Transcriptional Characterization of Glioma Neural Stem Cells Diva ...

6.5 Large-scale qRT-PCR Validation Results
Figure 6.12: Expression estimates correlate well between Tag-seq and qRT-PCR.
(a) Expression values for MMP17, DDIT3, MYL9 and MAN1C1 in two GNS and
two NS lines assayed by both technologies. Note the near-perfect agreement between
Tag-seq (x-axis) and qRT-PCR (y-axis) over a wide dynamic range. (b) Histogram of
correlation between Tag-seq and qRT-PCR for each of the 82 genes measured by qRT-
PCR. (c) Fold-change estimates (indicating expression level in GNS lines relative to
NS lines) from Tag-seq and qRT-PCR for the 82 genes. qRT-PCR confirmed greater
than two-fold difference in expression (dashed lines at y = 1) for all genes.
Figure 6.13: Heatmap of 29 genes differentially expressed between 16 GNS and 6
NS cell lines. Colours indicate δδCt expression values, i.e. normalised expression
on a log 2 scale where zero corresponds to the average expression between the two
groups (GNS and NS).
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