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GREEN SEED COAT COLOUR RETENTION IN LENTIL - University ...

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2005 when individual sites do not have a normal distribution but when all samples<br />

from all sites and years are combined the distribution is normal.<br />

Number of RILs<br />

35<br />

30<br />

25<br />

20<br />

15<br />

10<br />

5<br />

0<br />

2<br />

2.5<br />

3<br />

3.5<br />

4<br />

4.5<br />

5<br />

5.5<br />

6<br />

40<br />

6.5<br />

Index score<br />

Figure 4.1 Histogram of the mean index scores for the RILs used in the heritability<br />

study for green seed coat colour differences in lentil for 2005 and 2006 at<br />

Elrose, SPG, and Sutherland. Arrow denotes where the more desirable parent,<br />

1294M-23, would lie in the histogram.<br />

The data were subjected to an ANOVA using a mixed model. When the<br />

residual analysis was completed it showed a normal distribution of the residues<br />

when using the Shapiro-Wilk test for normality (a=0.05). The Bartlett test for<br />

homogeneity of the variances also showed that the inclusion of both years into the<br />

analysis was justified. This included all of the data from all three sites over the two<br />

year period. The ANOVA showed that the following effects were highly<br />

significant: location, year, location × year interaction, block, genotype, genotype ×<br />

location interaction, genotype × year interaction, and genotype × location × year<br />

interaction. Because genotype was significant some of the variability in index<br />

scores was explained by the genotype. As expected with most quantitative traits,<br />

7<br />

7.5<br />

8<br />

8.5<br />

9<br />

9.5<br />

10

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