4 - Central Institute of Brackishwater Aquaculture

7. Infarrnartlon retrieval.
8. Milt replacement program.
9. Milt dlsards procedures.
10. User application programmes.
The decision to preserve a specific population gene pool requires the collection of
baseline information to determine population uniqueness and the traits which
define this uniqueness. Baseline information includes population name, location,
range and distribution, genetic characterlzation, breeding history, life history,
habitat preferences and any specific trait pertaining to the species. Species with no
cryogenic preservation history should be studied to determine a species speclfic
cryogenic processing and storage procedures before gene banking operations can
begin.
The information regarding gene bank users should be based on the population
characterlzation data, stored milt quality and quantity data and the effective
population size represented in the preserved milt. Information on population origin,
breeding history, source habitat parameters, life history traits, genetic analysis and
performance characteristics in culture and natural habitats should be clearly
brought out. This information would help the users to match the preserved stock
with the exlstlng ones and change the management accordingly.
6. Gene Bank Information Database
A database is a collection of data arranged in a systematic order for quick search
and retrieval operation in subsets. The first step involving the designing of*database
is crucial and the essential requirements are: development of data structure based
on the identification of specific data elements. Essential information includes data
needed by the gene bank managers to facilitate decisions on management and
research applications where cryopreserved milt will be used.
The initial data element or trait llst Is developed by answering in complete details a
series of questionnaires as shown In Table-1. The questions are of two types; 1) for
the gene bank operations and 2) the additional information needed to assist user
groups. During the development of Initial tmit Ilst, representatives of all the
potential users lncludlng Government officials should be involved.