Panel Disdussion

3rd International Congress 3rd International of Nuclear Congress Medicine of Nuclear & 15th Medicine Iranian Annual & 15th Iranian Congress Annual of
Nuclear Congress Medicine of Nuclear Medicine
Shahid Beheshti Shahid Beheshti University University of Medical Sciences of Medical 19-21 Sciences May 201119-21 May 2011
Evaluation and quality control of Radiolabeled Streptokinase
for dignostic purposes
Sayyede Fateme Mirazizi, Amir Reza Jalilian, Hossein Sadeghpour
Introduction: Cardiovascular diseases are major cause of morbidity
and mortality in developing and developed countries. Rapid diagnosis
of the thrombosis can be an essential step in management of the
stroke people.
Methods: Streptokinase was successively labeled with gallium
chloride using cyclic DTPA-dianhydrid. In this work a recently
developed radiolabeled streptokinase tracer was evaluated in an
animal thrombotic model using SPECT imaging and biodictribution
studies. Produced [67Ga]-Streptokinase was checked by RTLC, HPLC
and SDS-PAGE experiments to check the tracer integrity/purity. The
biodistribution studies were performed in femoral vein thrombotic
rats (up to 12h) using tissue counting and preliminary SPECT studies
respectively (up to 3 h- 48 h).
Results: [67Ga]-Streptokinase prepared with suitable radiochemical
purity (HPLC >95%, RTLC >99%) was administered to FeCl3 induced
thrombotic rats and the percentage of injected dose per gram of
tissue (ID/g%) and SPECT images demonstrated the specific binding
of the tracer in thrombotic clots located in heart and aortic 2-3 hours
post injection.
Conclusion: [67Ga]-DTPA-SPK can be a suitable probe for imaging
of thrombosis in cardiovascular diseases .Further studies use of Ga-
68 labeled SPK can result in a superior labeled compound due to
positron emission properties for PET studies as well as appropriate
physical half live.
Keywords: Streptokinase, Gallium-67, Thrombosis, SPECT,
biodistribution
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