GREEN SEED COAT COLOUR RETENTION IN LENTIL - University ...

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important factor for seed coat darkening is time. As the time in storage increases the amount of seed coat darkening will increase. To prevent many complications selections for desirability in the field should be made soon after harvest. 4.4 Conclusion The heritability study proved that selection for improved green seed coat colour in lentil would be successful. There is currently little genetic variation in registered green lentil genotypes in Canada but some breeding material and breeding lines have much more variation for the Acurum ® index score. The heritability of the trait was relatively high even though it was a quantitatively expressed trait that had the environment producing some of the variation in phenotypes. To obtain an accurate estimate of the genotype effect from the phenotypic measurements several locations and years should be analyzed. This allows for estimates of interaction variances and raises the heritability estimate. If releasing green lentil lines with enhanced green seed coat colour desirability is a high priority, more sites, years, and replications should be used during testing. This will maximize the heritability and thus the response to selection assuming selection intensity and genetic variation are constant. Testing in the phytotron does not appear to work efficiently based on the results of this analysis even though the trait was first noticed in the phytotron. Phytotron index scores do not accurately rank the genotypes from most desired to least desired when comparing with field results. It is possible that without some deliberate treatment to reduce the green seed coat colour, that measurements from the phytotron were 60
estimates of which lines produced the most “green” seed coat at harvest, not the seed coat that withstood weathering the best. The amount of weathering that green lentil seed coats are exposed to increases their index score. In the phytotron no environmental or artificial weathering occurred to these samples and there index scores were different than the field index scores. The ranking of the genotypes grown in the phytotron would probably mimic field ranking if no weathering was allowed and harvest commenced individually for each genotype as it became ready for harvest. The ranking of most desired to least desired seed coat colour from the Acurum ® machine included field weathering. The amount of chlorophyll in the seed at harvest did not appear to be highly associated with the index score. When regression analysis was done on total chlorophyll, chlorophyll a, chlorophyll b, and chlorophyll a:b ratio and the index score, none produced strong relationships. 61
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GREEN SEED COAT COLOUR RETENTION IN
Page 3 and 4:
ABSTRACT Poor seed coat colour desi
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ACKNOWLEDGEMENTS The author would l
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7.2 Maturity and Index Score ......
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Table 4.13 Acurum index score, tota
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1.0 INTRODUCTION Green lentil has b
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example canola. In wheat it is used
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carbohydrate, and micronutrient req
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It would also be useful for lentil
Page 19 and 20: process and categorize the data int
Page 21 and 22: Table 2.2 Descriptions of the colou
Page 23 and 24: 2.4 Perceived seed colour in green
Page 25 and 26: 2.6 Cotyledon chlorophyll degradati
Page 27 and 28: temperatures. The more enzyme activ
Page 29 and 30: the seed produces bleached seeds wi
Page 31 and 32: correlation between seed weight in
Page 33 and 34: used widely as a desiccant is that
Page 35 and 36: prevailing winds. A problem that oc
Page 37 and 38: and the higher the heritability est
Page 39 and 40: 4.1 Heritability Study Objectives 4
Page 41 and 42: Plant material for the study includ
Page 43 and 44: Table 4.4 Herbicide and desiccant a
Page 45 and 46: was a=0.05. The heritability was ca
Page 47 and 48: during the time taken to remove the
Page 49 and 50: normally distributed data. In 2006
Page 51 and 52: location and year explained a large
Page 53 and 54: The tester set that was included in
Page 55 and 56: etween field and phytotron index sc
Page 57 and 58: Table 4.10 shows the mean index sco
Page 59 and 60: Sutherland in 2005 produced the mos
Page 61 and 62: Table 4.12 Effect of changing the n
Page 63 and 64: wrinkling as sound seeds. It is dif
Page 65 and 66: negative relationship. The regressi
Page 67 and 68: Chlorophyll b content (mg/g) 120 10
Page 69: 1994). It is important to note howe
Page 73 and 74: in both years to produce two differ
Page 75 and 76: At the time of desiccation or swath
Page 77 and 78: a significant genotype x environmen
Page 79 and 80: Table 5.7 Mean green index score of
Page 81 and 82: All three groups for both desiccati
Page 83 and 84: index score was not equal for each
Page 85 and 86: Alternatively, the samples had less
Page 87 and 88: sclerotinia white mold (Sclerotinia
Page 89 and 90: 6.0 GENERAL DISCUSSION Knowing whic
Page 91 and 92: The Acurum ® machine has some pote
Page 93 and 94: process. If small gains in desirabi
Page 95 and 96: for more efficient selection sooner
Page 97 and 98: 8.0 REFERENCES AAFC. 2007. Agricult
Page 99 and 100: Guiamét, J.J., E. Schwartz, E. Pic
Page 101 and 102: Shearer, S.A., G.M. Turner, M. Coll
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