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American Type Culture Collection (ATCC) (Duman & Olsen, 1993) and isolated from
fresh pork sausages (McLean et al., 1951) and therefore although the Antarctic strain of
P. urativorans has not been identified as AFP active, there is a possibility it could
demonstrate activity based on the apparent AFP activity of isolate 583.
The grouping of isolate 583 with the other taxon within the phylogenetic tree (Fig,
5.4) also resembled the grouping shown between the associated isolates using the
ARDRA analysis, especially the Alu I digestion (Fig 5.2b). This further substantiates the
identification of isolate 583 as belonging to the genus Psychrobacter. Examples of the
Psychrobacter genus have been isolated from such diverse environments as freshwater
fish and vacuum-packed beef in cold storage (Sakala et al., 2002; Gonzalez et al., 2000)
and deep sea trenches whose deep-ocean circulation currents link it to surface waters in
Antarctica (Maruyama et al., 2000). There have been several isolations of Psychrobacter
from different Antarctic marine and terrestrial environments (Cavanagh, 1996: Duilio et
al., 2001; Camardella et al., 2002) indicating that Psychrobacter is readily associated
with the Antarctic marine environment, which links this genus to the marine derived
lakes of the Vestfold Hills.
5.3.2.7 -
The Enterobacter agglomerans group, isolate 732.
Isolate 732 shows a 99.5% similarity with Enterobacter agglomerans
(AF348161), which is a member of the Enterobacteriaceae family. When aligned with
Escherichia coli (JO 1859), Pantoea agglomerans/Enterobacter agglomerans (syn.
AJ233423/AF348161)
and Citrobacter freundii (AF025365), 732 clustered in a single
taxon with E. agglomerans (AF348161) and P. agglomerans (AJ233423). and showed a
close relationship with E. coli and C. freundii (Fig 5.4). In the cluster alignment the
Enterobacteriaceae family phenon showed close relationship with the Pseudoalteromona. s
genus (Fig 5.4), the statistical estimate of the likelihood of branching for the
Pseudoalteromonas / Enterobacter branch is 93% which suggests this is rather robust
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