Transcriptional Characterization of Glioma Neural Stem Cells Diva ...

A.3 Quantitative RT-PCR Appendix
Well Applied Biosystems assay ID Gene CB130 CB130 CB130 CB152 CB152 CB152 CB152 CB171 CB171 CB171 CB192 CB541 CB660 CB660 G2 G2 G7 G7 G7 G7 G9 G9 G14 G14
Category A B C A B C D A B C A B A B A B C D A B A B
49 LUM-Hs00158940_m1 Core down 29.4 28.9 29.2 30.3 31.9 36.9 32.0 31.6 33.4 32.8 29.9 25.5 24.6 24.6 40.0 40.0 32.5 29.0 33.0 31.0 23.0 27.3 29.6 29.7
50 NELL2-Hs00196254_m1 Core down 24.5 24.3 24.7 28.0 29.3 29.1 24.9 24.3 25.6 25.3 25.3 24.6 23.8 25.0 30.5 30.9 30.2 30.7 31.7 30.7 28.7 30.0 34.2 34.0
51 MAP6-Hs01929835_s1 Core down 27.0 25.3 26.1 25.6 26.6 25.0 22.4 26.5 26.0 25.1 27.4 26.1 25.6 27.9 25.8 25.9 26.8 26.4 28.5 27.7 27.3 29.3 26.3 26.5
52 PDE1C-Hs01095694_m1 Core up 29.9 32.4 32.9 27.0 27.9 29.0 28.0 29.2 31.4 30.8 30.4 26.2 27.9 25.9 26.4 28.0 24.3 24.6 25.5 23.3 23.8 27.1 24.2 24.4
53 SULF2-Hs00378697_m1 Core up 28.5 29.7 30.1 25.9 26.8 29.1 25.4 27.9 30.9 31.3 26.8 26.4 27.8 26.7 23.0 23.9 23.3 23.1 25.3 24.4 24.0 23.7 23.9 23.8
54 TUBB-Hs00962420_g1 Norm 20.0 21.3 21.5 21.2 20.6 23.1 22.7 19.8 24.0 22.2 21.3 20.4 21.0 20.2 21.5 23.0 20.8 21.1 23.1 20.6 21.7 21.5 20.5 20.8
55 LMO2-Hs00277106_m1 Core down 36.8 37.1 33.7 35.3 34.1 30.0 29.1 36.0 34.9 33.1 30.4 26.1 25.7 26.3 26.8 27.2 25.4 26.6 27.1 26.8 28.1 30.8 31.7 31.9
56 MAN1C1-Hs00220595_m1 Core up 27.8 26.9 27.0 25.9 29.0 27.8 25.7 27.2 27.2 27.0 27.1 31.1 29.0 28.9 26.8 29.0 26.0 26.8 27.5 26.4 25.2 27.4 27.6 27.4
57 TAGLN-Hs00162558_m1 Core down 21.2 18.8 18.5 20.5 21.9 20.2 21.9 22.0 20.7 20.0 21.8 21.2 23.6 21.8 27.4 29.7 40.0 36.9 37.2 29.3 21.6 22.1 28.6 28.9
58 RTN1-Hs00382515_m1 Core down 26.4 23.0 23.2 29.7 28.8 28.9 25.9 27.2 27.6 26.9 23.8 23.8 25.5 25.3 26.1 26.1 23.4 24.4 26.3 24.2 26.1 27.3 24.7 25.2
59 MAF-Hs00193519_m1 Core down 26.5 25.9 26.7 26.4 25.8 27.4 26.0 26.4 29.7 27.6 27.1 25.2 26.4 26.0 27.4 27.0 27.8 29.4 27.9 25.2 30.1 30.5 27.9 28.2
60 NPTX2-Hs00383983_m1 Core down 35.2 30.6 30.5 30.5 26.8 32.8 29.8 40.0 36.9 35.3 33.6 22.9 25.5 23.6 40.0 40.0 26.2 29.4 31.4 28.5 31.1 26.8 29.6 29.2
61 EDA2R-Hs00939736_m1 Core down 27.9 28.0 28.0 27.6 27.2 28.9 26.8 27.5 29.6 28.2 28.5 26.6 28.4 27.0 27.8 29.2 30.3 34.3 33.5 30.9 28.2 28.9 26.2 26.7
62 SOX10-Hs00366918_m1 Marker 40.0 40.0 40.0 40.0 40.0 35.2 32.8 40.0 40.0 40.0 40.0 40.0 35.1 40.0 27.9 28.0 40.0 37.0 36.0 36.0 40.0 40.0 40.0 40.0
63 SEMA6A-Hs00221174_m1 Core down 25.0 25.9 25.9 24.3 25.2 26.2 24.3 24.6 26.8 26.4 22.7 22.9 23.0 23.3 25.6 26.4 24.9 25.2 26.7 26.3 26.7 30.2 26.2 27.3
64 DDIT3-Hs00358796_g1 Core up 28.0 29.0 29.2 28.4 27.6 27.9 27.3 28.5 30.3 28.6 28.7 27.6 27.9 27.5 27.4 27.2 25.9 25.9 28.8 26.2 26.6 27.1 26.4 26.9
65 CA12-Hs01080909_m1 Core down 30.1 32.2 32.6 30.9 28.3 29.5 31.0 30.9 32.6 31.5 21.5 22.1 23.4 22.5 25.4 26.1 23.4 23.1 24.9 22.3 24.1 26.4 30.4 30.8
66 C9orf125-Hs00260558_m1 Core down 32.9 32.2 32.8 36.7 40.0 34.6 34.8 33.7 35.4 36.3 29.4 28.5 28.1 29.0 28.0 30.2 29.0 28.5 30.2 29.4 28.3 28.2 30.4 31.0
67 SDC2-Hs00299807_m1 Core down 24.7 24.5 24.7 24.1 24.5 25.0 23.3 25.3 26.4 25.2 25.5 25.1 25.8 25.5 27.4 27.0 26.6 28.4 28.3 26.7 26.4 27.9 26.1 26.5
68 SLIT2-Hs00191193_m1 Core down 29.3 33.1 32.9 28.9 28.7 31.4 29.3 29.8 33.0 32.9 31.8 24.0 24.2 24.4 40.0 36.4 27.8 27.6 31.9 30.7 26.4 28.3 25.1 25.5
69 MT2A-Hs02379661_g1 Core up 25.2 23.6 24.2 22.9 22.4 20.9 20.4 25.5 25.3 23.9 23.9 23.3 24.4 24.2 22.5 22.2 20.3 21.4 22.4 20.0 19.1 19.0 22.5 22.3
70 IL17RD-Hs00296982_m1 Core down 26.8 25.7 25.7 25.8 26.9 27.1 26.2 25.8 28.3 26.9 26.3 23.9 24.4 23.7 27.4 28.7 27.7 27.0 27.6 26.9 24.3 24.8 25.9 26.0
71 PDZRN3-Hs00392900_m1 Core down 29.0 28.9 29.0 27.2 28.8 29.6 26.6 28.4 27.7 28.8 28.4 26.4 26.7 26.8 29.8 32.4 27.1 29.1 29.3 29.6 27.9 30.9 29.3 29.6
72 LGALS3-Hs00173587_m1 Core up 25.7 24.5 25.4 24.8 24.0 24.5 23.0 25.6 26.6 25.1 26.5 25.8 25.6 25.0 25.0 24.0 26.4 27.7 26.2 25.1 22.7 23.5 25.5 25.3
73 FAM69A-Hs00961685_m1 Core up 25.3 25.0 25.2 23.8 24.6 26.6 23.5 24.8 26.5 26.3 26.4 27.6 29.7 28.0 25.6 26.0 25.7 26.5 27.3 25.9 24.9 25.9 25.3 25.3
74 TERT-Hs00972656_m1 Marker 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 36.7 36.5 35.5 33.6 34.4 32.4 32.2 32.7 32.0 33.4 33.4 31.0 34.0
75 PLS3-Hs00418605_g1 Core up 24.6 27.6 27.9 25.0 24.1 26.7 26.4 24.4 29.7 28.9 22.9 26.1 25.6 24.1 23.4 24.4 25.3 26.6 26.9 24.2 22.5 23.1 21.8 22.0
76 PTEN-Hs02621230_s1 Core down 30.0 27.4 27.6 29.4 30.5 28.1 26.4 28.8 28.3 27.5 29.5 27.6 27.6 27.3 40.0 40.0 29.9 30.9 27.9 27.3 40.0 40.0 27.8 28.0
77 SALL2-Hs00826674_m1 Core down 34.4 31.7 31.9 32.2 34.0 33.1 29.7 34.8 32.6 33.0 34.8 32.5 32.4 32.6 31.5 32.2 30.8 31.4 33.2 28.7 33.1 34.9 34.1 33.9
78 KCTD12-Hs00540818_s1 Core down 30.6 30.2 30.3 33.3 33.9 32.7 33.2 29.7 29.7 29.8 26.5 25.3 27.3 26.2 27.8 30.0 30.8 32.4 35.5 32.1 27.2 29.7 33.6 32.4
79 TES-Hs00210319_m1 Core down 25.7 25.6 26.1 27.7 27.3 28.9 28.4 25.7 29.1 26.9 32.6 25.1 26.1 25.6 40.0 36.6 40.0 40.0 40.0 37.0 33.4 40.0 35.9 40.0
80 SOX2-Hs01053049_s1 Marker 25.2 21.3 21.7 24.1 26.2 22.9 20.4 24.3 22.5 22.0 24.4 23.1 23.4 23.5 24.7 24.9 23.0 23.4 22.6 21.7 25.3 27.9 23.7 23.8
81 LPAR6-Hs00271758_s1 Core down 30.9 29.0 29.4 34.3 34.3 32.2 30.7 31.2 30.8 30.1 29.8 27.9 28.5 28.5 31.9 31.9 28.1 29.8 29.4 27.8 27.7 29.6 29.2 29.8
82 ODZ2-Hs00393060_m1 Core down 28.3 27.3 27.5 26.7 28.0 28.1 26.8 28.8 29.9 29.4 25.7 26.9 25.0 24.7 30.8 31.6 24.7 26.4 27.8 26.1 27.8 30.1 25.4 25.8
83 NNMT-Hs00196287_m1 Core up 31.0 27.5 27.9 28.8 32.2 30.1 29.6 32.0 30.9 28.5 29.9 27.7 29.4 27.9 30.9 32.6 23.7 26.1 24.5 22.2 22.3 23.7 28.9 27.0
84 CACNG8-Hs01100182_m1 Core down 40.0 33.0 32.7 32.6 40.0 33.7 34.5 33.6 34.8 35.3 28.7 27.3 26.1 27.3 29.7 30.4 26.8 28.7 30.1 28.8 29.6 32.4 32.0 32.4
85 PRSS12-Hs00186221_m1 Core up 29.9 34.6 34.8 32.1 30.8 34.2 33.4 29.7 33.4 33.8 40.0 29.9 29.6 28.0 27.4 29.4 28.5 30.2 34.2 33.1 25.6 26.8 23.8 24.3
86 FOXJ1-Hs00230964_m1 Core down 35.7 32.9 32.7 26.7 33.7 30.5 26.3 34.8 30.2 33.4 35.5 30.5 29.4 32.2 30.0 31.3 31.2 32.5 31.3 31.0 37.0 40.0 33.0 33.1
87 NTN1-Hs00180355_m1 Core down 30.2 29.3 29.3 26.4 30.4 27.7 25.9 29.5 28.6 29.0 26.9 25.2 25.8 25.5 29.6 30.9 28.9 28.9 28.7 29.5 33.4 32.9 27.5 29.1
88 LMO3-Hs00375237_m1 Core down 29.3 28.5 29.0 34.5 40.0 35.4 30.7 29.6 29.0 30.4 33.9 28.7 25.5 28.4 40.0 40.0 31.2 35.2 28.0 27.9 35.6 36.9 40.0 40.0
89 CHI3L1-Hs00609691_m1 Marker 34.1 31.7 32.4 34.1 35.5 31.4 33.3 40.0 36.0 34.1 32.0 31.8 30.8 29.8 28.7 32.2 22.4 27.3 29.4 26.4 23.0 25.4 30.0 31.1
90 CD9-Hs01124025_g1 Core up 27.5 30.4 30.9 24.9 24.3 26.1 24.1 27.7 29.8 29.9 27.1 27.3 28.0 26.7 21.1 22.2 22.4 22.4 22.8 21.2 24.1 23.8 22.9 23.4
91 ADD2-Hs00242289_m1 Core up 25.9 27.4 27.8 27.1 27.3 29.2 27.8 24.9 29.4 28.8 28.1 37.0 30.3 31.8 27.2 28.9 24.6 25.0 27.7 26.7 28.2 27.9 26.3 26.6
92 MYL9-Hs00697086_m1 Core down 25.4 24.2 24.8 20.9 22.6 24.2 23.6 26.5 30.1 28.8 24.1 22.5 25.5 23.7 31.7 35.8 28.4 33.2 28.6 25.9 22.3 24.2 32.4 33.0
93 LYST-Hs00179814_m1 Core up 31.0 30.1 30.3 30.6 30.6 31.4 29.3 30.3 31.7 31.5 31.1 30.7 32.7 31.5 28.2 28.7 28.4 28.7 30.5 28.5 28.9 30.0 29.1 29.9
94 LAMA2-Hs00166308_m1 Core down 35.3 40.0 37.0 40.0 40.0 40.0 40.0 40.0 40.0 40.0 31.1 28.6 26.7 28.7 32.0 35.2 27.7 32.8 27.9 25.2 25.8 29.3 30.6 30.4
95 PLA2G4A-Hs00233352_m1 Core up 29.2 33.2 33.1 36.0 40.0 33.0 33.1 29.2 33.6 31.8 29.9 34.9 32.1 32.4 29.0 30.2 28.6 29.6 31.0 30.7 29.3 28.9 28.4 28.5
96 BACE2-Hs00273238_m1 Core up 26.5 26.7 27.1 26.7 26.2 28.3 26.8 26.2 29.1 28.2 34.0 31.7 31.1 30.5 26.0 26.5 26.6 26.7 28.8 26.3 26.3 25.5 24.5 25.0
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