Panel Disdussion

3rd International Congress 3rd International of Nuclear Congress Medicine of Nuclear & 15th Medicine Iranian Annual & 15th Iranian Congress Annual of
Nuclear Congress Medicine of Nuclear Medicine
Shahid Beheshti Shahid Beheshti University University of Medical Sciences of Medical 19-21 Sciences May 201119-21 May 2011
Development of Ho-166 chitosan for radiosynovectomy
Samaneh Zolghadri, Amir Reza Jalilian, Hasan Yousefnia, Ali Bahrami-
Samani, Simindokht Shirvani-Arani, Mohammad Ghannadi-Maragheh
Nuclear Science and Technology Research Institute
Chitosan (CHITO), a natural and biodegradable polysaccharide with
wide range applications in bio-pharmaceutics, agriculture and water
treatment purposes, is an excellent molecule for intra-cavital therapy.
In this research, 166Ho-CHITO complex production is described in
details, followed by determination of complex radiochemical purity,
stability and biodistribution (after intra-articular injection) in wildtype
male rats. Finally a Ho-166 based chitosan kit for ultimate
radiosynovectomy applications in the country was developed.
Production of 166Ho was performed at the Tehran Research Reactor
(TRR) using natHo (n, gamma)166Ho nuclear reaction. 166Ho-
Holmium chitosan complex was prepared and Radiochemical purity
was determined using ITLC. The stability of prepared 166Ho-holmium
chitosan complex was checked up to 48 hours after preparation. The
distribution of injected dose in rat organs up to 144 h after intraarticular
injection of 166Ho-holmium chitosan complex (60 µCi/100ul)
solution was determined for control studies. labeling yield increased
with increasing chitosan concentration and reached above 98% when
the concentration reached 35 mg/3 ml. The highest labeling yield was
achieved at pH=2.8-3.2 while decreased beyond this range. The
labeling yield of 99% was achieved after 30 minutes. The effect of
absence and presence of ascorbic acid (at various concentrations) as
a complex stabilizer were also studied. A kit formulation was
developed for the in-situ preparation of the radiopharmaceutical in
remote clinical centers.
Keywords: chitosan, holmium, biodistribution, labeling
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