o_19ko2dt161ng2j4e1tgnoqv1s45a.pdf

LEE et al (2007)
SM
BI
LU
TM
52
SP
PS
Fig. 5. Neighbor-joining (NJ) cluster analysis based on D A
distances (Nei et al. 1983) among
the Sungai Pinang (SP), Lumut (LU), Pangkor Selatan (PS), Segari Melintang (SM) and Teluk
Muroh (TM) populations. The bootstrap values (based on 1000 replications) were generated
by PowerMarker software (Liu & Muse 2005).
The summary results of mating system, paternity assignment and breeding unit parameters are
given in Table 5. Shorea lumutensis can be inferred to follow the mixed-mating model (mean
outcrossing rate = 63.4%), with B004 showing the lowest value of outcrossing rate (22.2%)
and B005 the highest (92.0%). The pollen flow is moderately extensive, in the range of 122.0
m (B004) to 220.3 m (B385) with the mean of 175.2 m, and this allowed us to postulate that
low energy insects might be the main pollinators for S. lumutensis. In comparison with the
germination study, mother trees with higher outcrossing rate and receiving pollen from many
distant paternal trees produced bigger seeds, and bigger seeds have a greater probability to
germinate and establish seedlings. The mean breeding unit size and area were estimated as 52
individuals and 11.8 ha, respectively. The minimum population size to maintain current levels
of genetic diversity (number of alleles) is shown in Fig. 6. The basic relationship between A t
with sample size was logarithmic. To maintain 95% of alleles, 270 individuals are required (in
the range of 200-310 individuals).
Table 5. The summary results of mating system, paternity assignment and breeding unit
parameters of four half-sib families of S. lumutensis in Sungai Pinang. Value in parentheses is
the standard deviation.
Mating system and paternity assignment
% of seed
Mean pollen
% of seed due received
flow
to outcrossing pollen outside
distance/m
plot
Breeding unit parameter
Tree No.
No. of
seeds
Size/individual
Area/ha
B004 38 22.2 11.1 122.0 (0.0) 70 16.0
B005 50 92.0 24.0 220.0 (120.2) 47 10.7
B026 44 61.4 13.6 138.4 (28.3) 45 10.3
B385 50 78.0 16.0 220.3 (78.5) 44 10.1
Mean 45.5 63.4 (15.1) 16.2 (2.8) 175.2 (26.2) 52 (6) 11.8 (1.4)
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