CamSep 3 S

APPLICATION OF MICRO-TLC PLATFORM FOR FRACTIONATION
OF HIGHLY ORGANIC COMPOUNDS LOADED MATERIALS
Paweł K. ZARZYCKI
/Stosowana w pracy technika rozdzielania: micro-TLC, HPLC/
Section of Toxicology and Bioanalytics, Department of Civil and Environmental Engineering,
Koszalin University of Technology, Śniadeckich 2,75-453 Koszalin, Poland
The main scope of this communication is to summarize the latest research of our group
concerning application of micro-thin-layer chromatography (micro-TLC) as a simple fractionation
tool for fast screening of raw extracts derived from complex biological, pharmaceutical and
environmental samples [1-5]. The problem of qualitative and quantitative determination of bioactive
substances from highly organic compounds loaded materials will be discussed from practical point
of view. Particularly, results of fractionation of complex matrices originated from food samples,
birds’ feathers, fatty oils, milk components, fresh and hydrolyzed fish bile as well as soot residues
in dust samples derived from biomass fuel and fossils home heating systems will be presented.
Moreover, chemometric investigations based on micro-TLC involving fluorescence detection and/or
PMA visualization of SPE extracts derived from surface water ecosystems and treated sewage water
samples discharged by the municipal wastewater treatment plant will be reported in comparison
with UV-DAD HPLC generated data [6, 7].
References:
[1] M.J. Baran, P.K. Zarzycki, “Fast method for fractionation of lipids and related non-polar substances
from birds’ feathers using thermostated micro-TLC”, Camera Separatoria, 3 (1) (2011) 221-227.
[2] P.K. Zarzycki, M.M. Ślączka, M.B. Zarzycka, E. Włodarczyk, M.J. Baran, T. Heese, B.K. Głód, “Fast
separation and detection of main components in complex raw biological materials using temperaturecontrolled
planar micro-chromatography (micro-TLC)”, Measurement Automation and Monitoring
(Pomiary Automatyka Kontrola), 56 (4) (2010) 360-364.
[3] P.K. Zarzycki, M.M Ślączka, M.B. Zarzycka, M.A. Bartoszuk E. Włodarczyk, M.J. Baran;
“Temperature-controlled micro-TLC: a versatile green chemistry and fast analytical tool for separation
and preliminary screening of steroids fraction from biological and environmental samples”, Journal of
Steroids Biochemistry and Molecular Biology, (2011) DOI: 10.1016/j.jsbmb.2011.05.007.
[4] P.K. Zarzycki, M.B. Zarzycka, V.L. Clifton, J. Adamski, B.K. Głód, “Low parachor solvents extraction
and thermostated micro-TLC separation for fast screening and classification of spirulina from
pharmaceutical formulations and food samples”, J. Chromatogr. A, 1218 (2011) 5694-5704.
[5] P.K. Zarzycki, M.M. Ślączka, M.B. Zarzycka, E. Włodarczyk, M.J. Baran; “Application of micro-thinlayer
chromatography as a simple fractionation tool for fast screening of raw extracts derived from
complex biological, pharmaceutical and environmental samples”, Anal. Chim. Acta, 688 (2011)
168-174.
[6] P.K. Zarzycki, E. Włodarczyk, M.J. Baran, “Determination of endocrine disrupting compounds using
temperature-dependent inclusion chromatography I. Optimization of separation protocol”,
J. Chromatogr. A, 1216 (2009) 7602-7611.
[7] P.K. Zarzycki, E. Włodarczyk, M.J. Baran; “Determination of endocrine disrupting compounds using
temperature-dependent inclusion chromatography II. Fast screening of free steroids and related lowmolecular-mass
compounds fraction in the environmental samples derived from surface waters, treated
and untreated sewage waters as well as activated sludge material”; J. Chromatogr. A, 1216 (2009)
7612-7622.
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