Experimentelle Untersuchungen zur Transgenese bei Nutztieren

More documents

Recommendations

Info

1.0 Eigene Publikationen <strong>zur</strong> Thematik dieser Habilitationsschrift 1.1 Charakterisierung primärer Zellen als Donorzellen für den Kerntransfer Anger, M., Kues, W.A., Klima, J., Mielenz, M., Motlik, J., Carnwath, J.W. and Niemann, H. 2003. Cloning and cell cycle-specific regulation of Polo-like kinase in porcine fetal fibroblasts. Molecular Reproduction and Development 65, 245-253. Kues, W. A., Petersen, B., Mysegades, W., Carnwath, J.W. and Niemann, H. 2005. Isolation of fetal stem cells from murine and porcine somatic tissue. Biology of Reproduction 72, 1020-1028 Yadav, P., Kues, W.A., Herrmann, D., Carnwath, J.W., Niemann, H. 2005. Bovine ICMs express the Oct4 ortholog. Molecular Reproduction and Development 72, 182-190. Dieckhoff, B., Karlas, A., Hofmann, A., Kues, W.A., Petersen, B., Pfeifer, A., Niemann, H., Kurth, R., Denner, J. 2007. Inhibition of porcine endogenous retroviruses (PERVs) in primary porcine cells by RNA interference using lentiviral vectors. Archives of Virology 152, 629-34. 1.2 DNA-Mikroinjektion und somatischer Kerntransfer Schätzlein S., Lucas-Hahn, A., Lemme, E., Kues, W.A., Dorsch, M., Manns, M., Niemann, H., Rudolph, K.H. 2004. Telomere length is reset during early embryogenesis. Proceedings of the National Academy of Sciences of the USA 101, 8034-8038. Hölker, M., Petersen, B., Hassel, P., Kues, W.A., Lemme, E., Lucas-Hahn, A., Niemann, H. 2005. Duration of in vitro maturation of recipient oocytes affects blastocyst development of cloned porcine embryos. Cloning and Stem Cells 7, 34-43. Kues, W.A., Schwinzer, R., Wirth, D., Verhoeyen, E., Lemme, E., Herrmann, D., Barg-Kues, B., Hauser, H., Wonigeit, K., Niemann H. 2006. Epigenetic silencing and tissue independent expression of a novel tetracycline inducible system in double-transgenic pigs. FASEB Journal 20, E1-E10, doi: 10.1096/fj.05-5415fje. Deppenmeier, S., Bock, O., Mengel, M., Niemann, H., Kues, W., Lemme, E., Wirth, D, Wonigeit, K., Kreipe, H. 2006. Health status of transgenic pig lines expressing human complement regulator protein CD59. Xenotransplantation 13, 345-356. Hornen, N., Kues, W.A., Carnwath, J.W., Lucas-Hahn, A., Petersen, B., Hassel, P., Niemann, H. 2007. Production of viable pigs from fetal somatic stem cells. Cloning and Stem Cells 9, 364-73. Iqbal, K, Kues, W.A., Niemann, H. 2007. Parent of origin dependent gene-specific knock down in mouse embryos. Biochemical and Biophysical Research Communications 358, 727-732. 4
Dieckhoff, B., Petersen, B., Kues, W.A., Kurth, R., Niemann, H., Denner, J. 2008. Knockdown of porcine endogenous retrovirus (PERV) expression by PERV-specific shRNA in transgenic pigs. Xenotransplantation 15, 36-45. 1.3 Übersichtsartikel: Transgene Nutztiere, Stammzellen und DNA-Array Analysen Niemann, H., und Kues, W.A. 2003. Application of transgenesis in livestock for agriculture and biomedicine. Animal Reproduction Science 79, 291-317. Kues, W.A. und Niemann, H. 2004. The contribution of farm animals to human health. Trends in Biotechnology 22, 286-294. Kues, W.A., Carnwath, J.W., Niemann, H. 2005. From fibroblasts and stem cells: Implications for cell therapies and somatic cloning. Reproduction, Fertility and Development 17, 125-134. Niemann H, Kues W, Carnwath JW. 2005. Transgenic farm animals: present and future. OIE Scientific and Technical Reviews (Rev. Sci. Tech. Off. Int. Epiz.) 24, 284-298. Niemann, H. und Kues, W.A. 2007. Transgenic farm animals – an update. Reproduction, Fertility and Development 19, 762-770. Niemann, H., Carnwath, J.W., Kues, W.A. 2007. Application of array technology to mammalian embryos. Theriogenology 68S, S165-S177. Kues, W.A., Rath, D., Niemann, H. 2008. Reproductive biotechnology goes genomics. CAB Reviews: Perspectives in Agriculture, Veterinary Science, Nutrition and Natural Resources 3, No. 036, 1-18. 5
Page 1 and 2: Aus dem Institut für Nutztiergenet
Page 3: Inhaltsverzeichnis 1.0 Eigene Publi
Page 7 and 8: Bereits 1971 wurden durch Inkubatio
Page 9 and 10: sowie Reproduktion und Krankheitsre
Page 11 and 12: eine verbesserte Allokation und die
Page 13 and 14: 2.5 Transgene Tiere für landwirtsc
Page 15 and 16: Über die homologe Rekombination in
Page 17 and 18: integriert trugen; die Fremd-DNA wa
Page 19 and 20: Abb.5. Konditionale Expressionskont
Page 21 and 22: Belgier und Piedmonteser, sind durc
Page 23 and 24: Abb. 7. Die DNA-Methyltransferase (
Page 25 and 26: 4.0 Ergebnisse und Diskussion Im Er
Page 27 and 28: eseeding of outgrowing cells Tissue
Page 29 and 30: 4.2 Gene knock-down Versuche in tra
Page 31 and 32: vermieden werden. Um zu bestimmen,
Page 33 and 34: starke Expression von hCD59 in Pank
Page 35 and 36: Zellpopulationen als auch in Einzel
Page 37 and 38: ei doppelttransgenen Schweine wurde
Page 39 and 40: 5.0 Zusammenfassung In der vorliege
Page 41 and 42: Chang, H.Y., Chi, J.T., Dudoit, S.,
Page 43 and 44: Golding, M.C., Long, C.R., Carmell,
Page 45 and 46: Kambadur, R., Sharma, M., Smith, T.
Page 47 and 48: Maatouk, D.M., Kellam, L.D., Mann,
Page 49 and 50: Orban, PC, Chui, D, Marth, JD 1992.
Page 51 and 52: Schuelke, M., Wagner, K.R., Stolz,
Page 53 and 54: Yarranton, GT. 1992. Inducible vect
Page 55 and 56:
8.0 Darstellung des eigenen Anteils
Page 57 and 58:
Iqbal, K, Kues, W.A., Niemann, H. 2
Page 59 and 60:
9. Abkürzungsverzeichnis ACTB ß-A
Page 61 and 62:
Abstract Animal Reproduction Scienc
Page 63 and 64:
H. Niemann, W.A. Kues / Animal Repr
Page 65 and 66:
Page 67 and 68:
Page 69 and 70:
Page 71 and 72:
Page 73 and 74:
Page 75 and 76:
Page 77 and 78:
Page 79 and 80:
Page 81 and 82:
Page 83 and 84:
Page 85 and 86:
Page 87:
Page 90 and 91:
246 M. ANGER ET AL. cell cycle in p
Page 92 and 93:
248 M. ANGER ET AL. Statistical Ana
Page 94 and 95:
250 M. ANGER ET AL. Fig. 2. Express
Page 96 and 97:
252 M. ANGER ET AL. Fig. 4. Compara
Page 99 and 100:
Telomere length is reset during ear
Page 101 and 102:
Fig. 2. Telomere length of cloned a
Page 103:
9. Rudolph, K. L., Chang, S., Lee,
Page 106 and 107:
Review TRENDS in Biotechnology Vol.
Page 108 and 109:
Xenotransplantation of porcine orga
Page 110 and 111:
as a novel therapy for liver diseas
Page 112 and 113:
3 Rudolph, N.S. (1999) Biopharmaceu
Page 116 and 117:
BIOLOGY OF REPRODUCTION 72, 1020-10
Page 118 and 119:
1022 KUES ET AL. FIG. 1. Activation
Page 120 and 121:
1024 KUES ET AL. FIG. 3. Induction
Page 122 and 123:
1026 KUES ET AL. FIG. 4. Growth cha
Page 124:
1028 KUES ET AL. 31. Engstrom U, En
Page 136 and 137:
2 P.S. YADAV ET AL. in agriculture
Page 138 and 139:
4 P.S. YADAV ET AL. Fig. 1. Oct4 bu
Page 140 and 141:
6 P.S. YADAV ET AL. individual cell
Page 142 and 143:
8 P.S. YADAV ET AL. TABLE 5. Mainte
Page 155 and 156:
Rev. sci. tech. Off. int. Epiz., 20
Page 157 and 158:
Page 159 and 160:
Page 161 and 162:
Page 163 and 164:
Page 165 and 166:
Page 167 and 168:
Page 169 and 170:
The FASEB Journal Express article f
Page 171 and 172:
To detect CD59 and DAF, immunohisto
Page 173 and 174:
TABLE 1. Expression patterns in ani
Page 175 and 176:
Figure 5. Expression patterns of hD
Page 177 and 178:
demonstrated in transgenic pigs and
Page 179 and 180:
Xenotransplantation 2006: 13: 345-3
Page 181 and 182:
Table 1. Age, sex and transgenic co
Page 183 and 184:
For color development, slides were
Page 185 and 186:
Table 3. Pattern of hCD59 protein e
Page 187 and 188:
thelial cells of small and medium b
Page 189 and 190:
CMV-derived promoter expression can
Page 191 and 192:
Arch Virol (2006) DOI 10.1007/s0070
Page 193 and 194:
Inhibition of PERV expression by RN
Page 195 and 196:
Inhibition of PERV expression by RN
Page 197 and 198:
Parent-of-origin dependent gene-spe
Page 199 and 200:
Fig. 1. (A) Expression of GFP in F1
Page 201 and 202:
An important finding of this study
Page 203 and 204:
CLONING AND STEM CELLS Volume 9, Nu
Page 205 and 206:
366 FIG. 2. Floating spheroids in s
Page 207 and 208:
368 oids and used as donor cells. A
Page 209 and 210:
370 FIG. 4. The three vital piglets
Page 211 and 212:
372 transferase gene knockout pigs.
Page 214 and 215:
CSIRO PUBLISHING Reproduction, Fert
Page 216 and 217:
764 Reproduction, Fertility and Dev
Page 218 and 219:
Page 220 and 221:
Page 222 and 223:
Page 224 and 225:
Abstract Author's personal copy App
Page 226 and 227:
necessary to provide the large numb
Page 228 and 229:
analysis and the information conten
Page 230 and 231:
maternal to embryonic genomic activ
Page 232 and 233:
were determined for individual mous
Page 234 and 235:
References [1] Goodstadt L, Ponting
Page 236 and 237:
inter- and intraplatform reproducib
Page 238 and 239:
2 Perspectives in Agriculture, Vete
Page 240 and 241:
Page 242 and 243:
Page 244 and 245:
Page 246 and 247:
10 Perspectives in Agriculture, Vet
Page 248 and 249:
Page 250 and 251:
Page 252 and 253:
Page 254 and 255:
Page 256 and 257:
integrated in the porcine genome [6
Page 258 and 259:
smaller than 200 nucleotides accord
Page 260 and 261:
normalization (total RNA amount or
Page 262 and 263:
References 1. Cooper DK. Clinical x
Page 264:
mammalian gene expression by small
show all

Experimentelle Untersuchungen zur Transgenese bei Nutztieren

Create successful ePaper yourself

Delete template?

Save as template?