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Identification of the major drivers of 'phenolic' taste in ... - GWRDC

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AWRI: <strong>Identification</strong> Of The Major Drivers Of ‘Phenolic’ Taste In White W<strong>in</strong>es<br />

3 Do Different Phenolic Pr<strong>of</strong>iles Affect<br />

<strong>the</strong> Taste Pr<strong>of</strong>iles <strong>of</strong> White W<strong>in</strong>es?<br />

3.1 Introduction<br />

It has been established <strong>in</strong> an Australian scenario that overall ‘phenolic <strong>taste</strong>’ amongst Australian w<strong>in</strong>es<br />

can be dist<strong>in</strong>guished, at least by highly experienced w<strong>in</strong>e assessors (Chapter 2). However, <strong>the</strong> diversity <strong>of</strong><br />

varieties and w<strong>in</strong>emak<strong>in</strong>g styles is known to result <strong>in</strong> w<strong>in</strong>es with differ<strong>in</strong>g amounts and types <strong>of</strong> phenolics<br />

i.e. different ‘phenolic pr<strong>of</strong>iles’. It rema<strong>in</strong>s to be established whe<strong>the</strong>r, and <strong>in</strong> what ways, <strong>the</strong>se different<br />

w<strong>in</strong>e phenolic pr<strong>of</strong>iles might <strong>in</strong>fluence phenolic <strong>taste</strong>s. In order to study this, whole phenolic fractions <strong>of</strong><br />

differ<strong>in</strong>g phenolic composition were first isolated from commercial w<strong>in</strong>e and characterised by HPLC. The<br />

whole phenolic fractions were <strong>the</strong>n added to two commercial w<strong>in</strong>es after which <strong>the</strong>ir phenolic <strong>taste</strong><br />

pr<strong>of</strong>iles were quantified by a tra<strong>in</strong>ed sensory panel. W<strong>in</strong>es created from a diversity <strong>of</strong> varieties and<br />

w<strong>in</strong>emak<strong>in</strong>g styles <strong>in</strong>herently have a range <strong>of</strong> different basic composition. Alcohol concentration can vary<br />

greatly and can be highly <strong>in</strong>fluential <strong>in</strong> <strong>the</strong> perception <strong>of</strong> phenolic <strong>taste</strong>s (see Chapter 5) and as such, its<br />

<strong>in</strong>fluence was also <strong>in</strong>vestigated as a variable with <strong>the</strong>se whole phenolic fractions.<br />

3.2 Methods<br />

3.2.1 Sample Preparation<br />

A current v<strong>in</strong>tage commercial Riesl<strong>in</strong>g and unwooded Chardonnay were used as base w<strong>in</strong>es. Whole<br />

phenolics were extracted from ano<strong>the</strong>r three w<strong>in</strong>es that were deemed by a panel <strong>of</strong> experienced <strong>taste</strong>rs to<br />

exhibit phenolic <strong>taste</strong>s. They were a McLaren Vale Fiano, a Canberra District Viognier and an Alsatian<br />

Gewurztram<strong>in</strong>er. The whole phenolics from <strong>the</strong>se w<strong>in</strong>es were eluted <strong>of</strong>f Amberlite FPX66 res<strong>in</strong> us<strong>in</strong>g<br />

96% ethanol, <strong>the</strong> ethanol evaporated under vacuum and redissolved <strong>in</strong> water and freeze dried. All samples<br />

<strong>of</strong> whole phenolics were kept at -80°C before use.<br />

Whole phenolics were extracted from a volume <strong>of</strong> w<strong>in</strong>e as described above and <strong>the</strong>n 50% <strong>of</strong> <strong>the</strong> whole<br />

phenolics that were collected were added back to <strong>the</strong> same volume <strong>of</strong> base w<strong>in</strong>e just prior to tast<strong>in</strong>g. An<br />

additional treatment factor <strong>of</strong> an additional 1% ethanol v/v (96%, Tarac Industries, SA) was <strong>in</strong>cluded to<br />

assess <strong>the</strong> effect <strong>of</strong> alcohol concentration on <strong>the</strong> perception <strong>of</strong> phenolic <strong>taste</strong>s. The whole phenolic<br />

fractions were analysed us<strong>in</strong>g <strong>the</strong> HPLC method described <strong>in</strong> Section A.3.<br />

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