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GREEN SEED COAT COLOUR RETENTION IN LENTIL - University ...

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was a=0.05. The heritability was calculated from the ANOVA of the RILs. The<br />

heritability estimate is broad sense as the RILs were not at complete homozygosity.<br />

They are approximately 99.2 percent homozygous at F7. The calculation of<br />

heritability is described by Falconer and MacKay (1996):<br />

H 2 = s 2 genetic / s 2 phenotypic , (4.2)<br />

H 2 = broad sense heritability,<br />

s 2 phenotypic = s 2 g + s 2 gl/l + s 2 gy/y + s 2 gly/ly + s 2 residual/rly,<br />

g = genotype,<br />

y = year,<br />

l = location and,<br />

r = block.<br />

4.2.2 Chlorophyll Extractions<br />

Chlorophyll extractions were performed on selected genotypes from a range<br />

of index scores and locations from the heritability study. Genotypes with an index<br />

score range of 2.40 to 8.62 were used (Table 4.5) to cover a large range from high<br />

quality green seed coats to those exhibiting bleaching and oxidation, in each sample<br />

used some seeds would have individually had lower index scores and some higher<br />

index scores. This range was used so that most of the seed could be sourced from<br />

one site.<br />

35

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