ý.,,: V. ý ýý . - Nottingham eTheses - University of Nottingham

Figure 3.17a Mean bacterial abundance against mean ammonia concentrations for Ace
Lake
................................................................................................
95
Figure 3.17b Relationship between average bacterial abundance with depth and average
DOC concentration with depth over four sampling dates in Ace Lake
...............
95
Figure 3.18. Relationship between bacterial abundance, PNAN abundance and HNAN
abundance in Ace Lake
.....................................................................
97
Figure 3.19. Diagrammatic representation of the profile of Pendant Lake indicating the
anoxic sump
.......................................................................................
98
Figure 3.20. Relationship between bacterial, PNAN and HNAN abundances for Pendant
Lake over time as averages of depth (0-1 Om) ..................................................
101
Figure 3.22a, b&c
Correlation between bacterial abundance and chlorophyll a
concentration for three sampling dates in 2000, July (a), September (b) and November
(c), in Triple Lake.
.............................................................................
105
Figure 3.21. Ambient air temperature graph for Davis station, Vestfold Hills, Antarctica
(reproduced with the permission of the Bureau of Meteorology, Melbourne,
Australia)
......................................................................................
107
Figure 4.1. Ice recrystalisation at -6°C for 30 minutes in a (A) AFP solution of 30%
sucrose and I mg/ml Fish AFP III, and (B) non-AFP solution of 30% sucrose
(Reproduced from Mills, 1999)
...........................................................
116
Figure 4.2. Fryka KP 281 cold block (CamLab) in situ with HTAP microtitre plates
during the recrystalisation step at -6°C in cold room .........................................
117
Figure 4.3. Photographic image of a high-throughput AFP assay in a 96 well microtitre
plate. Demonstrating technique and sample colouration
................................
117
Figure 4.4. HTAP assay in a 96 well microtitre plate. Demonstrating lack of contrast 119
Figure 4.5. Percentages of `SPLAT' assay confirmed AFP active bacteria isolated from
each lake ......................................................................................
123
Figure 4.6. Total cellular protein extraction concentration (mg mUl) with SPLAT score
with error bars for AFP active isolates
..................................................
125
Figure 4.7. Photography of SPLAT analysis of isolate 494 ................................
125
Figure 5.1. Photographs of ARDRA gel patterns used for phenetic analysis of band
position along gel (a) Hpa II and (b) Alu I
..................................................
133
iii