acta societatis botanicorum poloniae - LV Zjazd Polskiego ...

55th Meeting of the Polish Botanical Society, Warsaw 2010
ALPHA-GALACTOSIdASE ACTIVITY IN GErMINAT-
ING ZYGOTIC ANd SOMATIC EMBrYOS OF medicAGo
sAtivA L. ANd ITS rEGULATION BY ENdOG-
ENOUS GIBBErELLINS ANd ETHYLENE
Zielińska Sylwia 1 , Kępczyńska Ewa. University of Szczecin,
Chair of Plant Biotechnology, Department of Plant Physiology
and Biotechnology, 13 Wąska St., 71 415 Szczecin, Poland,
1 sziel@univ.szczecin.pl
It is known that seeds and somatic embryos in good vigor should
contain an adequate quantity of storage materials. Efficiency of
their germination depends largely on the hydrolysis speed of
these materials connected with the activity of basic enzymes:
α-amylase, α-galactosidase. The activity of α-galactosidase, the
enzyme that is responsible for soluble sugars hydrolysis, in unimbibed
and germinating zygotic and somatic embryos of Medicago
sativa L. cv. Rangelander, was detected. The dynamism
of enzyme activity was analyzed in the I phase of germination
(imbibition) after 16, 18, 20, 24 and 48 h. Dry somatic embryos
as compared to zygotic had 2.5-fold higher α-galactosidase activity.
However during imbibition the activity of this enzyme
increased faster and was widely higher in germinating zygotic
embryos of alfalfa than in somatic. We tested whether known
stimulators of seed germination (gibberellins and ethylene) may
regulate the activity of this enzyme during the first phase of germination.
For this purpose inhibitor of gibberellins biosynthesis
– ancymidol (ANC) and inhibitors of ethylene biosynthesis
– aminoethoxyvinylglycine (AVG) and salicylic acid (SA) were
applied. The inhibitory effects of ancymidol, AVG and SA indicated
that endogenous gibberellins and ethylene are required for
regulation of α-galactosidase activity.
EMPLOYMENT OF in vitro CULTUrES IN chAmAedAPhne
cAlyculAtA (L) MOENCH PrOTECTION
źróbek-Sokolnik Anna, Kucewicz Magdalena, Hołdyński
Czesław. University of Warmia and Mazury in Olsztyn, Department
of Botany and Nature Protection, 1 Łódzki Sq., 10-727
Olsztyn, Poland, a.zrobeksokolnik@uwm.edu.pl
Leather leaf Chamaedaphne calyculata Moench (L) in Poland
is rare and under full legal protection. It is a wintergreen dwarf
shrub in the Ericaceae family. The limited number of existing
stands of this plant (nowadays 9 small and widespread stands
from 13 historically known stands) is the reason it is important
to determine the possibility of propagating Chamaedaphne calyculata
for the purpose of ex situ protection. The above reasons
were the signal for starting experiments, conducting elaboration
of protocol for in vitro propagation of leather leaf. The materials
used were plant material derived from plants growing in the
Mazurski Landscape Park (showing one of the lowest genetic
erosion levels from among the Polish populations). These were
the goals: 1) optimal time for plant material harvesting; 2) selection
of optimal sterilization method; 3) the most useful explant
type and medium for micropropagation via shoot organogenesis;
and 4) elaboration of the method used for plantlet acclimatization
to ex vitro conditions. On September 2009, 6 plantlets
derived from in vitro cultures, after acclimatization in growth
chamber and glasshouse, were transferred to Leśne Arboretum
in Kudypy near Olsztyn.