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GREEN SEED COAT COLOUR RETENTION IN LENTIL - University ...

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At the time of desiccation or swathing the maturity of the plants was noted<br />

on a 1-9 scale (Table 5.3). A score of one meant that all of the pods on the plant<br />

were green. Two represented the buckskin stage of development where the bottom<br />

one third of the plant had pods tan-coloured and were leathery to the touch. Four<br />

was considered the recommended treatment timing when the bottom third of the<br />

pods had seeds that rattled inside the pod when shaken. The middle third of the<br />

plant had pods in the buckskin stage. The top third had pods in that were still green.<br />

The numbers progress until nine where all of the pods on the plant have seeds that<br />

rattle in the pod when shaken and the plant material was completely senesced with<br />

some seeds shattering out of the pods.<br />

Table 5.3 Rating scale for maturity based on visual descriptions of lentil plants to<br />

assess maturity at pre-harvest treatment application.<br />

Scale Plant<br />

Treatment<br />

Bottom Third Middle Third Top Third timing<br />

1 green coloured pods green coloured pods green coloured pods very early<br />

2 buckskin coloured pods green coloured pods green coloured pods early<br />

3 brown coloured pods green coloured pods green coloured pods early<br />

4 seeds rattle when shaken buckskin coloured pods green coloured pods proper<br />

5 seeds rattle when shaken buckskin coloured pods buckskin coloured pods late<br />

6 seeds rattle when shaken brown coloured pods buckskin coloured pods late<br />

7 seeds rattle when shaken brown coloured pods brown coloured pods late<br />

8 seeds rattle when shaken seeds rattle when shaken seeds rattle when shaken very late<br />

9 seeds rattle when shaken seeds rattle when shaken seeds rattle when shaken very late<br />

plant material completely senesed with pods shattering<br />

A subsample of seed from each plot was analyzed for seed coat colour using<br />

the Acurum ® machine and an index value for each plot was calculated. The<br />

statistical analysis was done using SAS for Windows version 8 (SAS Institute Inc.<br />

Cary, N.C.). A mixed model was used with location, genotype, and genotype x<br />

65

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