Transcriptional Characterization of Glioma Neural Stem Cells Diva ...

A.3 Quantitative RT-PCR Appendix
Well Applied Biosystems assay ID Gene G19 G19 G21 G21 G23 G23 G24 G24 G25 G25 G26 G26 G30 G30 G31 G31 G32 G32 G144 G144 G166 G166 G179 G179
Category A B A B A B A B A B A B A B A B A B A B A B A B
1 MYL9-Hs00382913_m1 Core down 34.0 36.1 33.8 33.1 29.1 31.9 27.7 27.4 30.7 29.9 28.8 29.9 34.1 33.6 35.1 34.8 31.1 32.2 34.2 33.9 28.3 28.6 31.9 31.4
2 CHCHD10-Hs01369775_g1 Core up 26.2 27.2 26.9 27.3 25.8 25.9 26.1 26.0 26.5 25.6 25.2 26.3 26.6 26.7 26.5 26.5 27.0 26.6 27.6 26.7 25.3 24.8 24.6 24.0
3 RGS5-Hs00186212_m1 Core down 31.0 31.6 32.1 32.9 30.0 29.9 31.3 31.5 30.3 30.0 30.2 32.3 30.0 30.1 30.0 30.5 30.1 30.6 36.2 33.0 30.4 31.4 31.1 30.1
4 ST6GALNAC5-
Core down 29.7 31.1 25.5 26.1 32.9 30.3 33.1 40.0 35.9 34.3 29.9 31.4 33.5 33.7 27.1 27.0 28.8 28.6 36.0 35.9 30.9 33.5 34.5 33.2
Hs00229612_m1
5 CEBPB-Hs00270923_s1 Core up 30.6 30.4 30.0 30.7 28.2 27.8 29.9 30.2 29.8 29.5 29.7 30.5 29.9 29.7 29.1 29.2 29.7 29.9 32.2 31.5 28.4 28.7 28.1 28.2
6 C5orf13-Hs00854282_g1 Core down 29.1 28.1 27.2 27.8 28.2 25.2 21.6 24.4 26.3 26.7 40.0 29.4 26.4 25.0 40.0 26.3 40.0 27.3 26.9 40.0 26.1 28.0 28.7 28.2
7 PDGFRA-Hs00998026_m1 Marker 24.7 25.0 22.5 22.3 25.3 27.3 24.9 24.4 26.5 26.0 23.8 26.9 23.6 23.6 27.5 27.8 27.3 28.9 26.7 26.2 25.2 28.6 26.5 25.9
8 CCND2-Hs00922419_g1 Core down 35.0 30.5 21.6 22.0 27.9 27.4 30.0 32.4 27.4 26.8 24.5 26.0 22.1 22.0 20.7 20.9 20.6 21.2 25.1 25.3 32.4 32.0 32.4 31.6
9 NKX2-1-Hs00163037_m1 Core down 31.1 31.5 35.3 35.3 40.0 34.8 35.8 40.0 40.0 40.0 40.0 40.0 40.0 40.0 31.2 31.7 32.2 31.0 34.7 36.9 40.0 40.0 40.0 40.0
10 CTSC-Hs00175188_m1 Core up 21.9 23.2 24.2 25.5 21.6 20.7 26.0 25.5 22.5 22.2 20.5 22.1 24.0 23.6 24.4 24.5 24.3 24.5 23.9 22.8 21.2 22.8 24.3 23.1
11 18S-Hs99999901_s1 Norm 12.9 13.1 12.0 12.4 11.9 12.2 11.7 13.0 12.3 11.9 11.7 12.2 12.1 11.9 11.4 11.9 11.6 12.0 14.5 13.8 12.0 13.8 11.8 11.4
12 DNER-Hs00294564_m1 Core down 31.4 28.4 27.3 26.4 32.7 32.4 27.7 27.2 26.6 26.7 25.3 27.9 24.2 24.0 23.9 24.5 25.0 26.1 31.3 28.6 27.0 30.3 27.9 27.0
13 LMO4-Hs01086790_m1 Core up 24.5 25.3 21.9 22.3 23.4 23.6 24.4 24.1 24.4 23.8 23.3 25.4 22.8 22.5 22.4 22.6 22.9 22.9 25.7 24.7 23.7 24.1 24.4 23.2
14 PLCH1-Hs00392783_m1 Core down 26.0 26.3 27.1 27.7 27.0 28.7 28.1 28.5 31.6 30.5 29.4 29.9 29.6 29.3 27.3 27.4 27.5 27.0 32.5 31.5 28.8 29.6 31.2 31.0
15 SPARCL1-Hs00949886_m1 Core down 26.9 25.6 26.4 27.4 26.2 28.9 25.1 24.0 27.5 27.3 24.8 29.3 24.3 24.0 26.5 26.4 27.0 28.9 31.2 31.3 27.9 30.5 26.3 25.2
16 SYNM-Hs00322391_m1 Core down 34.0 33.4 27.9 28.2 27.7 27.6 27.2 28.3 36.0 40.0 29.4 29.3 40.0 36.1 31.9 33.3 31.7 32.0 40.0 40.0 36.8 40.0 29.6 29.0
17 FBLN2-Hs00157482_m1 Core down 40.0 35.5 31.0 31.9 30.1 30.7 36.7 35.9 32.8 33.9 34.3 36.2 34.1 34.6 34.3 34.5 33.9 35.4 34.3 35.4 36.5 34.7 28.7 28.6
18 SALL2-Hs00413788_m1 Core down 29.9 28.1 26.6 26.3 34.3 36.9 35.1 33.2 32.0 31.0 29.5 33.1 26.3 26.2 26.6 26.5 26.8 27.2 30.2 28.6 40.0 40.0 33.9 33.9
19 TUSC3-Hs00185147_m1 Core down 23.2 23.5 40.0 40.0 24.4 25.0 34.1 32.9 37.0 35.0 40.0 40.0 24.9 24.9 40.0 40.0 30.0 30.2 40.0 40.0 32.0 28.8 26.5 25.3
20 GPR158-Hs00393109_m1 Core down 30.6 30.0 27.9 28.2 40.0 40.0 32.3 32.7 31.1 31.6 28.2 30.4 30.5 31.2 28.7 29.3 29.1 29.2 28.2 28.9 31.4 33.3 31.0 30.9
21 PEG3-Hs00377844_m1 Core down 28.9 28.9 23.6 24.9 32.9 33.9 27.8 28.5 27.3 27.3 27.6 30.1 25.2 25.6 40.0 40.0 29.1 32.0 29.5 28.7 29.8 31.4 40.0 40.0
22 FUT8-Hs00189535_m1 Core up 27.4 28.2 25.1 25.1 27.0 27.2 27.1 27.3 26.0 26.0 27.3 28.5 26.2 26.3 26.5 26.2 26.1 26.5 28.3 27.4 25.0 26.0 27.3 26.2
23 HLA-DRA-Hs00219575_m1 Core up 27.0 26.1 33.4 35.0 35.3 40.0 27.9 27.7 22.4 22.9 23.7 24.5 24.0 23.9 33.7 32.0 28.0 30.5 24.4 22.8 28.3 26.6 22.2 21.1
24 ASCL1-Hs00269932_m1 Marker 36.9 30.7 23.1 23.4 28.7 30.4 29.4 33.9 35.4 40.0 36.4 40.0 23.4 23.4 21.9 22.5 22.2 22.6 28.1 26.5 35.1 40.0 31.6 31.3
25 PI15-Hs00210658_m1 Core down 28.9 30.8 32.3 28.9 30.6 32.2 28.9 26.6 27.5 27.6 27.8 26.9 31.1 30.6 36.0 37.0 27.7 34.6 32.6 34.1 27.4 30.0 28.2 26.7
26 EPDR1-Hs00378148_m1 Core up 27.6 29.2 30.2 30.8 28.5 29.2 27.6 28.2 26.9 25.8 26.9 28.0 27.9 27.8 29.2 29.1 29.1 28.9 30.8 29.8 27.1 26.8 29.5 28.6
27 DTX4-Hs00392288_m1 Core down 27.8 27.7 28.5 28.0 32.8 33.8 30.4 30.5 30.0 29.4 34.5 34.2 29.8 29.5 26.4 26.4 26.5 27.6 30.4 30.7 31.0 31.7 28.5 28.1
28 CXXC4-Hs00228693_m1 Core down 29.7 28.2 24.3 24.7 29.1 30.9 28.2 29.9 36.9 40.0 34.9 40.0 26.9 26.7 25.4 26.1 25.4 26.9 30.7 29.3 30.4 32.8 36.9 35.0
29 IRX2-Hs01383002_m1 Core down 40.0 40.0 40.0 40.0 28.4 28.7 36.4 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 37.1 34.9 40.0 40.0 40.0 40.0 30.5 30.4
30 MAP6-Hs01023152_s1 Core down 27.9 28.3 26.0 26.7 28.3 29.6 30.9 29.7 30.4 30.8 32.4 32.6 28.4 28.2 29.1 29.4 28.6 29.8 33.7 31.8 30.7 33.7 27.5 26.5
31 SIX3-Hs00193667_m1 Core down 33.0 34.0 40.0 40.0 29.9 30.5 34.5 34.8 36.0 35.9 40.0 40.0 35.1 36.4 34.7 36.1 40.0 34.9 31.6 30.8 33.7 33.1 40.0 40.0
32 NKX2-2-Hs00159616_m1 Marker 28.7 28.4 26.5 27.8 27.5 27.9 26.4 26.8 25.5 24.8 25.8 27.4 24.9 24.6 25.0 25.3 24.8 24.5 27.1 25.9 28.4 28.8 31.0 31.0
33 S100A6-Hs00170953_m1 Core up 18.5 20.1 29.2 27.3 18.3 17.8 19.9 20.0 20.3 19.2 21.4 21.2 21.1 20.9 23.0 22.9 22.7 21.8 24.0 23.8 17.5 17.9 19.3 18.3
34 MMP17-Hs01108847_m1 Core up 28.4 29.6 30.3 30.1 28.6 27.7 28.9 28.8 25.1 24.9 30.1 31.3 26.2 25.9 29.4 28.8 29.2 29.2 29.2 26.4 26.6 26.4 28.6 28.4
35 FAM38B-Hs00926225_m1 Core down 29.0 29.4 40.0 40.0 36.9 36.4 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 32.7 31.6
36 OLIG2-Hs00300164_s1 Marker 34.9 29.1 22.1 22.8 26.8 27.5 25.4 26.1 23.1 22.9 23.1 24.9 21.8 21.2 22.6 22.8 22.6 22.7 24.8 23.6 31.0 33.2 30.5 30.5
37 PMEPA1-Hs00375306_m1 Core up 24.1 25.1 27.9 27.6 25.5 26.4 22.4 23.0 25.4 24.3 26.7 27.3 25.2 24.6 27.1 26.9 27.4 26.9 27.3 27.7 22.6 25.0 26.0 25.7
38 HOXD10-Hs00157974_m1 Core up 28.5 28.5 40.0 40.0 27.7 28.6 29.4 29.5 29.5 28.9 29.5 31.2 28.9 28.6 28.8 28.9 28.8 29.0 30.7 28.8 26.5 27.0 27.9 27.2
39 NDN-Hs00267349_s1 Core down 23.8 24.2 29.4 30.1 40.0 40.0 25.9 25.8 25.9 25.7 27.1 27.1 36.9 40.0 25.4 25.1 25.5 25.0 28.6 27.0 35.7 40.0 40.0 35.2
40 FOXG1-Hs01850784_s1 Core up 24.4 24.2 22.9 23.0 24.6 25.3 24.3 25.3 25.5 24.8 26.3 26.3 23.5 23.5 21.4 21.6 21.4 21.8 26.3 24.7 23.8 25.5 21.8 21.3
41 MN1-Hs00159202_m1 Core down 32.2 34.9 28.0 27.9 31.8 33.4 26.4 27.3 30.5 28.9 33.7 35.5 28.0 27.1 26.5 27.2 26.8 26.4 31.6 30.1 34.7 35.0 31.5 30.8
42 NDUFB10-Hs00605903_m1 Norm 25.0 25.6 24.5 25.2 25.3 25.3 25.1 25.7 26.0 25.4 24.7 25.9 25.3 24.9 25.2 25.5 25.4 25.2 28.5 26.6 24.5 25.0 26.4 25.3
43 HMGA2-Hs00171569_m1 Core down 29.9 32.0 33.0 33.2 27.5 27.2 35.4 35.9 32.4 31.8 32.1 33.6 32.4 31.5 33.1 32.8 32.6 32.3 35.8 33.8 31.3 32.1 29.4 28.7
44 MMRN1-Hs00201182_m1 Core down 33.7 31.6 40.0 40.0 33.0 34.6 40.0 40.0 40.0 40.0 32.0 31.5 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 33.7 35.0 33.6 32.2
45 RAB6B-Hs00981572_m1 Core down 28.9 30.7 29.8 29.3 33.2 33.0 27.9 28.0 29.5 29.0 30.7 32.4 28.0 27.4 26.6 26.8 26.7 27.1 31.7 30.2 29.6 30.5 31.0 29.9
46 NTRK2-Hs00178811_m1 Core up 24.0 24.6 29.5 29.7 31.6 32.6 33.2 33.1 23.4 23.4 23.3 26.3 24.3 24.0 34.6 37.0 27.5 31.8 26.4 25.1 22.5 24.8 28.1 27.4
47 KALRN-Hs00610179_m1 Core down 30.5 30.6 27.5 27.4 30.3 31.8 28.5 28.3 30.7 30.1 30.6 31.1 27.2 27.5 27.4 27.5 26.7 27.4 32.2 30.8 27.9 30.9 31.2 31.0
48 CD74-Hs00269961_m1 Core up 27.5 26.4 32.1 35.1 32.7 34.2 27.9 28.2 22.2 23.0 24.2 25.6 24.7 24.4 36.5 33.5 28.3 30.3 24.9 24.7 28.7 26.6 22.3 22.0
49 LUM-Hs00158940_m1 Core down 30.1 31.9 40.0 40.0 23.4 26.0 32.8 27.5 40.0 40.0 31.4 31.2 40.0 40.0 32.3 31.2 34.9 40.0 40.0 40.0 28.8 27.5 30.4 29.1
50 NELL2-Hs00196254_m1 Core down 33.1 33.2 33.2 29.0 31.6 32.1 29.8 29.6 36.9 37.0 32.5 34.4 30.8 31.4 27.0 27.4 28.2 29.1 30.1 28.0 29.1 31.8 32.2 31.1
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