the Expedition ARKTIS-XIX/4 of the research vessel POLARSTERN ...

More documents

Recommendations

Info

42 Station Latitude Longitude Date Location PS64 HELI 01-1 76°07.61’N 18°36.10’W 13.08.03 Store Koldewey – Melles Lake PS64 HELI 01-2 76°06.82'N 18°30.51'W 13.08.2003 Store Koldewey – Shore PS64 HELI 02-1 76°22.95'N 04°38.32'W 15.08.2003 Ice floe PS64 HELI 02-2 76°22.80'N 04°39.26'W 15.08.2003 Ice floe PS64 HELI 03-1 76°55.07'N 06°31.73'W 16.08.2003 Ice floe PS64 HELI 04-1 76°44.99'N 05°28.80'W 16.08.2003 Ice floe PS64 HELI 04-2 76°44.80'N 05°27.52'W 16.08.2003 Ice floe PS64 HELI 05-1 77°09.01'N 01°10.30'W 20.08.2003 Ice floe PS64 HELI 06-1 77°08.98'N 01°12.06'W 20.08.2003 Ice floe PS64 HELI 07-1 75°35.14'N 08°02.86'W 25.08.2003 Ice floe PS64 HELI 08-1 75°03.48'N 18°45.86'W 26.08.2003 Shannon Island – Potsdam Sø PS64 HELI 08-2 75°05.97'N 17°51.86'W 26.08.2003 Shannon Island – Frosnebugt, Shore PS64 HELI 09-1 74°58.54'N 19°58.71'W 29.08.2003 Kuhn Ø – Shore PS64 HELI 09-2 75°00.75'N 20°06.08'W 29.08.2003 Kuhn Ø – Shore PS64 HELI 09-3 75°10.26'N 19°58.96'W 29.08.2003 Hochstetter Forland – Shore PS64 HELI 09-4 75°11.49'N 19°59.85'W 29.08.2003 Hochstetter Forland – Karls Pynt, Shore PS64 HELI 10-1 75°38.67’N 09°12.81’W 04.09.2003 Ice floe PS64 HELI 10-2 75°41.85’N 08°55.79’W 04.09.2003 Ice floe PS64 HELI 11-1 76°06.88’N 08°44.67’W 05.09.2003 Ice floe PS64 HELI 11-2 76°13.00’N 08°14.72’W 05.09.2003 Ice floe PS64 HELI 11-3 76°12.97’N 08°59.94’W 05.09.2003 Iceberg PS64 HELI 12-1 75°42.23’N 19°36.13’W 06.09.2003 Hochstetter Forland – Roseneathbugt, Shore PS64 HELI 12-2 75°37.31’N 19°44.12’W 06.09.2003 Hochstetter Forland – Lake PS64 HELI 12-3 75°15.66’N 20°54.28’W 06.09.2003 C.H. Ostenfelds Land – Kildedalen, Shore PS64 HELI 13-1 74°08.29’N 21°25.80’W 08.09.2003 Clavering Ø – Granatdal, Shore PS64 HELI 13-2 74°10.19’N 22°19.57’W 08.09.2003 Hansen Havn – Wordies Gletscher PS64 HELI 13-3 73°57.02’N 21°10.51’W 08.09.2003 Hold With Hope – Stensiö Bjerg, Shore PS64 HELI 14-1 73°23.99’N 24°15.39’W 14.09.2003 Ymers Ø – Zoologdalen, Shore PS64 HELI 14-2 73°21.42’N 23°49.05’W 14.09.2003 Ymers Ø – Gunnar Anderssons Land, Shore Tab.4.2-1: List of geological stations on ice floes / icebergs and on land during the expedition ARK- XIX/4a. 4.3 Molecular Biology of Benthic Foraminifera ARK XIX/4a Martina Blümel Sampling Samples for genetic analysis were taken by a Multicorer equipped with 8 plastic tubes of 10 cm inner diameter. An overview over the samples taken for genetic analysis is given in tab.4.3-1. Before sampling the tubes sediment surface temperature was measured to get information about the in situ living conditions of the benthic foraminiferal community. After temperature measurements, the water supernatant was taken away by a hose and preserved.
A surface sediment sample was taken from 2-3 Multicorer tubes. The uppermost centimeter of each tube was taken and immediately sieved under cold seawater after sampling. 5 fractions were gained: >2mm, >500µm, >200µm, >112µm and >63µm. Samples were put in 100 ml Kautex bottles, filled with the supernatant water of the Multicorer tube and immediately frozen at –20°C until further processing. Methodology For further treatment, the sample was thawed and a sub sample was examined using a stereomicroscope with a Nikon Coolpix 995 digital camera. Samples were sorted in a Petri dish containing water using a brush. Specimens considered as „alive hat the moment of sampling“ were picked out and subjected to further treatments. Characters for the physiological state of the organisms are: intact state of the test/ mechanical damage, first chamber empty, other chambers filled, extended pseudopodia (not observed), attachment to stones or other organisms, colour and state of the first chamber (agglutinated specimens), also see figures 4.3-1 and -2 for details. Specimens were determined using classical morphological methods and Robert Wynn Jones´ „The Challenger Foraminifera”. Additionally specimens were documented by the Nikon Coolpix 995 camera before further treatments. DNA-Extraction After sorting and documentation each single specimen was put into an 1.5 ml Eppendorf-cup and 40 µl DOC-solution were added. For DNA-extraction, the cells were crushed by mini-pistils and the samples were incubated for 1h at 60°C. After incubation, samples were frozen at –20°C until further processing. As a whole, 376 specimens were extracted and treated by PCR. PCR PCR (Polymerase Chain Reaction) is a cyclic process to amplify a selected DNAfragment between two primers (Primer: short DNA fragments used as start and end point in conservative DNA regions). For phylogenetic analysis of relationships among eukaryotes, the gene encoding the small subunit of the ribosomes (18S rDNA, SSU rDNA) is commonly used. Primers used here were specifically designed for analysis of the foraminiferal 18S rDNA. PCR was conducted using two different primer pairs, Cibi fwd/Cibi rev for rotaliid foraminifera and F4/R1b for Astrorhizidae and Textulariidae. PCR was performed using puRE Taq Ready-to-Go PCR Beads from Amersham Biosciences and a Techne Progene thermocycler with the following protocol: Initial Denaturing: 94°C for 2 min Primer Annealing: 55°C for 40 sec Elongation: 72°C for 1 min 20 sec Denaturing: 91°C for 40 sec Final Elongation: 72°C for 5 min Cooling: 4°C 43
Page 1: the Expedition ARKTIS-XIX/4 of the
Page 5 and 6: 1 Cruise summary W. Jokat The exped
Page 8 and 9: 6 1.1 Wetterverlauf ARK XIX/4a Bei
Page 10 and 11: 8 noch vor der Küste, fern davon a
Page 12 and 13: 10 northerly wind increased steadil
Page 14 and 15: 12 In the latest stages of the proj
Page 16 and 17: Profile Date/Time Start Date/Time T
Page 18 and 19: Station Typ Extra Lat Long Höhe [m
Page 20 and 21: Station Typ Extra Lat Long Höhe [m
Page 22 and 23: Date Flight Start Time End Time No
Page 32 and 33: 30 Preliminary results Along profil
Page 34 and 35: 32 In addition to the deep seismic
Page 36 and 37: 34 Fig. 2-5: Flight statistics for
Page 38 and 39: 36 Fig. 3-1: Seafloor topography of
Page 40 and 41: 38 In total, the PARASOUND system o
Page 42 and 43: 40 Figure 4.1-3: Example of PARASOU
Page 46 and 47: 44 After PCR was finished, samples
Page 48 and 49: 46 Potsdam Lake (Shannon Island) Sc
Page 50 and 51: 48 In den Schmelzwassertümpeln kon
Page 52 and 53: Temp. Sal. Tiefe max. Tiefe Trübe
Page 54 and 55: 52 Store Koldewey Store Koldewey is
Page 56 and 57: 54 In the shallow ponds the hydrolo
Page 58 and 59: 56 The low amounts of solubles in t
Page 60 and 61: 58 lake core no. latitude longitude
Page 62 and 63: 60 6.3 Diatom phytoplankton and phy
Page 64 and 65: 62 Generally, all sampled benthic s
Page 66 and 67: 64 shaped profile at the top were i
Page 68 and 69: 66 depth horizon description sample
Page 70 and 71: 68 measured in the field, and the c
Page 72 and 73: 70 Summary of results The summit pl
Page 74 and 75: 72 Pre-Holocene shell material Pre-
Page 76 and 77: 74 Christensen, T.R. (1993): Methan
Page 78 and 79: 76 No EVK2-2001-00215 (ASOF-N). The
Page 80 and 81: 78 Altimeters Model 2110-2, SN 189
Page 82 and 83: 80 Mooring Latitude Longitude Water
Page 84 and 85: 82 Mooring Latitude Longitude Water
Page 86 and 87: 84 30° W 20° W 30° W Greenland 2
Page 88 and 89: Fig. 7-4: Vertical transects of pot
Page 90 and 91: 88 8 Bericht zur akustischen Vermes
Page 92 and 93: 90 Quality control sheet - Profile
Page 94 and 95:
92 Quality control sheet - Profile
Page 96 and 97:
Station List Station Date Time Posi
Page 98 and 99:
Station Date Time PositionLat Posit
Page 100 and 101:
Page 102 and 103:
Page 104 and 105:
Page 106 and 107:
Page 108 and 109:
Page 110 and 111:
Page 112 and 113:
Page 114 and 115:
Page 116 and 117:
Page 118 and 119:
Page 120 and 121:
Page 122 and 123:
Page 124 and 125:
Page 126 and 127:
Page 128 and 129:
Page 130 and 131:
Page 132 and 133:
Page 134 and 135:
Page 136 and 137:
Page 138 and 139:
Page 140 and 141:
Page 142 and 143:
Page 144 and 145:
142 Beteiligte Institutionen / Part
Page 146 and 147:
144 Fahrtteilnehmer / Participants
show all

the Expedition ARKTIS-XIX/4 of the research vessel POLARSTERN ...

You also want an ePaper? Increase the reach of your titles

Delete template?

Save as template?