Transcriptional Characterization of Glioma Neural Stem Cells Diva ...

6.4 Core Differentially Expressed Genes Results
Figure 6.11: NTRK2 is a brain-derived neurotrophic factor detected in the literature
in a subset of cells in astrocytomas [29] and found in our Tag-seq dataset
by mappings of tags on the shortest isoform. Three tracks are visible in the figure:
at the top, a customised track composed of 12 sub-tracks representing the expression
levels of our GNS cell lines and NS cell lines in red - for tag mappings on the
minus strand - and blue - for tag mappings on the plus strand. The level of tag
expression is indicated by the thickness of the line representing the mapped tag; at
the centre a track representing UCSC genes, RefSeq genes and Ensembl genes; at
the bottom a track showing the zoom in of the central track region where the tag
mapping takes place. The thicker lines in the bottom track indicate the 3’UTRs
of the gene. The tags representing the NTRK2 gene map onto a region that identifies
both the shorter - potentially the one that lacks the kinase domain - and a
longer isoform. Adapted from the UCSC genome browser (our Tag-seq track for
hg19 is added by pasting the following line into the custom track box http://gns:
zed9epre@www.ebi.ac.uk/\~engstrom/gns/tracks/hg19/gns\_tpm.bg.gz).
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