liiiMIIIfl~UDliiiMIII~U - Biblioteca de la Universidad Complutense ...
liiiMIIIfl~UDliiiMIII~U - Biblioteca de la Universidad Complutense ...
liiiMIIIfl~UDliiiMIII~U - Biblioteca de la Universidad Complutense ...
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LOSS OF RESPONSE OF CARNITINE PALMITOYLTRANSFERASE 1<br />
TO OKADAIC ACID IN TRANSFORMED HEPATIC CELLS<br />
Guillermo Ve<strong>la</strong>sco’, Patricia Passilly’, Manuel Guzmán~ and Norbert Latruffe’<br />
‘Department af Biachemistry arad Molecu<strong>la</strong>r Bio<strong>la</strong>gy 1, Faculry of Biology, Compluterase University, 28040<br />
Madrid, Spain; arad ‘Laboratoire <strong>de</strong> Bia<strong>la</strong>gie Molecu<strong>la</strong>ire et Cellu<strong>la</strong>ire, Faculté <strong>de</strong>s Sciences Miran<strong>de</strong>,<br />
Université <strong>de</strong> Bourgagne, BP 400, 21011 Dijon, France<br />
ABSTRACr. The specific activity of carnitine palmitoyltransferase 1 (CPT-I) was simi<strong>la</strong>r in mitacharadria<br />
isa<strong>la</strong>ted fram rat Fao and human HepG2 heparama celís arad from rat hepatocytes, bur almost 2-foid<br />
higber in permeabilized hepatoma celís thara ira permeabilized heparocytes. Short-rerm exposure ta akadaic<br />
acid induced a ca. 80% stimu<strong>la</strong>tion of CPT-I ira hepatocytes, whereas no significant response of the enzyme<br />
from hepatoma celís was evi<strong>de</strong>rat. Thus, libe high CPT-I activir>’ disp<strong>la</strong>yed by hepatama celis may be<br />
reached by hepatocyliesupan challerage ta okadaic acid. The present data may be exp<strong>la</strong>ined by a disruption<br />
of irateractioras beliweera CPT-I arad cytoskeletal componerats ira tumor celís wbicb may be involved ira lihe<br />
okadaic acid-induced activatian of hepatie CPT-I as previausly suggested [BiochemBiophys Res Commun<br />
224~ 754-759, 1996].<br />
Mitochandrial fatty acid axidatian provi<strong>de</strong>s a major saurce of ener~’ ira beart, skeletal muscle arad liver<br />
(reviewed ira [1-3]).Hepatic fatty acid oxidarion alsa supplies exírahepatie tissues with kerone bodies as<br />
a glucase-rep<strong>la</strong>cing fuel[1-3].Carnitine palmitayltrarasferase 1 (CPT-I), the auter-mitachoradrial-membrane<br />
carnitine palmitoyltransferase, calialyzes libe paco-settirag step of long-chaira fatty acid transiacarian inta<br />
Abbrev<strong>la</strong>tlons: CPT-I, carnitine palmitayitransferase 1; ODH, glutamate <strong>de</strong>hydrogenase<br />
torresponding autitor: Dr. Manuel Guzmán, Deparlimerat of Biocheniistry arad Molecu<strong>la</strong>r Biology 1, FaculÉ>’ of Biology,<br />
<strong>Complutense</strong> Uraiversit>’, 28040-Madrid, Spain. Telephone: (1) 3944671. Far (1) 3944672. E-mail: mgp@solea.quim.ucm.es
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