GREEN SEED COAT COLOUR RETENTION IN LENTIL - University ...

More documents

Recommendations

Info

as parents in crossing blocks grown in the fall of 2003, summer of 2004, and winter of 2004. All of the seed was stored at room temperature in the Crop Science field laboratory from harvest until April 2007. Fifteen seeds were used as an experimental unit with no replication. 4.3 Results and Discussion 4.3.1 Heritability Study After the analysis was completed a labeling error for the plant material was discovered. The parents of the RIL population were screened using amplified fragment length polymorphism (AFLP) markers (Bett, 2007). The seed source used as the less desirable parent was mislabeled. Consequently the data from the less desirable green parent can not be compared to the RILs, only 1294M-23, the more desirable parent can be compared to the RILs. This means that conclusions about transgressive segregation past the least desirable parent can not be made. The material grown as the less green parent 1048-8R was still included in the tester set. It is unknown what the true line number is for the material labeled 1048-8R. Figure 4.1 depicts the distribution of the mean index scores for the RILs for all sites and years. The mean index score for all of the RILs for 2005 and 2006 at the three sites was 5.72 with a standard deviation of 1.41. This standard deviation was higher than the individual year × location standard deviations. The histogram was skewed to the right, but the data follow a normal distribution according to the Shapiro-Wilk test for normality. The RIL seed coat colour data followed a normal distribution when combined for all sites and years. In 2005 none of the sites had 38
normally distributed data. In 2006 all of the sites had data that fit the normal distribution when using the Shapiro-Wilk test for normality. Elrose in 2006 had the most desired green mean index score while Elrose in 2005 had the least desired mean green index score (Table 4.6). In 2005 Sutherland had the most desired green mean index score. In 2006 SPG had the least desired mean green index score. The smallest range in phenotype scores was Elrose in 2006 at 3.81. Elrose in 2005 had the largest range in phenotype scores at 6.60. The range in standard deviations of mean index score was 0.73 for Sutherland in 2005 to 1.48 for Elrose in 2005. This could mean that less weathering occurred on the lentil samples in the field compared with sites with a larger standard deviation. All sites in both years had significant differences among the genotypes evaluated. Table 4.6 Mean, range, test for normality, and dispersion statistic for the RILs that were grown at Elrose, SPG, and Sutherland during 2005 and 2006 for the heritability study of green seed coat colour differences in lentil. 2005 2006 All Sites Elrose SPG Sutherland Elrose SPG Sutherland Mean 6.86 6.45 5.03 4.31 5.90 5.20 5.72 Minimum 3.52 3.70 3.17 2.40 3.29 2.63 2.40 Maximum 10.44 10.30 8.58 6.21 8.85 8.65 10.44 LSD (0.05) 0.16 0.11 0.08 0.08 0.11 0.11 0.06 Standard deviation 1.48 1.05 0.73 0.73 1.05 1.05 1.41 Shapiro-Wilk statistic 0.99 0.98 0.98 0.99** 0.99** 0.99** 0.99** ** Denotes significant at a=0.05 Differences in green seed coat colour in lentil appear to be a quantitatively inherited trait with some environmental effects. There are no discrete index categories but rather a continuous range of possible desirability scores. Depending upon the site and year the size of the range of index scores differs. As the number of samples measured increases the distribution became more normal. This is seen in 39
Page 1 and 2: GREEN SEED COAT COLOUR RETENTION IN
Page 3 and 4: ABSTRACT Poor seed coat colour desi
Page 5 and 6: ACKNOWLEDGEMENTS The author would l
Page 7 and 8: 7.2 Maturity and Index Score ......
Page 9 and 10: Table 4.13 Acurum index score, tota
Page 11 and 12: 1.0 INTRODUCTION Green lentil has b
Page 13 and 14: example canola. In wheat it is used
Page 15 and 16: carbohydrate, and micronutrient req
Page 17 and 18: It would also be useful for lentil
Page 19 and 20: process and categorize the data int
Page 21 and 22: Table 2.2 Descriptions of the colou
Page 23 and 24: 2.4 Perceived seed colour in green
Page 25 and 26: 2.6 Cotyledon chlorophyll degradati
Page 27 and 28: temperatures. The more enzyme activ
Page 29 and 30: the seed produces bleached seeds wi
Page 31 and 32: correlation between seed weight in
Page 33 and 34: used widely as a desiccant is that
Page 35 and 36: prevailing winds. A problem that oc
Page 37 and 38: and the higher the heritability est
Page 39 and 40: 4.1 Heritability Study Objectives 4
Page 41 and 42: Plant material for the study includ
Page 43 and 44: Table 4.4 Herbicide and desiccant a
Page 45 and 46: was a=0.05. The heritability was ca
Page 47: during the time taken to remove the
Page 51 and 52: location and year explained a large
Page 53 and 54: The tester set that was included in
Page 55 and 56: etween field and phytotron index sc
Page 57 and 58: Table 4.10 shows the mean index sco
Page 59 and 60: Sutherland in 2005 produced the mos
Page 61 and 62: Table 4.12 Effect of changing the n
Page 63 and 64: wrinkling as sound seeds. It is dif
Page 65 and 66: negative relationship. The regressi
Page 67 and 68: Chlorophyll b content (mg/g) 120 10
Page 69 and 70: 1994). It is important to note howe
Page 71 and 72: estimates of which lines produced t
Page 73 and 74: in both years to produce two differ
Page 75 and 76: At the time of desiccation or swath
Page 77 and 78: a significant genotype x environmen
Page 79 and 80: Table 5.7 Mean green index score of
Page 81 and 82: All three groups for both desiccati
Page 83 and 84: index score was not equal for each
Page 85 and 86: Alternatively, the samples had less
Page 87 and 88: sclerotinia white mold (Sclerotinia
Page 89 and 90: 6.0 GENERAL DISCUSSION Knowing whic
Page 91 and 92: The Acurum ® machine has some pote
Page 93 and 94: process. If small gains in desirabi
Page 95 and 96: for more efficient selection sooner
Page 97 and 98: 8.0 REFERENCES AAFC. 2007. Agricult
Page 99 and 100:
Guiamét, J.J., E. Schwartz, E. Pic
Page 101 and 102:
Shearer, S.A., G.M. Turner, M. Coll
show all

GREEN SEED COAT COLOUR RETENTION IN LENTIL - University ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?