acta societatis botanicorum poloniae - LV Zjazd Polskiego ...

one week intervals for 9 weeks. 3) Young seedlings that germinated
on salt and were still alive, were presumed to be salt tolerant.
They were transferred to 100ml jars on medium containing
175mM NaCl (or 150mM NaCl-for cvs. ‘Elkat’ and ‘Selva’) for
further continuation of selection.
2-dE-BASEd PrOTEOMIC APPrOACHES OF SOMAT-
IC EMBrYOGENESIS IN GentiAnA SPP.
Floryanowicz-Czekalska Katarzyna 1 , Rybczyński Jan J. 2
Polish Academy of Sciences, Botanical Garden – Center for
Biological Diversity Conservation, 2 Prawdziwka St., 02-973
Warsaw, Poland; 1 kasiaczeka@gmail.com; 2 jjryb@obpan.pl
Somatic embryogenesis (SE) can serve as a model system to
study the molecular events of plant embryogenesis. The process
is easily achieved in Gentiana species. The studies were done to
investigate Gentiana spp. somatic embryogenesis at the molecular
level using two-dimensional gel electrophoresis. Proteome
reference maps, initiated for somatic embryos in globular and
cotyledon-shaped stages, were compared. They revealed the
electrophoretically resolved spots in triplicate gels for each stadium
and were submitted for detection with ImageMaster 2D
Platinum (Amersham Biosciences) software. Our studies show
that 2-DE-based electrophoresis approaches can serve as tools
for identifying protein markers for the developmental stages
of Gentiana SE. The results provided a basis for future studies
including protein identification and the classification of their
functions.
HOrMONAL ANd ENVIrONMENTAL FACTOrS IN
THE PrOPAGATION OF HErBACEOUS PEONY (PAeoniA
lActiflorA PALLAS) in vitro
Gabryszewska Eleonora. Research Institute of Pomology and
Floriculture, 18 Pomologiczna St., 96-100 Skierniewice, Poland,
Department of Physiology and Morphogenesis of Ornamental
Plants, Eleonora.Gabryszewska@insad.pl
Growth regulators (cytokinins, gibberellins, auxins, JA-Me),
carbohydrates, mineral salts and temperature, influence the
growth and development of herbaceous peony in vitro. Sucrose
at a high concentration inhibited the growth and proliferation
of shoots but promoted the dormant bud and root formation.
Conversely, gibberellin in the presence of cytokinins counteracted
the sugar-induced inhibition and stimulated peony shoot
growth and multiplication. The chilling treatment reactivated
dormant shoot growth, but the effect was weaker than that of
the gibberellin treatment. Exogenous auxin at high concentrations,
inhibited shoot growth and influenced the progression of
shoots from juvenile to adult phase. The high concentration of
mineral salts strongly inhibited the growth and development of
shoots. Exogenous auxin and the high concentration of sucrose
stimulated rooting. In addition, JA-Me applied together with
IBA increased the rooting of shoots at low temperature. The
effects of growth regulators, sucrose and mineral salts in the
peony shoot proliferation and rooting were modified by temperature.
THE TECHNOLOGY OF OBTAINING CArrOT HO-
MOZYGOUS PLANTS USING ANTHEr CULTUrES
Górecka Krystyna, Krzyżanowska Dorota, Kowalska Urszula,
Kiszczak Waldemar, Górecki ryszard. Research Institute
of Vegetable Crops, 1/3 Konstytucji 3 Maja St, 96-100 Skierniewice,
Poland, krystyna.gorecka@inwarz.skierniewice.pl
At the Research Institute of Vegetable Crops in Skierniewice,
Poland the method of obtaining carrot homozygous plants by
Plant Tissue Cultures
application of anther cultures was elaborated. Embryogenic varieties
were found. An effective technique has been developed,
of plant regeneration from androgenetic embryos by inducing
secondary embryogenesis and embryo conversion. This enabled
us to eliminate the step of rooting, which was rather ineffective.
Over 90% of obtain plants had a double set of chromosomes.
Similar results of cytological and cytometric analyses,
allow us to recommend the latter, as a good tool for defining
ploidy of androgenetic carrot plants. Anatomical observations
of microspore development during the course of anther culture,
proved that embryos were formed directly from the microspores.
Homozygosity of androgenetic plants was confirmed
with isoenzymatic systems PGI and AAT. At present, the above
stages are optymalized.
IMPrOVING rOOT CULTUrES ANd in vitro CLON-
ING OF SOME HErBACEOUS ANd WOOdY PLANTS
Hanus-Fajerska Ewa, Wiszniewska Alina. University of Agriculture,
Department of Botany and Plant Physiology, 29 Listopada
54, 31-425 Cracow, Poland; e.hanus@ogr.ur.krakow.pl;
a.wiszniewska@ogr.ur.krakow.pl
The material was comprised of herbaceous plants growing in
the form of rosette (Bisculella laevigata, Brassicaceae, Armeria
maritima, Plumbaginaceae) and plants with woody shoots
(Daphne caucasica, D. jasminea, D. tangutica, Thymelaeceae).
Experiments were conducted with the aim to improve
protocols and media which permit root culture and micropropagation
of chosen models. The developed cultivation protocols
enable efficient propagation and amelioration of chosen
genotypes. It will also be possible to obtain material free from
plant pathogens.
dISTUrBANCE OF ETHYLENE BIOSYNTHESIS ANd
PErCEPTION dUrING SOMATIC EMBrYOGENESIS
IN medicAGo sAtivA L. rEdUCES EMBrYO rEGEN-
ErATION ABILITY
Kępczyńska Ewa 1 , Zielińska Sylwia. University of Szczecin,
Chair of Plant Physiology and Biotechnology; Department of
Plant Biotechnology, 13 Wąska St., 71-415 Szczecin, Poland,
1 ekepcz@wp.pl
Low germination and conversion rates, termed regeneration,
of somatic embryos to normal plantlets, are the most critical
problems limiting commercial application. In the present study,
our main objective was to determine whether controlled disturbance
of ethylene biosynthesis and perception during somatic
embryogenesis (SE) in Medicago sativa L. cv. Regalander could
influence vigor of the embryos in terms of their regeneration.
Therefore we studied the effects of the non-specific ethylene
biosynthesis inhibitors, salicylic acid (SA) and aminoethoxyvinylglicyne
(AVG) and specific inhibitors of ethylene binding
to receptors, 1-methylcyclopropene (1-MCP) and 2,5 – norbornadiene
(NBD) applied during proliferation and differentiation
phases of SE on embryogenic suspension growth, embryo production,
their development and in consequence, their germination
and conversion ability. The obtained results showed that not
only ethylene biosynthesis, but also ethylene action are involved
in the control of individual phases of SE Medicago sativa L. cv.
Regalander. Our results also showed that disturbance of those
processes during distinct phases of SE adversely affects vigor
of the somatic embryos obtained.
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