liiiMIIIfl~UDliiiMIII~U - Biblioteca de la Universidad Complutense ...

Vol. 224, No. 3, 1996 BIOCHEMICAL MW BYOPHYSICAL RESEARCH COMMUNICATIONS
o
140
I-;4 120
1—
o
o
1 00
140
~ 120
0
‘-4
oo
100
-1 0
Iog [taxol] (gM)
-2 -1 0
log [OA]«¡M)
HG. 1. Taxol anuagamzes te OA-¡aduced sdmulañon of CPT-L Panel A: Hepatacytes were preincubated far30
mm with vazyung concentraríons of mxci, follawed by 15 additional mía ¡a ¡he absence (O) & ¡a ¡he presence of 0.5
~¿l.4OA (e). Panel E: Heparocyws were preuncubared for 30 miii ¡a te absence (O) or ¡a te presence af 10 sM
mal (e), follawed by 15 addiflanai mía with varyung concentañons of OX la bat paneis, CFI-Y acúvity vas
derermuned ¡a digitanin-permeabilized heparocytes foliawing ¡he uncubañans of ¡he celis ¡a ¡he presonce or absence
of te ¡adicared agonista. Ya bat cases values correspand to 3 separare bepatacyte preparatians.
cytes wit QA resulted un a decreased retendon oflactate dehydrogenase lix dxc permeabilized
cells. Once again, taxol prevented this effea of QA (Table 2).
Altough QA is a mare potent inhibitar of pratein plxasphatase 2A tan of protein
phosphatase 1, u te doses emplayed in dxc present study (0-5 gM) QA is supposcd ta
completely inbibit both proteun phosphatases (22,23). Ta study wbeter te stimulatory
756