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3rd International Congress 3rd International of Nuclear Congress Medicine of Nuclear & 15th Medicine Iranian Annual & 15th Iranian Congress Annual of Nuclear Congress Medicine of Nuclear Medicine Shahid Beheshti Shahid Beheshti University University of Medical Sciences of Medical 19-21 Sciences May 201119-21 May 2011 Evaluation and quality control of Radiolabeled Streptokinase for dignostic purposes Sayyede Fateme Mirazizi, Amir Reza Jalilian, Hossein Sadeghpour Introduction: Cardiovascular diseases are major cause of morbidity and mortality in developing and developed countries. Rapid diagnosis of the thrombosis can be an essential step in management of the stroke people. Methods: Streptokinase was successively labeled with gallium chloride using cyclic DTPA-dianhydrid. In this work a recently developed radiolabeled streptokinase tracer was evaluated in an animal thrombotic model using SPECT imaging and biodictribution studies. Produced [67Ga]-Streptokinase was checked by RTLC, HPLC and SDS-PAGE experiments to check the tracer integrity/purity. The biodistribution studies were performed in femoral vein thrombotic rats (up to 12h) using tissue counting and preliminary SPECT studies respectively (up to 3 h- 48 h). Results: [67Ga]-Streptokinase prepared with suitable radiochemical purity (HPLC >95%, RTLC >99%) was administered to FeCl3 induced thrombotic rats and the percentage of injected dose per gram of tissue (ID/g%) and SPECT images demonstrated the specific binding of the tracer in thrombotic clots located in heart and aortic 2-3 hours post injection. Conclusion: [67Ga]-DTPA-SPK can be a suitable probe for imaging of thrombosis in cardiovascular diseases .Further studies use of Ga- 68 labeled SPK can result in a superior labeled compound due to positron emission properties for PET studies as well as appropriate physical half live. Keywords: Streptokinase, Gallium-67, Thrombosis, SPECT, biodistribution 180
3rd International Congress of of Nuclear Medicine & & 15th 15th Iranian Annual Annual Congress of Congress of Nuclear Medicine Shahid Beheshti Shahid Beheshti University University of Medical of Medical Sciences Sciences 19-21 19-21 May May 2011 Radioprotective agents prevent genotoxicity induced by ionizing radiation in nuclear medicine practice Seyed Jalal Hosseinimehr Department of Radiopharmacy Ionizing radiation passing through living tissues induces damage, these damage are caused directly or indirectly with producing free radicals and toxic agents. Interaction of free radicals with critical macromolecules such as DNA is leading to genotoxicity and then mutation and carcinogenesis. In nuclear medicine practice, personnel and patients expose to ionizing radiation through radiopharmaceutical preparation and administration. Although, gamma irradiation produced by radiopharmaceuticals is not high level, it can induce genotoxicity. There are several compounds were evaluated for protection against genotoxicity induced by external or internal irradiation on culture human lymphocytes in our laboratory. These compounds are mainly natural origin products. Radioprotective effects of Hawthorn, Zataria extracts and hesperidin were investigated against genotoxicity induced by external gamma irradiation in vitro cultured human lymphocytes, they have good radioprotection. In other study, we showed that hesperidin protected significantly, DNA damage induced by gamma irradiation produced by 99mT-MIBI in lymphocytes. These results showed that natural products could be candidate as radioprotective agents in internal and external genotoxicity induced by ionizing radiation. Keywords: Radioprotective, natural product, gamma iradiation, genotoxicity 181
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