VAAM-Jahrestagung 2011 Karlsruhe, 3.â6. April 2011

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acteriocines, proteins involved in cell wall metabolism, cell division,pyrimidine metabolism and metabolism of amino acids. The two componentsignal transduction systems CiaRH, VicKRX, RelRP, comDE andSMU.1037/1038 were significantly affected by carolacton. While comDE isthe only positively regulated TCS, VicKRX shows the earliest response tocarolacton. Among the other differentially expressed genes many known tobe regulated by VicKRX were identified, implicating a central role of theVicKRX-system in the mode of action of carolacton.An analysis of the sensitivity of all 13 viable HK-mutants of S. mutans tocarolacton using Live/Dead Bacterial Viability staining showed that allmutants were susceptible. Recently analysed regulon of eukaryotic typeserine/threonine kinase pknB showed a strong overlap with the carolactonaffected genes. Furthermore pknB is predicted to modulate the activity of theVicKRX system and interferes with the acid resistance. A pknB mutant wasproved to be insensitive to carolacton using Live/Dead staining and Cfucounts,indicating pknB as the potential target.[1] Kunze, B. et al (2010): Damage of Streptococcus mutans biofilms by carolacton, a secondarymetabolite from the myxobacterium Sorangium cellulosum. BMC.Microbiol. 10:199.SRP033Extracellular proteolysis of A. fumigatus and eIF2αkinase signaling - is there a connection?A. Bergmann* 1 , A. Schedler 1 , C. Sasse 2 , S. Krappmann 11 Center for Infectious Diseases, University of Wuerzburg, Würzburg,Germany2 Institute for Molecular Infection Biology, Julius-Maximilians-University,Würzburg, GermanyAspergillus fumigatus is a ubiquitous mould colonizing soil anddecomposing organic matter. It produces small conidia that are distributedby the air and reach the alveoli of the lung when inhaled. Inimmunocompromised patients, this opportunistic pathogen can cause severalforms of disease, the most severe form is called Invasive Aspergillosis (IA).Several determinants contribute to pathogenicity of A. fumigatus, e.g. itsnutritional versatility and the ability to react on fast changing environmentalconditions. The prtT gene product is a global regulator of extracellularproteolytic activity in A. fumigatus and is therefore involved in degradationof polymeric proteinaceous substances of the surrounding environment. Thistranscription factor regulates expression of several secreted proteases (alp1,mep, pep1); however, it is not a virulence determinant of pulmonaryinvasive aspergillosis in leukopenic mice. Expression of PrtT appears to beregulated posttranscriptionally, also supported by a long 5’ leader region ofthe prtT transcript. Furthermore, A. fumigatus exhibits eIF2α-kinasesignaling to counteract environmental stress conditions as it expresses twofunctional kinases for this initiation factor of translation: CpcC, an integralcomponent of the so-called Cross-Pathway Control system of amino acidbiosynthesis, and IfkB (initiation factor kinase B) with as yet unknowncellular function. To further investigate any regulatory role of the prtT leaderregion, we generated 5’prtT::gfp-reporter strains in genetic deletionbackgrounds for either or both eIF2α kinases. CpcC and IfkB seem toinfluence translation of the reporter upon a shift from minimal medium toBSA as sole nitrogen source with the presence of either sensor kinase beingapparently sufficient for expression. These data imply a mechanism oftranslational regulation of PrtT expression via two redundant eIF2α kinases,which links extracellular proteolysis of A. fumigatus to this conservedregulatory cascade.A Pkc1-GFP fusion protein has been reported to relocate from the cytoplasmto mitochondria upon treatment of yeast cells with the oxidative stress agentfarnesol (Fairn et al. 2007: J. Biol. Chem., 282, 4868-4874). In order toverify these data, we also constructed a similar Pkc1-GFP fusion in ourlaboratory strain and used it in combination with a mCherry-fusion of themitochondrial marker succinate dehydrogenase. We found that Pkc1-GFPprimarily localizes to the yeast bud neck during cytokinesis and does notrelocalize to mitochondria upon treatment with farnesol, tea trea oil ordetergents like Tween20 or Tween40. However, the nonionic detergentNonidet P-40, which was used by the authors cited above as a solvent forfarnesol, led to an accumulation of Pkc1-GFP at mitochondrial structures,even in the absence of farnesol.We conclude that the reported effect ofoxidative stress on Pkc1 localization is an experimental artefact.Currently, we are investigating the involvement of Pkc1 in the regulation ofyeast cytokinesis.SRP035A proteomic signature library: Gene expression ofStaphylococcus aureus under various growth-restrictingconditionsS. Fuchs*, D. Zühlke, J. Pané-Farré, H. Kusch, C. Wolf, S. Reiß,L.T.N. Binh, M. Hecker, S. EngelmannInstitute for Microbiology, Ernst-Moritz-Arndt-University, Greifswald,GermanySince decades, gel-based proteomics has been used to get deeper insightsinto physiological processes of living cells. Now we present an interexperimentalcomparison of different protein synthesis pattern induced inStaphylococcus aureus COL by nine various stressors or growth limitingfactors as hydrogen peroxide, diamide, paraquat, nitric oxide, heat,puromycin, mupirocin, and oxygen limitation in the presence or absence ofnitrate. Based on a cytoplasmic reference map with 698 identified proteinspots (521 different proteins) common and specific features of the individualstress responses were analyzed. Whereas only the synthesis of UspA(SACOL1759) was generally induced in six out of the nine experiments,several other proteins showed stress-specific expression profiles. Forinstance, synthesis of Rex-controlled proteins (e.g. Ldh1, SrrAB) wasclearly induced only after oxygen limitation or nitrosative stress. Exposureto H 2O 2 led to a stepwise adaptation that affects mainly expression ofproteins involved in DNA repair and nucleotide metabolism. Furthermore,expression data of more than 70 so far uncharacterized proteins areavailable. This might provide initial indications of their possiblephysiological role. All data generated in this study are stored in a custommadeonline resource which might be helpful for the interpretation of new(e.g. in vivo derived) expression data in future.SRP034Yeast protein kinase C does not relocate to mitochondriaupon membrane stress: evidence for experimentalartefactsS. Meyer*Department of Genetics, University of Osnabrück, Osnabrück, GermanyProtein kinase C (Pkc1) of the Baker's yeast Saccharomyces cerevisiae is akey component of the cell wall integrity (CWI) signalling pathway, whichgoverns cell wall biosynthesis upon cell surface stress. This pathway isessential for survival under normal growth conditions (i.e. in the absence ofosmotic stabilization) and constitutes an ideal target for the development ofantifungal agents. In addition to its role in activating the central MAP kinasemodule of the CWI pathway, Pkc1 influences the dynamics of the actincytoskeleton and is involved in the secretory pathway.spektrum | Tagungsband <strong>2011</strong>
263Aboubi, R.PSP015Abraham, W.-R. EMP044EMP058EMP073Abrashev, R.SRP025Abu Laban, N.AMP017Adam, A.OTP046Adam, B.AMP041Adamek, M.MPP012Adnan, F.SRP014Adrian, L.AMP040EMV004EMP118Aehle, W.ISV32Afonin, S.PSP002Aguiluz, K.CBV002Aguirre, J.FBP026Akob, D. M.EMV017Aksoyoglu, M.OTP012Aktas, M.MPV002MPP008Al-Karablieh, N. EMP102Al-Nasser, B.GWP023Al-Salamah, A. GWP001Alawi, M.EMP124Albarran, C.SIP015Albaum, S.FGP009Albermann, S.FBV019Albers, S.-V.ARV003ARV005ARP003ARP007Albert, A.EMV018Albrecht, R.CBP039Algheryani, H. FMP003Algora, C.EMV004Alhapel, A.AMP031Ali, A.OTP002Ali, Y.FMP016Alisch, R.OTP037Altenbuchner, J. GWP014Altendorf, K.EMV002Amann, R.EMV002EMV003EMV014EMV019EMV029MDV005MDP013Amato, L.FMV005Anderl, F.ARP009Andersson, A. F. EMV012Andrade-Junior, D. R. MPP025Andrade, S. L. A. OTP012OTP021RGV004 SRV006Andre, M.GWP046Andreeßen, B. GWV011Anetzberger, C. RGP031Angelov, A.FGP005FGP008Angelova, M.FBP027SRP025Anneser, B.EMV011EMP021Antelmann, H. SRV005Antoni, V.FBP004Apelt-Glowik, B. EMP064Aranda, E.GWP035Archer, J. A. C. EMP023Arends, K.MPP041Arendt, W.MPP042Arnds, J.MDV005Arnold, T.MDP001Arnstadt, T.Arzi, L.Ashwin, P.Asiimwe, T.Aung, Y.-Y.Aurich, A.Averhoff, B.Avondet, M.-A.FBP042MDP022OTP029CBV013MDP026EMP114FBP034MPP061OTP036OTP004Bach, J.CBP017Backhaus, K.FBV007Bahl, H.GWP019SRP017SRP024Bain, J.ISV21Bajerski, F.EMP030Bakken, L.MDP016Bakker, E. P.EMP047Balcerek, M.GWP039GWP042GWP045Bambic, D.ISV12Banasiak, R.FBP040Banerji, S.MPV003Bannert, A.EMP045Barbe, V.EMP056Barbier, B.EMP031Barbisan, C.MPP038Barends, T. R. AMV006Barig, S.OTP037Barkova, K.FBP041Barkovits, K.RGP042Barth, G.FBP034Bartnicki-Garcia, S. CBV011CBP040CBP041Bashir, S.EMP063Basse, C.RGP003Bathe, F.CBP019Batschauer, A.SRV004Bauch, M.FGP007Bauer, A.MPP040Bauer, T.CBP024Bauhus, J.MDP022Baumann, Sa.FMP019Baumann, Se.CBV007FBV008Baumeister, W.ISV26Baumgart, M.SRP007Baumgärtner, L. SRP029Baur, S.MPP044MPP049Bayer, A.EMV011EMP021Bayer, K.SIV008Bayer, P.ISV21Bayram, Z.FBP015RGV006Becher, D.FGP002SRV005SRV014Beck, Aa.EMV019Beck, Al.FGV001Beck, An.EMP114Becker, B.FBP007Becker, J.GWV006GWV013 SRV009Beheshti, M.OTP001Behr, S.RGP034Behrens, S.AMP035Beierkuhnlein, C. EMP003Beitzinger, C.Bender, J.Bender, T.Bengelsdorf, F.Bennati, M.Benndorf, D.Benninger, G.Bennke, C.Benz, R.Berditsch, M.Berendt, S.Berg, G.Berg, I.Berger, M.Berger, T. F.Berghoff, B. A.Bergh, I.Bergmann, A.Berks, B.Bernard, F.Berner, Z.Bernhardt, J.Berscheid, A.Berthold, T.Bertilsson, S.Bertram, R.Bertsche, U.Bettenbrock, K.Beyer, J.Beyer, L.Beyersmann, P.Bialek, B.Biasi, C.Biedendieck, R.Biegel, E.Biener, R.Bierbaum, G.Bijtenhoorn, P.Bill, E.Billenkamp, F.Billerbeck, S.Binder, T.Binh, L. T. N.Birch, P. R. J.Bischoff, M.Bischoff, Y.Biswas, A.Biswas, R.Bizic-Ionescu, M.Blank, L. M.Blaser, M.Bleiholder, A.Blombach, B.Blättel, V.Boch, J.MPP015MPP016MPP027OTP013MDP021AMP038AMP028OTP048MDP013MPP015MPP016CBP009PSP002CBP042EMP072PSP029EMP119RGP006OTP004RGP012RGP014SRV002SRP016OTP026SRP033AMP044GWP040EMP007FGP002MPP039MPP055EMP070EMV012MPV007PSP012RGP033MPV004SBP004MDP005AMP010EMP119ARP001EMP104GWP012AMP033GWP011GWP036MPP032MPP039MPP053MPP055GWP032OTP039AMP038EMP029SRP016EMP082EMP027SRP035ISV21MPV007NTP013EMP007MPP034EMV021EMV029GWP053AMP032AMP039ARP009GWV009GWP020FMP013ISV15MPP066Bode, H. B.SIV003SIP009SIP012SIP020SRP021Bode, L. M.MDP005Boddey, J. A.ISV21Boenisch, M.FBV015Boetius, A.ISV02MDV005Bohn, E.MPP013Boiangiu, C. D. AMP033Boll, M.AMV004AMP002AMP022AMP029AMP030EMV023Bollschweiler, C. SRP010Bolte, K.CBV006Bombach, P.NTP003Bonas, U.ISV15MPP066Bonfante, P.ISV17Bongaerts, J.EMP036FGP007 GWP010RGP023Boniadian, M.FMP009Bormann, J.FBV005FBV020Borowski, C.SIV007SIP021Borrmann, E.MPP043Borst, K.EMP065Bosch, J.AMP003Boschi Bazan, S. FBP023Bosello, M.FGP004Bott, M.GWP031NTV005PSP005SBP002SRP007Boulay, F.MPP013Bozinovski, D.EMP013Braker, G.MDP016Brakhage, A. A. FBV012FBV022FBP015FBP020FBP035FBP036MPV011MPV018MPP002MPP005MPP010MPP023RGP037SRV012Bramkamp, M.CBV014CBP007CBP016CBP017Brandt, F.ISV26RGP021Brankatschk, R. EMV018Bratfisch, F.ARV008Braun, H.-P.FBV022Braun, S. D.EMV026Braun, V. CBP012 CBP039Braunschweig, J. AMP003Braus, G. H. FBP015 RGV006Breidenbach, B. MDP008BIOspektrum | Tagungsband <strong>2011</strong>
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3Vereinigung für Allgemeine und An
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8 GENERAL INFORMATIONGeneral Inform
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12 GENERAL INFORMATION · SPONSORS
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14 GENERAL INFORMATIONEinladung zur
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16 AUS DEN FACHGRUPPEN DER VAAMFach
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22 INSTITUTSPORTRAITMicrobiology in
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INSTITUTSPORTRAITGrundlagen der Mik
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26 CONFERENCE PROGRAMME | OVERVIEWT
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28 CONFERENCE PROGRAMMECONFERENCE P
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30 CONFERENCE PROGRAMMECONFERENCE P
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32 SPECIAL GROUPSACTIVITIES OF THE
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34 SPECIAL GROUPSACTIVITIES OF THE
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36 SHORT LECTURESMonday, April 4, 0
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38 SHORT LECTURESMonday, April 4, 1
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40 SHORT LECTURESTuesday, April 5,
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42 SHORT LECTURESWednesday, April 6
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ISV01The final meters to the tapH.-
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ISV11No abstract submitted!ISV12Mon
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ISV22Applying ecological principles
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ISV31Fatty acid synthesis in fungal
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AMV008Structure and function of the
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pathway determination in digesters
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nearly the same growth rate as the
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the corresponding cell extracts. Th
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AMP035Diversity and Distribution of
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The gene cluster in the genome of t
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ARV004Subcellular organization and
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[1] Kennelly, P. J. (2003): Biochem
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[3] Yuzenkova. Y. and N. Zenkin (20
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(TPM-1), a subunit of the Arp2/3 co
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in all directions, generating a sha
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localization of cell end markers [1
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By the use of their C-terminal doma
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possibility that the transcription
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Bacillus subtilis. BiFC experiments
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published software package ARCIMBOL
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EMV005Anaerobic oxidation of methan
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esistance exists as a continuum bet
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ease of use for each method are dis
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ecycles organic compounds might be
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EMP009Isotope fractionation of nitr
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fluxes via plant into rhizosphere a
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EMP025Fungi on Abies grandis woodM.
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nutraceutical, and sterile manufact
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the environment and to human health
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EMP049Identification and characteri
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EMP058Functional diversity of micro
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EMP066Nutritional physiology of Sar
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acids, indicating that pyruvate is
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[1]. Interestingly, the locus locat
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mobilized via leaching processes dr
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Results: The change from heterotrop
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favorable environment for degrading
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for several years. Thus, microbiall
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species of marine macroalgae of the
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FBV003Molecular and chemical charac
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interaction leads to the specific a
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There are several polyketide syntha
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[2] Steffen, W. et al. (2010): Orga
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three F-box proteins Fbx15, Fbx23 a
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orange juice industry and its utili
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FBP035Activation of a silent second
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lignocellulose and the secretion of
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about 600 S. aureus proteins from 3
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FGP011Functional genome analysis of
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FMV001Influence of osmotic and pH s
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microbiological growth inhibition t
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Results: Out of 210 samples of raw
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FMP017Prevalence and pathogenicity
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hyperthermophilic D-arabitol dehydr
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GWV012Autotrophic Production of Sta
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EPS matrix showed that it consists
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enzyme was purified via metal ion a
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GWP016O-demethylenation catalyzed b
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[2] Mohebali, G. & A. S. Ball (2008
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finally aim at the inactivation of
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Results: 4 of 9 parent strains were
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GWP047Production of microbial biosu
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Based on these foregoing works we h
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function, activity, influence on gl
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selected phyllosphere bacteria was
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groups. Multiple isolates were avai
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Dinoroseobacter shibae for our knoc
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Here, we present a comparative prot
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MPV009Connecting cell cycle to path
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MPV018Functional characterisation o
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dependent polar flagellum. The torq
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(ciprofloxacin, gentamicin, sulfame
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MPP023GliT a novel thiol oxidase -
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that can confer cell wall attachmen
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MPP040Influence of increases soil t
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[4] Yue, D. et al (2008): Fluoresce
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hemagglutinates sheep erythrocytes.
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about 600 bacterial proteins from o
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NTP003Resolution of natural microbi
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an un-inoculated reference cell, pr
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NTP019Identification and metabolic
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OTV008Structural analysis of the po
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and at least 99.5% of their respect
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[2] Garcillan-Barcia, M. P. et al (
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Page 226 and 227: PSP022Genome analysis and heterolog
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Page 230 and 231: RGP002Bistability in myo-inositol u
Page 232 and 233: contains 6 genome copies in early e
Page 234 and 235: [3] Roppelt, V., Hobel, C., Albers,
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Page 238 and 239: RGP035Kinase-Phosphatase Switch of
Page 240 and 241: RGP043Influence of Temperature on e
Page 242 and 243: [3] was investigated. The specific
Page 244 and 245: transcriptionally induced in respon
Page 246 and 247: during development of the symbiotic
Page 248 and 249: [2] Li, J. et al (1995): J. Nat. Pr
Page 250 and 251: Such a prodrug-activation mechanism
Page 252 and 253: cations. Besides the catalase depen
Page 254 and 255: Based on the recently solved 3D-str
Page 256 and 257: [2] Wennerhold, J. et al (2005): Th
Page 258 and 259: SRP016Effect of the sRNA repeat RSs
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Page 264 and 265: 264 AUTORENBreinig, F.FBP010FBP023B
Page 266 and 267: 266 AUTORENGoerke, C.Goesmann, A.Go
Page 268 and 269: 268 AUTORENKlaus, T.Klebanoff, S. J
Page 270 and 271: 270 AUTORENMüller, Al.Müller, Ane
Page 272 and 273: 272 AUTORENScherlach, K.Scheunemann
Page 274 and 275: 274 AUTORENWagner, J.Wagner, N.Wahl
Page 276 and 277: 276 PERSONALIA AUS DER MIKROBIOLOGI
Page 278 and 279: 278 PROMOTIONEN 2010Lars Schreiber:
Page 280 and 281: 280 PROMOTIONEN 2010Universität Je
Page 282 and 283: 282 PROMOTIONEN 2010Universität Ro
Page 284: Die EINE, auf dieSie gewartet haben
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VAAM-Jahrestagung 2011 Karlsruhe, 3.â6. April 2011

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VAAM-Jahrestagung 2011 Karlsruhe, 3.â6. April 2011