GREEN SEED COAT COLOUR RETENTION IN LENTIL - University ...

More documents

Recommendations

Info

2005 when individual sites do not have a normal distribution but when all samples from all sites and years are combined the distribution is normal. Number of RILs 35 30 25 20 15 10 5 0 2 2.5 3 3.5 4 4.5 5 5.5 6 40 6.5 Index score Figure 4.1 Histogram of the mean index scores for the RILs used in the heritability study for green seed coat colour differences in lentil for 2005 and 2006 at Elrose, SPG, and Sutherland. Arrow denotes where the more desirable parent, 1294M-23, would lie in the histogram. The data were subjected to an ANOVA using a mixed model. When the residual analysis was completed it showed a normal distribution of the residues when using the Shapiro-Wilk test for normality (a=0.05). The Bartlett test for homogeneity of the variances also showed that the inclusion of both years into the analysis was justified. This included all of the data from all three sites over the two year period. The ANOVA showed that the following effects were highly significant: location, year, location × year interaction, block, genotype, genotype × location interaction, genotype × year interaction, and genotype × location × year interaction. Because genotype was significant some of the variability in index scores was explained by the genotype. As expected with most quantitative traits, 7 7.5 8 8.5 9 9.5 10
location and year explained a large part of the variability of index scores. This is because they explain environmental variation which is high for this trait. The estimate of genetic variance was 0.43 (Table 4.7). Phenotypic variance was 0.52. The phenotypic variance is based on the number of years, locations, and blocks used in the testing. The broad sense heritability was 0.82 (Appendix A). The estimate shows the heritability for differences in green seed coat colour are similar to the heritability of bleaching resistance in split green pea cotyledons which is estimated at 0.86 and a quantitative trait (Ubayasena, 2007). The high heritability of green seed coat colour differences among genotypes should ensure that future selection is effective for producing green lentil lines that have a more desirable green appearance. Table 4.7 Variance estimates of the sources of variation from the ANOVA of the heritability study of green seed coat colour differences in lentil with their standard error estimate. Parameter Variance Standard error genotype 0.43 0.05 location 0.00 - year 0.18 0.68 year x location 0.82 0.59 block(year x location) 0.02 0.01 location x genotype 0.03 0.03 year x genotype 0.00 - year x location x genotype 0.29 0.03 error 0.44 0.02 RIL 95 was the most desired green RIL from the population based on its average index of 4.00 across the two years and three environments. RIL 95 had a 41
Page 1 and 2: GREEN SEED COAT COLOUR RETENTION IN
Page 3 and 4: ABSTRACT Poor seed coat colour desi
Page 5 and 6: ACKNOWLEDGEMENTS The author would l
Page 7 and 8: 7.2 Maturity and Index Score ......
Page 9 and 10: Table 4.13 Acurum index score, tota
Page 11 and 12: 1.0 INTRODUCTION Green lentil has b
Page 13 and 14: example canola. In wheat it is used
Page 15 and 16: carbohydrate, and micronutrient req
Page 17 and 18: It would also be useful for lentil
Page 19 and 20: process and categorize the data int
Page 21 and 22: Table 2.2 Descriptions of the colou
Page 23 and 24: 2.4 Perceived seed colour in green
Page 25 and 26: 2.6 Cotyledon chlorophyll degradati
Page 27 and 28: temperatures. The more enzyme activ
Page 29 and 30: the seed produces bleached seeds wi
Page 31 and 32: correlation between seed weight in
Page 33 and 34: used widely as a desiccant is that
Page 35 and 36: prevailing winds. A problem that oc
Page 37 and 38: and the higher the heritability est
Page 39 and 40: 4.1 Heritability Study Objectives 4
Page 41 and 42: Plant material for the study includ
Page 43 and 44: Table 4.4 Herbicide and desiccant a
Page 45 and 46: was a=0.05. The heritability was ca
Page 47 and 48: during the time taken to remove the
Page 49: normally distributed data. In 2006
Page 53 and 54: The tester set that was included in
Page 55 and 56: etween field and phytotron index sc
Page 57 and 58: Table 4.10 shows the mean index sco
Page 59 and 60: Sutherland in 2005 produced the mos
Page 61 and 62: Table 4.12 Effect of changing the n
Page 63 and 64: wrinkling as sound seeds. It is dif
Page 65 and 66: negative relationship. The regressi
Page 67 and 68: Chlorophyll b content (mg/g) 120 10
Page 69 and 70: 1994). It is important to note howe
Page 71 and 72: estimates of which lines produced t
Page 73 and 74: in both years to produce two differ
Page 75 and 76: At the time of desiccation or swath
Page 77 and 78: a significant genotype x environmen
Page 79 and 80: Table 5.7 Mean green index score of
Page 81 and 82: All three groups for both desiccati
Page 83 and 84: index score was not equal for each
Page 85 and 86: Alternatively, the samples had less
Page 87 and 88: sclerotinia white mold (Sclerotinia
Page 89 and 90: 6.0 GENERAL DISCUSSION Knowing whic
Page 91 and 92: The Acurum ® machine has some pote
Page 93 and 94: process. If small gains in desirabi
Page 95 and 96: for more efficient selection sooner
Page 97 and 98: 8.0 REFERENCES AAFC. 2007. Agricult
Page 99 and 100: Guiamét, J.J., E. Schwartz, E. Pic
Page 101 and 102:
Shearer, S.A., G.M. Turner, M. Coll
show all

GREEN SEED COAT COLOUR RETENTION IN LENTIL - University ...

Create successful ePaper yourself

Delete template?

Save as template?