Transcriptional Characterization of Glioma Neural Stem Cells Diva ...

A.3 Quantitative RT-PCR Appendix
Table A.4: Normalised Ct values. Abbreviations: "down" for down-regulated, "up" for up-regulated, and "Norm" for Normalisation control.
Well Applied Biosystems assay ID Gene CB130 CB130 CB130 CB152 CB152 CB152 CB152 CB171 CB171 CB171 CB192 CB541 CB660 CB660 G2 G2 G7 G7 G7 G7 G9 G9 G14 G14
Category A B C A B C D A B C A B A B A B C D A B A B
1 MYL9-Hs00382913_m1 Core down 28.2 27.9 28.2 23.0 25.2 25.6 25.3 29.4 30.2 29.5 26.8 24.9 27.2 26.7 36.1 33.4 31.9 36.0 30.5 29.4 26.3 26.6 35.0 35.2
2 CHCHD10-Hs01369775_g1 Core up 27.1 25.7 26.1 25.9 25.3 24.6 24.5 27.2 26.5 26.1 28.8 29.7 29.3 29.5 24.7 23.1 26.2 25.7 25.6 24.7 25.5 26.3 27.1 26.9
3 RGS5-Hs00186212_m1 Core down 31.3 30.4 30.4 29.2 31.7 30.4 29.3 32.9 29.5 30.8 32.6 27.4 26.5 27.6 30.4 29.4 31.5 30.9 28.4 27.6 30.5 31.8 32.4 32.1
4 ST6GALNAC5-
Core down 28.9 28.8 28.6 33.5 33.7 32.4 31.9 29.8 30.5 29.5 25.6 26.7 23.4 24.9 35.2 33.3 36.4 36.8 34.9 34.7 28.6 28.9 31.1 29.9
Hs00229612_m1
5 CEBPB-Hs00270923_s1 Core up 33.1 30.7 30.6 30.3 31.8 31.2 31.3 32.9 30.3 31.3 30.7 32.0 32.6 31.3 31.6 32.7 29.1 29.8 29.8 29.6 29.6 30.2 30.7 30.1
6 C5orf13-Hs00854282_g1 Core down 28.5 27.7 28.1 25.5 27.3 26.4 25.6 28.5 25.2 26.6 29.1 24.8 25.2 26.2 36.1 25.2 30.3 29.0 22.8 26.9 27.3 36.2 27.8 31.5
7 PDGFRA-Hs00998026_m1 Marker 37.4 37.4 34.8 33.2 36.8 27.9 28.4 37.5 35.4 36.9 32.4 35.7 29.9 32.1 24.2 24.3 23.2 22.2 21.5 22.5 24.0 24.1 25.9 26.2
8 CCND2-Hs00922419_g1 Core down 32.3 30.8 31.0 31.6 33.7 28.4 28.5 32.4 31.1 30.9 25.1 24.1 24.1 24.7 22.5 21.3 22.7 22.0 23.9 23.2 30.4 29.9 34.1 37.3
9 NKX2-1-Hs00163037_m1 Core down 37.4 37.4 37.1 37.1 36.8 36.4 37.2 37.5 35.4 36.9 37.0 28.8 29.0 29.8 36.1 34.4 32.8 34.6 32.2 32.4 36.7 36.2 31.2 31.3
10 CTSC-Hs00175188_m1 Core up 23.5 24.6 24.6 24.6 24.0 24.7 24.3 23.5 24.3 24.0 23.9 25.1 25.5 23.7 20.6 20.0 25.3 24.4 26.5 25.1 23.0 23.5 23.4 23.2
11 18S-Hs99999901_s1 Norm 13.7 12.0 11.5 12.2 13.8 10.9 11.3 13.4 10.8 11.2 12.5 12.8 12.1 12.1 14.0 16.4 12.5 12.8 10.4 11.6 12.6 14.0 12.7 12.6
12 DNER-Hs00294564_m1 Core down 32.3 32.3 32.8 32.7 31.5 28.1 27.5 32.2 29.9 32.0 30.4 22.3 22.4 22.7 25.5 23.3 27.8 25.2 23.5 25.0 26.5 24.0 32.5 31.5
13 LMO4-Hs01086790_m1 Core up 27.0 26.8 26.9 26.7 27.5 26.6 25.4 26.9 26.3 27.1 25.7 26.1 25.5 26.7 22.5 21.3 22.2 22.4 24.0 23.7 24.4 25.7 25.1 25.0
14 PLCH1-Hs00392783_m1 Core down 26.5 26.3 26.2 26.4 26.8 27.3 26.3 26.2 26.4 27.5 26.8 27.0 27.8 28.7 32.6 32.2 29.5 29.9 29.1 28.9 27.9 29.1 27.2 26.8
15 SPARCL1-Hs00949886_m1 Core down 33.6 31.2 31.0 27.0 29.5 24.1 22.2 35.6 28.3 30.3 28.5 25.3 22.8 25.6 24.5 23.1 24.4 27.6 29.1 29.7 22.4 25.4 28.9 28.1
16 SYNM-Hs00322391_m1 Core down 29.3 28.2 28.1 26.8 27.3 27.3 25.7 28.6 27.3 28.6 27.1 27.6 27.1 27.2 28.6 27.7 29.0 29.5 28.7 28.6 25.2 27.8 33.7 34.5
17 FBLN2-Hs00157482_m1 Core down 37.4 36.2 37.1 36.0 36.8 36.4 35.1 37.5 33.6 35.5 33.8 29.1 27.5 29.2 33.5 33.4 35.4 32.7 36.2 35.3 33.2 36.2 36.8 36.2
18 SALL2-Hs00413788_m1 Core down 37.4 33.4 33.5 31.0 36.7 30.1 27.2 37.4 28.7 33.1 32.8 26.5 24.5 27.9 30.5 31.2 28.1 28.5 28.9 30.6 34.6 36.2 31.6 30.3
19 TUSC3-Hs00185147_m1 Core down 24.4 22.8 23.0 23.3 23.6 22.7 21.8 24.4 23.5 23.3 24.4 23.4 23.4 23.9 36.1 34.4 35.7 33.7 36.3 34.2 23.3 22.9 24.0 23.8
20 GPR158-Hs00393109_m1 Core down 34.8 34.5 34.9 37.0 36.3 31.4 32.6 36.0 34.5 33.3 26.9 25.6 27.1 26.6 26.3 26.4 28.2 26.6 27.9 26.6 34.6 34.8 32.6 32.3
21 PEG3-Hs00377844_m1 Core down 31.0 31.4 31.2 30.0 30.7 30.3 29.4 30.4 29.6 30.9 29.8 28.1 25.2 27.9 27.2 27.0 26.5 24.6 25.6 26.3 33.3 35.8 29.0 29.0
22 FUT8-Hs00189535_m1 Core up 26.6 26.7 26.8 26.3 26.7 27.4 26.4 27.0 27.1 27.1 26.4 27.9 28.4 27.1 25.2 24.0 27.9 27.3 26.5 27.3 25.6 26.5 28.5 28.3
23 HLA-DRA-Hs00219575_m1 Core up 27.8 26.5 26.4 28.3 29.8 25.3 22.3 29.1 25.4 27.4 22.3 27.2 30.8 26.4 20.5 20.6 24.7 24.0 23.9 23.6 21.1 25.2 27.6 28.0
24 ASCL1-Hs00269932_m1 Marker 34.3 32.4 31.9 32.8 35.7 27.5 26.3 35.0 30.5 35.0 32.7 29.4 27.1 34.4 23.5 22.4 25.9 24.1 23.7 25.8 36.7 36.2 34.3 37.4
25 PI15-Hs00210658_m1 Core down 32.0 30.7 30.3 23.1 28.3 26.5 24.6 32.3 27.0 30.9 29.6 25.0 24.5 24.8 25.4 26.0 32.3 27.4 29.1 30.0 26.6 32.9 32.0 30.5
26 EPDR1-Hs00378148_m1 Core up 29.0 28.3 28.8 26.6 27.3 28.8 26.9 29.5 28.9 29.0 30.8 29.0 30.0 30.0 26.0 25.2 28.6 28.8 28.7 28.2 26.6 28.1 28.8 28.8
27 DTX4-Hs00392288_m1 Core down 33.2 31.1 31.3 28.3 31.4 28.3 27.2 33.6 27.4 30.2 32.9 26.5 25.0 26.7 33.3 34.4 30.8 30.6 31.3 33.3 29.5 32.3 28.4 28.0
28 CXXC4-Hs00228693_m1 Core down 31.3 31.2 30.8 34.2 36.8 30.2 29.6 32.1 29.7 33.1 28.3 25.9 25.7 27.9 27.7 24.9 27.4 26.5 25.5 27.5 36.7 36.2 29.3 30.3
29 IRX2-Hs01383002_m1 Core down 30.4 30.9 30.7 29.3 28.9 30.8 30.1 29.7 30.3 31.1 30.7 26.6 26.6 26.3 28.9 27.3 37.4 36.8 36.3 37.7 29.9 29.5 37.9 37.4
30 MAP6-Hs01023152_s1 Core down 28.0 25.1 25.4 25.9 27.4 24.1 22.0 27.5 24.1 24.7 28.1 26.9 25.8 28.0 25.9 24.1 27.4 26.7 26.8 27.2 28.3 29.8 29.2 29.1
31 SIX3-Hs00193667_m1 Core down 37.4 37.4 37.1 37.1 36.8 36.4 34.8 37.5 35.4 36.9 37.0 25.5 24.0 25.0 36.1 32.6 32.5 33.6 29.8 32.3 32.3 32.9 35.1 34.7
32 NKX2-2-Hs00159616_m1 Marker 37.4 37.4 37.1 37.1 36.8 32.6 33.8 37.5 35.4 36.9 37.0 28.6 30.8 29.6 25.0 23.7 27.2 26.1 28.2 30.0 26.5 26.4 28.9 28.2
33 S100A6-Hs00170953_m1 Core up 20.0 20.4 20.8 20.9 18.9 20.0 20.8 19.5 21.7 20.5 21.9 21.1 25.7 23.0 18.8 17.3 21.7 22.7 21.6 19.8 19.5 17.8 19.7 19.2
34 MMP17-Hs01108847_m1 Core up 28.3 28.7 28.6 28.4 27.5 29.7 29.1 28.0 29.3 30.0 28.6 32.3 30.3 29.9 25.7 27.4 30.4 29.8 29.1 30.8 27.4 26.4 28.9 28.5
35 FAM38B-Hs00926225_m1 Core down 37.4 37.4 37.1 37.1 36.8 36.4 37.2 37.5 35.4 36.9 37.0 24.3 27.0 26.1 36.1 34.4 37.4 36.8 36.3 37.7 24.4 24.5 29.3 29.1
36 OLIG2-Hs00300164_s1 Marker 30.2 33.1 33.2 30.1 30.0 28.3 28.4 32.5 34.3 35.4 29.2 26.1 28.1 27.8 23.4 23.9 22.2 22.0 21.5 23.4 30.2 32.0 31.4 36.4
37 PMEPA1-Hs00375306_m1 Core up 26.0 24.0 24.1 24.8 24.9 25.2 26.0 26.1 26.5 25.9 27.6 26.5 29.1 27.2 25.5 25.3 27.7 27.7 27.5 27.3 25.9 24.3 24.0 24.6
38 HOXD10-Hs00157974_m1 Core up 37.4 37.4 37.1 37.1 36.8 32.5 32.4 37.5 35.4 36.9 37.0 37.5 37.1 37.9 29.1 27.4 27.4 27.7 28.1 27.4 28.0 28.1 28.7 28.7
39 NDN-Hs00267349_s1 Core down 25.1 25.0 25.2 24.2 24.1 24.7 23.4 25.0 25.3 25.1 25.4 23.8 23.9 24.7 25.0 23.1 25.0 25.4 25.9 26.1 23.4 24.1 24.7 24.3
40 FOXG1-Hs01850784_s1 Core up 37.4 37.4 37.1 33.7 34.7 25.3 25.3 37.5 31.1 35.8 35.3 27.8 27.6 28.8 25.0 23.5 23.9 23.9 21.4 21.4 24.0 24.4 24.6 24.4
41 MN1-Hs00159202_m1 Core down 32.3 31.8 31.5 29.4 30.3 31.2 27.8 33.6 31.8 34.1 31.0 26.3 25.9 27.0 28.7 28.5 30.1 28.2 28.8 28.5 34.0 32.3 33.4 33.3
42 NDUFB10-Hs00605903_m1 Norm 25.1 25.5 26.0 25.6 25.0 25.8 25.0 25.5 26.0 25.8 25.4 25.6 26.0 26.0 24.6 22.4 25.5 25.5 26.4 26.2 25.2 24.4 25.0 25.4
43 HMGA2-Hs00171569_m1 Core down 31.8 28.3 28.5 33.6 30.3 29.1 29.7 30.3 28.2 28.8 29.0 27.3 27.1 26.8 31.0 29.5 31.3 30.3 28.9 28.9 34.1 30.5 30.4 31.0
44 MMRN1-Hs00201182_m1 Core down 37.4 37.4 37.1 37.1 36.8 36.4 37.2 37.5 35.4 36.9 37.0 26.4 27.3 28.6 36.1 34.4 37.0 36.2 36.3 36.7 36.7 36.1 33.2 34.3
45 RAB6B-Hs00981572_m1 Core down 34.8 32.4 32.3 32.9 35.1 31.7 29.7 34.6 31.1 32.4 30.4 28.3 28.6 29.0 28.5 28.1 27.6 27.7 27.3 28.4 29.3 29.4 30.2 29.3
46 NTRK2-Hs00178811_m1 Core up 29.3 30.9 31.1 23.4 24.7 25.5 23.1 29.7 28.5 30.9 29.0 29.0 26.9 28.6 21.5 20.8 29.5 24.8 26.9 27.5 22.4 24.2 25.2 24.0
47 KALRN-Hs00610179_m1 Core down 29.9 29.5 30.1 28.1 29.5 30.3 29.0 30.0 28.4 30.0 28.3 26.5 25.9 26.5 27.7 28.5 28.7 27.8 27.4 28.2 26.8 28.9 30.8 30.6
48 CD74-Hs00269961_m1 Core up 27.6 27.7 27.5 28.0 28.7 27.6 24.2 27.8 25.7 28.8 22.2 27.4 30.3 26.3 20.8 21.6 25.7 23.7 25.7 25.5 21.6 26.3 27.8 28.3
261