Tierärztliche Hochschule Hannover Vergleichende Studie zur

Publikation 2
• alveolar oxygen tension (pAO 2 ) was calculated according to (Uystepruyst et al.
2002):
pAO 2 [mmHg] =: (BP - 47) × FIO 2 – (p a CO 2 /RQ), where BP is the barometric
pressure (in mmHg) on the day of the surgery, RQ is the respiratory quotient
determined as 0.80 by (Vermorel et al. 1989) and FIO 2 is the fraction of
inspired oxygen (0.2095 on room air).
• oxygen content in pulmonary capillary blood (CcO 2 ) [mL dL -1 ]: CcO 2 = [Hb] × 1* ×
1,36 + (pAO 2 × 0,003)
(*As the oxygen saturation in capillary blood is assumed to be 100%, the Hb is
multiplied by the factor 1)
• Pulmonary shunt (Qs/Qt) [%]: Qs/Qt = ((CcO 2 - C a O 2 ) / (CcO 2 - C v¯ O 2 )) x 100
Venous blood samples for determination of serum cortisol (Cortisol-Immulite 1000-
Test ® , Siemens Healthcare Diagnostics GmbH, Eschborn, Germany), plasma
epinephrine and norepinephrine (2 CAT RIA, Labor Diagnostika Nord GmbH & Co.
KG, Nordhorn, Germany) from each calf were collected from the jugular vein (via the
catheter) into serum tubes and EDTA-coated tubes, respectively. For cortisol, the
intra-assay coefficient of variation (CV %) was 6.3-10.0% and the inter-assay CV %
was 5.8-8.8%. For both, epinephrine and norepinephrine, intra-assay coefficients of
variation were 4.6% and the inter-assay CV % were 6.1%. Samples were obtained
synchronous to all other measurements and stored at -80°C before analysis.
Data are presented as mean ± standard deviation (SD). A value of P < 0.05 was
considered significant. Normal distribution of data was tested by determining the
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