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Fundamental Food Microbiology, Third Edition - Fuad Fathir

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254 FUNDAMENTAL FOOD MICROBIOLOGY<br />

3. Hugenholtz, J., Sybesma, W., Groot, M.N., Wisselink, W., Ladero, V., Burgess, K.,<br />

Van Sinderen, D., Piard, J-C., Eggink, G., Smid, E.J., Savoy, G., Sesma, F., Jansen,<br />

T., Hols, P., and Kleerebezem, H., Metabolic engineering of lactic acid bacteria for<br />

the production of nutraceuticals. Ant. van Leeuwen., 82, 217, 2002.<br />

4. Trivedi, N., Use of microorganisms in the production of unique ingredients, in<br />

Biotechnology in <strong>Food</strong> Processing, Harlander, S.K. and Labuza, T.P., Eds., Noyes<br />

Publications, Park Ridge, NJ, 1986, pp. 115-132.<br />

5. Jolly, L., Vincent, S.J.F., Duboc, P., and Neeser, J.-R., Exploiting exopolysaccharides<br />

from lactic acid bacteria, Ant. van Leeuwen., 82, 367, 2002.<br />

6. DeVuyst, L., DeVin, F., Vaningelgem, F., and Degeest, B., Recent developments in<br />

the biosynthesis and application of heteropolysaccharides from lactic acid bacteria.<br />

Int. Dairy J., 11, 687, 2001.<br />

7. Neidleman, S., Enzymology and food processing, in Biotechnology in <strong>Food</strong> Processing,<br />

Harlander, S.K. and Labuza, T. P., Eds., Noyes Publications, Park Ridge, NJ,<br />

1986, p. 37.<br />

8. While, T.J., Meade, J.H., Shoemaker, S.P., Koths, K.E., and Innis, M., Enzyme cloning<br />

for the food fermentation technology, <strong>Food</strong> Technol. 38(2), 90, 1984.<br />

9. Maugh, T.H., A renewed interest in immobilized enzymes, Science, 223, 474, 1984.<br />

10. Wasserman, B.P., Thermostable enzyme production, <strong>Food</strong> Technol., 38(2), 78, 1984.<br />

11. Shoemaker, S., The use of enzymes for waste management in the food industry, in<br />

Biotechnology in <strong>Food</strong> Processing, Harlander, S.K. and Labuza, T.P., Eds., Noyes<br />

Publications, Park Ridge, NJ, 1986, p. 259.<br />

QUESTIONS<br />

1. What are the advantages and disadvantages of using microbial proteins as human<br />

food?<br />

2. For each of the following, list one example from microbial origin that is used in<br />

food: amino acids, flavor compounds, flavor enhancers, color, polysaccharides<br />

(stabilizers), and nutraceuticals.<br />

3. Briefly indicate how some lactic acid bacteria can be engineered to produce large<br />

quantities of diacetyl and acetaldehyde (see Chapter 12).<br />

4. Name the microorganisms that are used to produce L(+)-lactic acid, acetic acid,<br />

and citric acid.<br />

5. List the advantages and disadvantages of using microbial enzymes and microbial<br />

cells in food processing.<br />

6. List five enzymes of microbial origin and discuss their specific uses in the food<br />

industry.<br />

7. Discuss the advantages of using immobilized enzymes in the production of food<br />

ingredients. List three methods of immobilization.<br />

8. What are the advantages of using a thermostable enzyme in the production of<br />

food ingredients (or a food)? How will recombinant DNA technology help in<br />

producing thermostable enzymes?<br />

9. Discuss how microbial enzymes can be helpful in the treatment of wastes from<br />

food production.<br />

10. “The potential of producing many types of food additives from food-grade microorganisms<br />

is very high.” Briefly comment on the possibilities (you may use<br />

materials from other chapters in the section).

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