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Figure 5.2. Dendrogram calculated using the UPGMA algorithm from the similarity
matrices of (a) Hpa II and (b) Alu I digest patterns
.........................................
135
Figure 5.3. Photograph of an over-exposed gel image (b) next to a normal exposure gel
image (A), indicating the low molecular weight bands of bands of low DNA
concentration which can be better identified when the image is over-exposed
..... 135
Figure 5.4. Maximum likelihood phylogenetic tree of relatedness for 13 AFP active
isolates aligned against 18 published close relatives .........................................
142
Figure 5.5. ARDRA analysis of gel of Hpa II restriction digest of (1) isolate 494. (2)
M3 C203B-B, (3) Marinomonas protea and (4) SIA 181-1 A1....................... 152
Figure 6.1. Denaturing gradient gel electrophoresis acrylamide gel showing 10 highly
AFP active Antarctic lake isolates against 3 community profiles from the lakes of their
isolation
......................................................................................
165
Figure 6.2. Denaturing gradient gel electrophoresis acrylamide gel showing 9
representatives of phena identified within the AFP active isolate grouping (except M
protea related species) against 4 community profiles from the lakes of their isolation
. 165
Figure 6.3a DGGE gel 1. Matching analysis by Rf values to assess for the presence of
pure culture AFP active isolates within community fingerprints .......................
166
Figure 6.3b DGGE gel 2. Matching analysis by Rf value to assess for the presence of
pure culture AFP active isolates within community fingerprints .......................
166
Figure 6.4a DGGE community profiles for Ace Lake and Pendant Lake
..............
168
Figure 6.4b DGGE community profiles for Pendant Lake, Triple Lake, Deep Lake and
Club Lake
......................................................................................
168
Figure 6.4c Gel 1 image (Fig. 6.4a) showing band matching of Rf values ..............
169
Figure 6.4d Gel 2 image (Fig. 6.4b) showing band matching of Rf values ..............
169
Figure 6.5a Similarity matrix for the community fingerprint patterns of DGGE analysis
gels from fig 6.4a and fig 6.4b
...........................................................
170
Figure 6.5b Dendrogram showing phenetic relatedness of DGGE community profiles
for Ace Lake, Pendant Lake, Triple Lake, Deep Lake and Club Lake ..............
171
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