 00 O\ N M O - (
Page 273 and 274: d' O - (N M 'nt V) \O N. 00 O> N M
Page 275 and 276: 1 1 T 1 1 1 T 1 1 1 1 1 1 1 1 1 1 1
Page 277 and 278: + + i i i i i i " i " . + + + + " +
Page 279 and 280:
e O --ý N M t Vn 1,0 [-- 00 O> N M
Page 281 and 282:
FT ce + I I r. to \, O t- 00 O\ N M
Page 283 and 284:
kn 110 r- 00 Oý N M O - N M Itt vn
Page 285 and 286:
, It N M "t N M Iýt N M M M M Oý
Page 287 and 288:
gj a - - - - - - - - - - - - - - -
show all

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