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Pigment Reduction in Corn Gluten Meal and Its Effects on Muscle ...

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LIST OF FIGURESFigure 2. 1 - Molecular structure of some important carotenoids founds <str<strong>on</strong>g>in</str<strong>on</strong>g> nature...................... 29Figure 2. 2 - Schematic representati<strong>on</strong> of the corn wet mill<str<strong>on</strong>g>in</str<strong>on</strong>g>g process. ...................................... 30Figure 2. 3 - Schematic representati<strong>on</strong> of the dry corn mill<str<strong>on</strong>g>in</str<strong>on</strong>g>g process. ...................................... 31Figure 2. 4 - Schematic representati<strong>on</strong> of the mode of acti<strong>on</strong> of lipoxygenase dur<str<strong>on</strong>g>in</str<strong>on</strong>g>g lipidoxidati<strong>on</strong> (Taylor <str<strong>on</strong>g>and</str<strong>on</strong>g> Morris 1983). a) Penta-1,4-cis-diene system with<str<strong>on</strong>g>in</str<strong>on</strong>g> l<str<strong>on</strong>g>in</str<strong>on</strong>g>oleic acid, b)Intermediate peroxy radical resp<strong>on</strong>sible for carotenoid pigments co-oxidati<strong>on</strong>, c) hydroperoxide,the f<str<strong>on</strong>g>in</str<strong>on</strong>g>al product of the enzyme-catalyzed lipid oxidati<strong>on</strong>............................................................ 32Figure 3. 1 - Typical trend for spectrophotometric measurement of lipoxygenase activityobta<str<strong>on</strong>g>in</str<strong>on</strong>g>ed for WSFF <str<strong>on</strong>g>in</str<strong>on</strong>g> the present study. ...................................................................................... 69Figure 3. 2 - Bleach<str<strong>on</strong>g>in</str<strong>on</strong>g>g pattern of carotenoids observed over time (120 m<str<strong>on</strong>g>in</str<strong>on</strong>g>) for the optimumfactor comb<str<strong>on</strong>g>in</str<strong>on</strong>g>ati<strong>on</strong>, PS = 90 m<str<strong>on</strong>g>in</str<strong>on</strong>g>, RT = 15°C, BP = 600 ppm <str<strong>on</strong>g>and</str<strong>on</strong>g> SO = 5% <str<strong>on</strong>g>in</str<strong>on</strong>g>clusi<strong>on</strong>. .............. 70Figure 3. 3 - Resp<strong>on</strong>se surface plot show<str<strong>on</strong>g>in</str<strong>on</strong>g>g the effect of SO <str<strong>on</strong>g>and</str<strong>on</strong>g> BP <strong>on</strong> CGM carotenoidsbleach<str<strong>on</strong>g>in</str<strong>on</strong>g>g, keep<str<strong>on</strong>g>in</str<strong>on</strong>g>g PS fixed at 90 m<str<strong>on</strong>g>in</str<strong>on</strong>g> at three different RT: 20, 15 <str<strong>on</strong>g>and</str<strong>on</strong>g> 10°C for A, B <str<strong>on</strong>g>and</str<strong>on</strong>g> C,respectively. .................................................................................................................................. 71Figure 3. 4 - Growth curve of ra<str<strong>on</strong>g>in</str<strong>on</strong>g>bow trout fed the experimental diets for 12 weeks. D1 (Diet 1,C<strong>on</strong>trol), D2 (Diet 2, regular CGM), D3 (Diet 3, Bleached CGM. .............................................. 72Figure 4. 1 - Growth curves of ra<str<strong>on</strong>g>in</str<strong>on</strong>g>bow trout fed the experimental diets for 24 weeks. ............ 102Figure 4. 2 - <strong>Muscle</strong> pigment c<strong>on</strong>centrati<strong>on</strong> to graded levels of corn gluten meal <str<strong>on</strong>g>in</str<strong>on</strong>g> ra<str<strong>on</strong>g>in</str<strong>on</strong>g>bow troutfed the experimental diets for 24 weeks. Larger markers <strong>on</strong> each trend l<str<strong>on</strong>g>in</str<strong>on</strong>g>e represent resultsobta<str<strong>on</strong>g>in</str<strong>on</strong>g>ed by diets where <str<strong>on</strong>g>in</str<strong>on</strong>g>clusi<strong>on</strong> of corn gluten meal was at expense of soy prote<str<strong>on</strong>g>in</str<strong>on</strong>g> c<strong>on</strong>centrate<strong>on</strong>ly (0, 9 <str<strong>on</strong>g>and</str<strong>on</strong>g> 18% CGM <str<strong>on</strong>g>in</str<strong>on</strong>g>clusi<strong>on</strong>). .......................................................................................... 103Figure 4. 3 - <strong>Muscle</strong> colour attributes to graded levels of corn gluten meal <str<strong>on</strong>g>in</str<strong>on</strong>g> ra<str<strong>on</strong>g>in</str<strong>on</strong>g>bow trout fedthe experimental diets for 24 weeks. Larger markers <strong>on</strong> each trend l<str<strong>on</strong>g>in</str<strong>on</strong>g>e represent results obta<str<strong>on</strong>g>in</str<strong>on</strong>g>edby diets where <str<strong>on</strong>g>in</str<strong>on</strong>g>clusi<strong>on</strong> of corn gluten meal was at expense of soy prote<str<strong>on</strong>g>in</str<strong>on</strong>g> c<strong>on</strong>centrate <strong>on</strong>ly (0, 9<str<strong>on</strong>g>and</str<strong>on</strong>g> 18% CGM <str<strong>on</strong>g>in</str<strong>on</strong>g>clusi<strong>on</strong>). .......................................................................................................... 104xi

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