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Table 4.1 Cont’d<br />

Lesson<br />

5. Light independent<br />

(dark)<br />

phase<br />

Periods<br />

(min)<br />

3<br />

(150)<br />

Learning outcomes Concepts<br />

Students would be<br />

able to…<br />

1. Explain how the<br />

scientists<br />

worked for<br />

photosynthesis.<br />

2. Explain the<br />

process of the<br />

dark phase.<br />

3. Distinguish<br />

between the<br />

dark phase of c3<br />

pl<strong>an</strong>ts <strong>an</strong>d c4<br />

pl<strong>an</strong>ts.<br />

4. Evaluate own<br />

learning about<br />

the dark phase.<br />

10. NADP+ is reduced to NADPH on the stromal side<br />

of the membr<strong>an</strong>e via the action of ferredoxin (Fd)<br />

<strong>an</strong>d the flavoprotein ferredoxin-NADP reductase<br />

(FP).<br />

11. All the electron carriers are known to function in<br />

electron flow from water (H2O) to NADP+.<br />

12. PS-II produces <strong>an</strong> oxid<strong>an</strong>t that c<strong>an</strong> oxidize the<br />

cytochrom b6f complex.<br />

13. The tr<strong>an</strong>sfer of electrons (e-) <strong>an</strong>d protons (H+) in<br />

thylakoid membr<strong>an</strong>e is carried out by protein<br />

complexes--PS-II, the cytochrom b6f, PS-I, <strong>an</strong>d the<br />

ATP synthease.<br />

1. The light independent reaction occurs in stroma, <strong>an</strong>d<br />

is carbon dioxide fixation (called Calvin cycle).<br />

2. The Calvin cycle is fixing CO2, <strong>an</strong>d using ATP <strong>an</strong>d<br />

NADPH generated by the light dependent reactions,<br />

to produce carbohydrate e.g. starch, sugar.<br />

3. The Calvin cycle proceeds in 3 stages: 1)<br />

carboxylation of CO2 acceptor, 2) reduction of PGA<br />

<strong>an</strong>d 3) regeneration of the CO2 receptor.<br />

4. Firstly, CO2 <strong>an</strong>d H2O from environment are<br />

enzymatically combined with a five-carbon acceptor<br />

molecule (RuBP).<br />

5. RuBP generates two molecules of a three-carbon<br />

intermediate (PGA).<br />

6. Secondarily, the intermediate (PGA) is reduced to<br />

carbohydrate (PGAL), sugar, a six-carbon molecule,<br />

by use of the ATP <strong>an</strong>d NADPH generated<br />

photochemically.<br />

Learning<br />

Activities<br />

1. Role play<br />

based on<br />

historical<br />

story<br />

2. Cooperativ<br />

e learning<br />

3. Classroom<br />

discussion<br />

4. Concept<br />

mapping<br />

Materials Assessment<br />

1. The dialogue<br />

of role play<br />

2. The student<br />

booklet: The<br />

long journey<br />

of the light<br />

independent<br />

phase<br />

3. The work<br />

sheet:<br />

Photosynthesi<br />

s of C3-<strong>an</strong>d<br />

C4-pl<strong>an</strong>t<br />

119<br />

1. Use the role play<br />

to evaluate the<br />

students’ ideas about<br />

nature of science as<br />

social enterprise.<br />

2. Use the student<br />

booklet: The long<br />

journey of the light<br />

independent phase<br />

to probe the<br />

students’ ideas about<br />

the phase.<br />

119

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