Abstracts - Association for Chemoreception Sciences

#P183 POSTER SESSION IV:
CHEMICAL SIGNALING AND BEHAVIOR;
ANIMAL BEHAVIOR/PSYCHOPHYSICS;
CHEMOSENSATION AND METABOLISM;
VOMERONSASAL AND CHEMICAL
COMMUNICATION
#P184 POSTER SESSION IV:
CHEMICAL SIGNALING AND BEHAVIOR;
ANIMAL BEHAVIOR/PSYCHOPHYSICS;
CHEMOSENSATION AND METABOLISM;
VOMERONSASAL AND CHEMICAL
COMMUNICATION
Combinatorial and genotype specific co-expression of the
major urinary proteins (MUPs) during mouse postnatal
development: from fundamental aspects of olfaction to
innovative prospects in biomedicine
Sergey N. Novikov 1 , Elena M. Fedorova 1,2 , Irina I. Ermakova 3 , Anatoly
A. Philimonenko 4 , Gennady A. Churakov 5 , Sergey V. Mylnikov 6
1
I.P. Pavlov Institute of Physiology, Russian Academy of Sciences
Saint Petersburg, Russia, 2 Institute of Experimental Medicine, Russian
Academy of Medical Sciences Saint Petersburg, Russia, 3 Institute of
Cytology, Russian Academy of Sciences Saint Petersburg, Russia,
4
Institute of Molecular Genetics, v.v.i., Academy of Sciences of the
Czech Republic Prague, Czech Republic, 5 Institute of Experimental
Pathology/Molecular Neurobiology (ZMBE), University of Muenster
Muenster, Germany, 6 Saint Petersburg State University, Department of
Genetics and Biotechnology Saint Petersburg, Russia
Major urinary proteins (MUPs) of the house mouse form a large
group of highly polymorphic acidic isoforms with molecular
masses of 18-20 kDa. MUPs are encoded by the Mup gene
cluster, which consists of about 35 genes and pseudogenes and
is mapped to chromosome 4. Nowadays MUPs are considered
as a key component of the mouse olfactory signature which
can provide all essential information about the individuality of
donors. There is also rapidly growing evidence that several MUP
isoforms are involved in the regulation of glucose metabolism
(Zhou, Rui, 2010) and can be used as sensitive biomarkers in
early diagnosis of experimental nephritis (Wenderfer et al., 2009)
and hepatocarcinogenesis (Ritorto, Borlak, 2011). These studies
open practically unexplored biomedicine avenue for using MUPs
as new protein markers which are very suitable for diagnostic
purposes. We examined ontogenetic profiles of MUPs expression
in male and female mice of CBA/LacY and C57BL/6JY
strains using electrophoresis in polyacrylamide gel (PAGE).
Quantitative evaluation of eight MUPs isoforms (A-H) revealed
that each genotype is characterized by specific combinations
and different proportions (ratios) of the same MUP fractions.
These sex and genotype specific ratios emerged in both sexes
very soon after weaning, remain quite constant in adults and
resemble «barcode». Our data suggest that the pattern of Mup
genes expression during mouse ontogenesis is regulated through
a very stable genetic program. We suppose that at the early
stage of illness this ontogenetic program is destroyed and the
expression pattern of several Mup genes will be changed. These
processes are reflected in the appearance of new protein profiles
with altered MUPs’ ratios and may correspond to epigenetically
changed expression of the Mup gene cluster. Acknowledgements:
Supported by Russian Foundation for Basic Research (projects
02-04-49273, 07-04-01762).
The Association of Taste with Adiposity in the Beaver
Dam Offspring Study
Mary E. Fischer 1 , Karen J. Cruickshanks 1,2 , Carla R. Schubert 1 ,
Guan-Hua Huang 3 , Barbara E.K. Klein 1 , Ronald Klein 1 ,
James S. Pankow 4 , Nathan Pankratz 5 , Alex Pinto 1
1
University of Wisconsin, Department of Ophthalmology & Visual
Sciences Madison, WI, USA, 2 University of Wisconsin, Department
of Population Health Sciences Madison, WI, USA, 3 National Chiao
Tung University, Institute of Statistics Hsinchu, Taiwan, 4 University
of Minnesota, Division of Epidemiology & Community Health
Minneapolis, MN, USA, 5 University of Minnesota, Department of
Laboratory Medicine & Pathology Minneapolis, MN, USA
Taste sensation may influence food choice and consumption
which in turn may play a role in the maintenance of health.
The objective of this study was to determine the relationship
between taste and changes in adiposity and related health
measures during a 5 year follow-up period in the Beaver Dam
Offspring Study. Whole mouth suprathreshold taste intensity was
measured for salt, sweet, sour, and bitter at baseline (2005-2008)
using filter paper disks and a general labeled magnitude scale.
Health outcomes measured at baseline and follow-up (2010-
2013) included body mass index (BMI), waist circumference,
systolic and diastolic blood pressure, total cholesterol, and
hemoglobin A1c (HbA1c). Cluster analysis was used to group
participants according to observed patterns of intensities of the 4
tastes. In preliminary analyses (n = 1681, mean age at baseline =
48.9 years, range = 22-84 years), there were associations between
patterns of taste intensities and 5-year changes in BMI, waist
circumference, and HbA1c level. With adjustment for age and
sex, the cluster with high intensities for all 4 tastes demonstrated
a significantly greater mean increase in BMI (+ 0.96 kg/m 2 )
and HbA1C (+ 0.37%) than the cluster with average intensities
for the 4 tastes (BMI: + 0.32 kg/m 2 ; HbA1c: + 0.21%). Similar
results were observed for waist circumference (high intensities
cluster: + 3.01 cm; average intensities cluster: + 1.87 cm). In
these preliminary analyses, oral sensation, characterized using
patterns of perceived intensities of suprathreshold tastes, was
found to be associated with 5-year changes in some adiposityrelated
health outcomes. Acknowledgements: The project
described was supported by R01AG021917 from the National
Institute on Aging, National Eye Institute, and National Institute
on Deafness and Other Communication Disorders. The content
is solely the responsibility of the authors and does not necessarily
reflect the official views of the National Institute on Aging or the
National Institutes of Health.
POSTER PRESENTATIONS
Abstracts are printed as submitted by the author(s).
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