o_19ko2dt161ng2j4e1tgnoqv1s45a.pdf

LEE et al (2007)
Table 2. Mean seed weights, germination rates and seedling performance after two years of
potting of four mother trees of S. lumutensis. Value in parentheses is the standard deviation.
Tree No.
No. of
seeds
Germination test
Seedling performance
after two years
No. of Mean seed % seed Mean height Mean
seeds weight/mg germinated /cm dgh/mm
004 85 50 12.0 (3.1) 6 - -
B005 190 50 20.5 (2.9) 54 23 (9) 3.7 (1.3)
B026 100 50 17.8 (2.8) 22 38 (12) 5.3 (1.2)
B385 120 50 24.6 (3.7) 60 28 (10) 4.1 (0.8)
Table 3. Locus names, primer sequences, repeat motifs, annealing temperatures (T), numbers
of alleles observed (A) and allele size ranges of microsatellites sequenced from the CT-enriched
genomic library of S. lumutensis. Expected heterozygosity (H e
), polymorphic information content
(PIC) and probability of paternity exclusion (P e
). * indicate a significant departure from Hardy-
Weinberg equilibrium (P < 0.05).
Locus Primer sequence (5’ - 3’) Repeat T A Size H e
PIC P e
Slu 044a F: ACA AAA AGT GGA TGG TGA G (GA) 15
50 3 138-152 0.535* 0.409 0.218
R: TTG TAG TGT TGT CCA GTG TG
Slu 057 F: TTT GTG GTC CCC GCC TTC TG (CT) 12
50 3 109-113 0.525 0.459 0.273
R: ATC AGA CAA TCT TTT TGG AC
Slu 110 F: CAT CCT TAC CTT TGT CAC CC (GA) 21
50 5 216-222 0.649 0.567 0.368
R: TCA GGC TCC ATT CTT CTT TT
Slu 124 F: GCA AAA TAA TAC TCA ATG GG (CA) 12
50 9 130-161 0.759 0.713 0.544
R: TGT CAC ATG GGT AAT AAA CT
Slu 175 F: CAT CAT TAC AAT CAT CCA TC (GA) 15
50 2 217-223 0.294 0.246 0.123
R: CAC TTG CTT CGT CGT CTA CC
ranged from 0.123 (Slu175) to 0.544 (Slu124). A significant departure from Hardy-Weinberg
equilibrium was detected on Slu044a. Linkage disequilibrium was found between Slu044a
and Slu175.
The study revealed high levels of genetic diversity in S. lumutensis (Table 4). The allelic richness
ranged from 5.7 (Lumut) to 6.3 (Segari Melintang) whereas the gene diversity ranged from
0.609 (Sungai Pinang) to 0.673 (Segari Melintang). The study also showed high positive values
of fixation index (F is
> 0.1) in all populations, an indication of an excess of homozygotes. The
spatial distribution of alleles study showed significant spatial genetic structure in SEE, SAP
and BIG but not in POL (Fig. 4). The coefficient of population differentiation quantified using
R-statistics showed that most of the total genetic diversity was partitioned within population.
The proportion of genetic diversity distributed among populations was estimated as 0.058, thus
only 5.8% of the genetic variability was distributed among populations. The cluster analysis
among populations, however, formed three genetic clusters; Lumut/Teluk Muroh, Sungai Pinang/
Pangkor Selatan, with Segari Melintang being the outlier (Fig. 5).
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