acta societatis botanicorum poloniae - LV Zjazd Polskiego ...

55th Meeting of the Polish Botanical Society, Warsaw 2010
a concomitant increase in sensitivity to photoinhibition. The
ability to degrade Lhcb6 apoprotein in response to brief high
salt, wounding, high temperature and high irradiance stress,
was demonstrated here to be impaired in deg2 mutants. Therefore
our results suggest that Deg2 has an important function
in plant response to the above mentioned stresses. Apparently,
Deg2 catalyses the degradation of Lhcb6 molecules, damaged
during short time stresses.
THE INFLUENCE OF WOUNd ANd PHOSPHOrUS
dEFICIT ON SUGAr METABOLISM IN BEAN (PhAseolus
vulGAris L.)
Łukaszuk Edyta, Wąsowicz Ewelina, Ciereszko Iwona. University
of Białystok, Institute of Biology, 20b Świerkowa St.,
15-950 Białystok, Poland, edytaluk@uwb.edu.pl
Plants in the natural environment are constantly exposed to
unfavorable conditions such as pathogens, pollution, frost, mechanical
wounding or nutrient deficit. Those factors may stimulate
metabolic changes, dysfunction of cells or increase plant
resistance, in response to stress conditions. The aim of this
study was to check the influence of mechanical wounding and
phosphorus deficit on sugar metabolism and leaf photosynthetic
activity in common bean (Phaseolus vulgaris L). For the most
part, the decrease of photosynthesis in response to wound and
phosphorus deficit was observed. However, increased soluble
sugar contents in leaves but decreased soluble sugar contents
in roots was detected. Stress conditions changed the activity
of enzymes, which are involved in sucrose metabolism: acid
invertases (soluble and insoluble), sucrose synthase and UDPglucose
pyrophosphorylase. The observed changes in sugar metabolism
could be linked with acclimatization to stress conditions
caused by wounding and phosphorus deficiency.
THE IMPACT OF BIOTIC INdUCErS ON TOMATO
(lycoPersicon esculentum MILL) botrytis cinereA
INFECTION
Pietrowska Edyta, Małolepsza Urszula. University of Łódź,
Department of Plant Physiology and Biochemistry, 12/16 Banacha
St., 90-237 Łódź, Poland, ulmal@biol.uni.lodz.pl
Botrytis cinerea is a pathogen that causes gray mold disease
and the consequent loss of crop performance in tomatoes fields.
So far, it is impossible to obtain tomato varieties resistant to
this pathogen. Standard methods to combat gray mold with pesticides
do not always yield the desired results. Therefore, the
application of inducers of mobilizing natural immune defense
mechanisms makes it possible to control plant diseases. Induced
resistance is a phenomenon that helps a plant that is stimulated
to be able to trigger a metabolic response leading to its resistance
to subsequent infection. The studies compared the effectiveness
of two preparations: Prev-Am 060 SL- consisting of orange extract,
and Grevit 200 SL- consisting of grapefruit extract. The
preparations were meant to prevent and reduce the B.cinerea
impact on tomato. Plants of tomato varieties with different susceptibility
to the pathogen: Remiz – very susceptible, Corindo
– moderately susceptible, Perkoz – the least susceptible, were
treated with solutions of these preparations and then inoculated
with spores of the pathogen. The rate of appearance and size
of spots on the leaves of plants treated and untreated with the
preparations were compared. Studies have shown that spots on
the leaves of plants treated with the preparations appeared much
later and were smaller than those on untreated ones. Prev-Am
was better than Grevit at restricting the spread of the disease.
90
STIMULATING EFFECT OF CAdMIUM ON PHENYL-
PrOPANOId PATHWAY IN ArAbidoPsis thAliAnA
SEEdLINGS
Pietrowska-Borek Małgorzata1 , Nuc Katarzyna 2 . Poznań
University of Life Sciences, 35 Wołyńska St., 60-637 Poznań,
Poland; 1Department of Plant Physiology, gospi@up.poznan.
pl; 2Department of Biochemistry and Biotechnology, ktnuc@
up.poznan.pl
Experiments were carried out on the 9 day-old seedlings of
A. thaliana (Col-0) grown on a liquid medium in in vitro culture
conditions. Cd (50 μM CdCl2) was added to the medium
on the 9th day of in vitro culture. Plant material was collected
after 0,5, 1, 3, 6, 10, 18, and 24 h of treatment with Cd, and
seedlings which had not been treated with Cd were collected as
well. The activity assays and determination of the mRNA level
of 4-coumarate:CoA ligase (4CL; the key enzyme of phenylpropanoid
pathway) and chalcone synthase (CHS), were made. Additionally,
the content of anthocyanins was measured. Activity
of 4CL was measured with the use of coumarate as a substrate.
Enzyme activity increased up to 10 h from Cd application, and
was 8-fold higher than activity in the control seedlings. In the
next hours activity of 4CL decreased. The highest 4CL and
CHS gene expression was noted in the first hour of Cd acting.
From this time on, the gradual decrease of mRNA levels was
observed. Cd in the first hour caused a 5-fold and an 11-fold
increase in mRNA level for 4CL and CHS, respectively. Content
of anthocyanins reached the maximal value after 6 h and was
over 3-fold higher than in seedlings not treated with Cd. After
6 hours a decrease of anthocyanins concentration was observed.
This work was supported by grant no. N N303 068634 by the
Polish Ministry of Science and Higher Education for the years
2008– 2011.
THE rOLE OF AqUAPOrINS IN PLANT CELL rEAC-
TION TO HEAVY METALS
Przedpełska-Wąsowicz Ewa, Wierzbicka Małgorzata. University
of Warsaw, Department of Molecular Plant Physiology,
1 Miecznikowa St., 02-096 Warsaw, Poland, przedpelska@biol.
uw.edu.pl
Plant tolerance to heavy metals is known to be one of the most
interesting phenomena in biology. We know from literature
that heavy metal stress disturbs plant water relations. We also
know that on the tissue level, 75– 95% of water is transported via
aquaporins (AQPs). Therefore, we hypothesized that the level of
tolerance to heavy metals is connected to the way of regulation
of water relations at the AQPs level. We employed Cell Pressure
Probe as a main technique. Experiments were carried out on
epidermal cells of Allium cepa, Arabidopsis arenosa and Arabidopsis
halleri. We showed that Zn, Cd, Pb, Hg ions induced
AQPs closure, that this process was fast, and that this process
took place after the first several minutes. We showed also that
A. arenosa populations from metalliferous soil (highly inductive-tolerant
to heavy metals) and non-metalliferous soil (heavy
metal sensitive), differed in terms of AQPs activity. We did not
show, however, any differences between the two populations of
the highly constitutional-tolerant plant A.halleri. Zn ions affected
AQPs activity in plants from non-metallicolous population of
A. arenosa, whereas Zn and Cd ions did not affect AQPs activity
in A.halleri. Our results confirmed that AQPs play an important
role in plant tolerance to heavy metals.