acta societatis botanicorum poloniae - LV Zjazd Polskiego ...

55th Meeting of the Polish Botanical Society, Warsaw 2010
characterized by a combination of the techniques of morphotyping
and molecular methods (sequencing of fungal ITS r DNA
region). A total of 27 mycorrhizal fungi were identified; 22 species
for L. decidua subsp. polonica and 17 species for L. decidua
subsp. decidua var. sudetica. Species richness on stands with
particular age classes was higher in Świętokrzyskie Mountains
than in Opawskie Mountains. Tomentella sublilacina and Russula
ochroleuca were the most common and dominant ECM
species for L. decidua subsp. polonica from Świętokrzyskie
Mountains while Suillus grevillei and T. sublilacina constituted
the largest part of mycorrhizas of L. decidua subsp. decidua var.
sudetica from Opawskie Mountains. There were no significant
differences in ECM species richness and composition between
forest age classes, however in both regions relative abundance
of mycorrhizas from the Russulaceae family revealed that they
had a tendency to increase with stand age. The possible reasons
for the differences between the observed ECM communities are
discussed.
TWENTY FIVE YEArS OF FUNGAL CONSErVATION
IN EUrOPE
Ławrynowicz Maria. University of Łódź, Division of Mycology,
Department of Algology and Mycology, 12/16 Banacha St.,
90-237 Łódź, Poland, miklaw@biol.uni.lodz.pl
This year the European Committee for Conservation of Fungi
celebrates its 25th Anniversary. The European Committee for
Conservation of Fungi was established during the 9th Congress
of European Mycologists in Oslo, Norway in September 1985.
The first Meeting of the Committee took place in Łódź, Poland,
in August 1988. Poland was chosen, as this country has already
had some striking achievements in the area of fungal conservation,
such as the first in Europe legal fungal species protection
(in effect since 1983) and the Red List of threatened macromycetes
published in 1986. On August 24,1989 at the 13th Congress
of European Mycologists in Tallin, the name “Committee”
was changed into “Council” and its programme was announced
as a resolution. After the establishment of the European Mycological
Association in 2003, the Council became the EMA’s
Conservation body. The main ideas, tasks and achievements of
the European Council for Conservation of Fungi, as well threats
and conservation problems of macromycetes in Poland will be
presented during the session.
MICrOSCOPIC MYCOBIOTA OF rEEd-BEd COMMU-
NITIES OF LAKE SITNO (drAWNO NATIONAL PArK,
POLANd)
Mazurkiewicz-Zapałowicz Kinga, Adamczewski Andrzej,
Ładczuk Dorota, Silicki Artur. West Pomeranian University
of Technology, Department of Hydrobiology, 4 K. Królewicza
St., 71-550 Szczecin, Poland, kinga.mazurkiewicz-zapalowicz@
zut.edu.pl
Microscopic mycobiota are the most poorly described constituents
of reed-bed species biodiversity. Therefore, in 2005– 2006
we carried out a survey on these fungal assemblages on reedbed
vegetation around lake Sitno (Drawno National Park, Poland).
From 17 host plants, nearly 500 strains of FLOs and fungi
were isolated belonging to 133 species. The mycobiota that were
most commonly observed on the plants represented the following
genera: Fusarium (10 species), Ascochyta and Stagonospora
(6 species each), Puccinia and Periconia (5 species each). The
richest biodiversity was observed on Glyceria maxima (46 taxa)
and Carex acutiformis and Phragmites australis (29 taxa each),
as well as on Schoenoplectus lacustris (25 taxa) and Acorus
calamus (24 taxa). On the remaining plants, the diversity of
mycobiota did not exceed 20 species. The species of Ascochyta
(A. antarctica, A. gracilispora, A. kirulisii, and A. melicae) as
well as Puccinia coronata and P. melicae isolated from G. max-
76
ima were found on this host plant for the first time. On the other
hand, Phyllosticta sagittifoliae and Ramularia sagittariae on
Sagittaria sagittifolia, and Phyllosticta iridum on Iris pseudoacorus,
represented novel pathogens, for the first time observed
on these plants in Poland.
CHArACTErISTICS OF FUNGI CAUSING SOOTY
BLOTCH ON APPLE IN POLANd*
Mirzwa-Mróz Ewa. Warsaw University of Life Science
– SGGW, Department of Plant Pathology, 159 Nowoursynowska
St., 02-776 Warsaw, Poland, ewa_mirzwa_mroz@sggw.pl
The aim of the study was to find out what fungi species were
responsible for sooty blotch on apples in selected regions of
Poland. During 2006– 2009 in Central, Northern and Eastern
Poland, apples with symptoms of the disease were collected.
Over one hundred isolates of fungi were obtained from the
spots of affected fruits. The identification of these isolates was
based on ITS1 rDNA sequence analysed after PCR amplification
of fungal DNA. In addition to molecular identity, the morphology
of these isolates was also examined. The results of
the morphological studies, pathogenicity of isolates and their
sequences allowed us to state that: Pseudocercosporella sp.,
Pseudocercospora sp. Peltaster sp. and Phialophora sessilis
belong to the sooty blotch fungi complex. Some selected isolates
of fungi were checked for their growth on different agar
media: PDA, CMA, MEA and Czapek. The best colony linear
growth was observed on PDA and CMA. The slowest growth
of mycelium was noted on Czapek medium.
*The State Committee for Scientific Research financially supported
this study, grant No N N310 303834.
EFFECT OF SUBSTrATE TYPE ON MACrOMYCETES
SPECIES COMPOSITION IN PINE FOrESTS IN THE
VICINITY OF OLKUSZ (SOUTHErN POLANd)
Mleczko Piotr 1 , Kapusta Paweł 2 . 1 Jagiellonian University,
Institute of Botany, 46 Lubicz St., 31-512 Cracow, Poland,
ubmleczk@cyf-kr.edu.pl; 2 Polish Academy of Sciences, W. Szafer
Institute of Botany, 46 Lubicz St., 31-512 Cracow, Poland,
p.kapusta@botany.pl
Scots pine is a dominating tree species in the forests of the
Olkusz region. It occurs mostly on sandy soil (podzol) but grows
also on dolomite substrate (post-mining waste material), where
it is planted as part of recultivation measures. The soil of recultivated
areas is characterized by high pH and high contents of
nutrients and heavy metals, therefore it creates different growth
conditions than those typical for sandy soil. The aim of the study
was to characterize the mac romycete populations in pine forests
developed on two contrasting substrates. The observa-tions
of basidiomata and ascomata occurrence were carried out during
the period 2008– 2009 on 20 permanent plots, 400 m 2 each
(14 on sandy soil and 6 on dolomite substrate). Among 156 noted
species 36 were frequent enough to be included in analysis. The
mycobiota of the two types of plots differed considerably and
were found to be strongly dependent on edaphic conditions. The
differences concerned both saprobic and ectomycorrhizal fungi.
Currently, pine ecto-mycorrhizas are being investigated. This
study was supported within the Financial Mechanism of the European
Economic Area.