acta societatis botanicorum poloniae - LV Zjazd Polskiego ...

55th Meeting of the Polish Botanical Society, Warsaw 2010
ANTIOxIdANT METABOLISM ANd PrOTEIN COM-
POSITION dUrING SALT STrESS IN MITOCHONdrIA
FrOM YELLOW LUPINE (luPinus luteus L.)
EMBrYO AxES
Wojtyla Łukasz 1 , Kosmala Arkadiusz 2 , Róg Michał 1 , Garnczarska
Małgorzata 1 . 1 Adam Mickiewicz University, Faculty
of Biology, Department of Plant Physiology, 89 Umultowska St.,
61-614 Poznań, Poland; wojtylal@amu.edu.pl; michal.welniak@
gmail.com; garnczar@amu.edu.pl; 2 Polish Academy of Sciences,
Institute of Plant Genetics, 34 Strzeszyńska St., 60-479
Poznań, Poland, akos@igr.poznan.pl
Salt stress is one of the major abiotic stresses. High concentration
of salt results in an increase in the amount of ROS which
could lead to PCD. Plant cells involve physiological and molecular
processes to cope with stress conditions. All adaptive
processes require an energy supply. Mitochondria are the main
site of energy production in cells. Thus, in our study we have
investigated changes in mitochondria from embryo axis during
salt stress conditions. Isolated lupine embryo axis were grown
on modified Heller medium with or without the addition of 0,1M
NaCl, for 24h and 48h. Changes in abundance of mitochondrial
proteins in lupine embryo axis were investigated using the 2D-
IEF-SDS-PAGE technique. The protein spots with the highest
differences were chosen to be sent for identification. Native
electrophoresis and activity analysis of catalase and ascorbic
peroxydase revealed changes in their activity. DNA laddering
and cytochrome c leakage were also observed during salt stress
as symptoms of PCD. This work was partially supported by the
Polish Ministry of Science and Higher Education research grant
N N303 471038.
ACId PHOSPHATASES ACTIVITY ANd GrOWTH OF
THrEE BArLEY (hordeum vulGAre L.) CULTI-
VArS UNdEr dIVErSE PHOSPHOrUS NUTrITION
żebrowska Ewa 1 , ruminowicz Marta, Ciereszko Iwona 2 .
University of Bialystok, Institute of Biology, Departament of
Plant Physiology, 20b Swierkowa St., 15-950 Bialystok, Poland;
1 ewaw@uwb.edu.pl; 2 icier@uwb.edu.pl
Three barley cultivars (Promyk, Skald and Status) were grown
for 1– 3 weeks on different nutrient media with contrasting phosphorus
source: inorganic – KH 2PO 4 (control), organic – phytic
acid and with no phosphate (-P). Growth parameters, acid phosphatase
activity, inorganic phosphate (P i) and total phosphorus
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(P c) content were measured. Additionally, protein extracts from
tissues (shoots and roots) were run on native discontinuous gel
electrophoresis to determine acid phosphatases isoforms. Acid
phosphatase activity localization was determined in root cross
sections. Phosphate deficiency in nutrient medium decreased
shoot and root elongation, whereas barley cultivars grown on
phytic acid showed a similar growth to that of the control plants.
Phosphate starvation led to a decrease of P i (and P c) in shoots
and roots of all the studied barley cultivars already during the
first week of culture, but in plants grown on phytic acid P i and
P c content was similar to that of the control plants. Extracellular
acid phosphatase activity in plants grown on –P medium
was not enhanced, and in plants grown on phytic acid the activity
was even lower than in the control plants. This work was
supported by a grant from the Polish Ministry of Science and
Higher Education (2007– 2010).
INFLUENCE OF dIVErSE PHOSPHOrUS NUTrITION
ANd MYCOrrHIZATION ON GrOWTH OF OAT (AvenA
sAtivA L.)
żebrowska Ewa 1 , Milewska Marta, Ciereszko Iwona 2 . University
of Białystok, Institute of Biology, Departament of Plant
Physiology, 20b Świerkowa St., 15-950 Białystok, Poland;
1 ewaw@uwb.edu.pl; 2 icier@uwb.edu.pl
Oat cultivars (Arab and Krezus) were grown for 4 weeks on
sand with: soluble, inorganic source of phosphorus – KH 2PO 4
(control, +P), insoluble, inorganic phosphate – FePO 4, organic
– phytic acid and without phosphorus (-P). Effects of diverse
phosphorus source and mycorrhizal fungi (Glomus intraradices)
inoculation on growth parameters, inorganic phosphate
(P i) and total phosphorus concentration (P c) were studied.
In all the treatments, we observed a decreased P i content in
shoots and roots of both oat cultivars in comparison to the control
plants (except shoots of +P plants inoculated with mycorrhizal
fungi cv. Krezus where P i content was increased). The
shoot fresh mass of both cultivars, cultured without phosphate
or with FePO 4 was decreased (except –P plants inoculated
with mycorrhizal fungi). Whereas plants grown with phytic
acid had similar, or even higher (Krezus cv.) shoot and root
fresh mass as did the +P plants. Effect of mycorrhization
on growth of both oat varieties was insignificant with most
phosphorus nutrition treatments. This work was supported by
a grant from the Polish Ministry of Science and Higher Education
(2007– 2010).