GREEN SEED COAT COLOUR RETENTION IN LENTIL - University ...

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PERMISSION TO USE In presenting this thesis in partial fulfillment of the requirements for a Postgraduate degree from the University of Saskatchewan, I agree that the Libraries of this University may make it freely available for inspection. I further agree that permission for copying of thesis in any manner, in whole or in part, for scholarly purposes may be granted by the professor or professors who supervised my thesis work or, in their absence, by the Head of the Department or the Dean of the College in which my thesis work was done. It is understood that any copying or publication or use of this thesis or parts thereof for financial gain shall not be allowed without my written permission. It is also understood that due recognition shall be given to me and to the University of Saskatchewan in any scholarly use which may be of any material in my thesis. Requests for permission to copy or to make other use of material in this thesis in whole or in part should be addressed to: Head of the Department of Plant Sciences 51 Campus Drive University of Saskatchewan Saskatoon, Saskatchewan S7N 5A8 i
ABSTRACT Poor seed coat colour desirability in green lentil (Lens culinaris) costs lentil producers millions of dollars each year. The monetary value that Canadian lentil producers receive for their crop is based on the visual characteristics of the seed coat, mainly the colour. Higher value is given for samples described to have more desirable green seed coat colour. A breeding line, 1294M-23, has been noticed to consistently produce more desirable green lentil samples. A cross was made between 1294M-23 and a less desirable breeding line 1048-8R with the goal of studying the heritability of green seed coat desirability measured by the Acurum ® machine. The resulting progeny were taken to F7 by single seed descent. In 2005 and 2006 the recombinant inbred lines (RILs) were seeded in a randomized complete block design at three sites in the current main lentil growing region of Saskatchewan. To measure the seed coat colour of the samples, the Acurum ® machine, which is a colour analyzing machine developed for grain crops, was used to consistently compare the samples. The study illustrated that the trait has large environmental effects and is quantitative with a high broad sense heritability of 0.82, using this specific cross and environments. Transgressive segregation occurred for RILs that had more desirable green seed coats and lower index scores than the desirable parent, 1294M-23. A tester that included all registered green lentil cultivars set was grown with the RILs in all environments. The seed coat colour index scores of the tester set fit into a small section of the range of index scores. They all had relatively high mean index scores, meaning less ii
Page 1: GREEN SEED COAT COLOUR RETENTION IN
Page 5 and 6: ACKNOWLEDGEMENTS The author would l
Page 7 and 8: 7.2 Maturity and Index Score ......
Page 9 and 10: Table 4.13 Acurum index score, tota
Page 11 and 12: 1.0 INTRODUCTION Green lentil has b
Page 13 and 14: example canola. In wheat it is used
Page 15 and 16: carbohydrate, and micronutrient req
Page 17 and 18: It would also be useful for lentil
Page 19 and 20: process and categorize the data int
Page 21 and 22: Table 2.2 Descriptions of the colou
Page 23 and 24: 2.4 Perceived seed colour in green
Page 25 and 26: 2.6 Cotyledon chlorophyll degradati
Page 27 and 28: temperatures. The more enzyme activ
Page 29 and 30: the seed produces bleached seeds wi
Page 31 and 32: correlation between seed weight in
Page 33 and 34: used widely as a desiccant is that
Page 35 and 36: prevailing winds. A problem that oc
Page 37 and 38: and the higher the heritability est
Page 39 and 40: 4.1 Heritability Study Objectives 4
Page 41 and 42: Plant material for the study includ
Page 43 and 44: Table 4.4 Herbicide and desiccant a
Page 45 and 46: was a=0.05. The heritability was ca
Page 47 and 48: during the time taken to remove the
Page 49 and 50: normally distributed data. In 2006
Page 51 and 52: location and year explained a large
Page 53 and 54:
The tester set that was included in
Page 55 and 56:
etween field and phytotron index sc
Page 57 and 58:
Table 4.10 shows the mean index sco
Page 59 and 60:
Sutherland in 2005 produced the mos
Page 61 and 62:
Table 4.12 Effect of changing the n
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wrinkling as sound seeds. It is dif
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negative relationship. The regressi
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Chlorophyll b content (mg/g) 120 10
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1994). It is important to note howe
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estimates of which lines produced t
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in both years to produce two differ
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At the time of desiccation or swath
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a significant genotype x environmen
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Table 5.7 Mean green index score of
Page 81 and 82:
All three groups for both desiccati
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index score was not equal for each
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Alternatively, the samples had less
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sclerotinia white mold (Sclerotinia
Page 89 and 90:
6.0 GENERAL DISCUSSION Knowing whic
Page 91 and 92:
The Acurum ® machine has some pote
Page 93 and 94:
process. If small gains in desirabi
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for more efficient selection sooner
Page 97 and 98:
8.0 REFERENCES AAFC. 2007. Agricult
Page 99 and 100:
Guiamét, J.J., E. Schwartz, E. Pic
Page 101 and 102:
Shearer, S.A., G.M. Turner, M. Coll
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