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4 - Central Institute of Brackishwater Aquaculture

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Natlonal Workshop-cum-Trainlng on Bioinforrnatica and Information Management in <strong>Aquaculture</strong><br />

Capacity to manage unbalanced data<br />

Ability to create a SAS data set corresponding to any printed table.<br />

8. The inbreeding procedure<br />

The inbreed procedure calculates the covariance or inbreeding coefficients for the<br />

pedigree. PROC INBREED IS unlque In that it handles vary large populations<br />

The inbreed procedures has two modes <strong>of</strong> operation. One mode carries out<br />

analysis on the assumption that all the individuals belong to the same<br />

generation. The other mode dlvides the population Into non-overlapping<br />

generations and analyzes each generation separately assuming that the parents<br />

<strong>of</strong> individuals In the current generation are defined in the prevlous generation.<br />

Proc inbreed also compute average <strong>of</strong> the covariance or Inbreeding coefficient<br />

withln sex categories ~f the gender <strong>of</strong> individuals is known.<br />

9. Strain comparison and heterosis estimation<br />

The diallel cross IS an efficient design for evaluating the performance <strong>of</strong> purebred<br />

and crossbred stocks or lines. In complete diallel cross each stock IS crossed with<br />

itself and all other stocks. For each species that may be stripped artificially the<br />

following mating procedure should be followed to obtain an equal contribution <strong>of</strong><br />

genes from each cross and from each individual within a cross For each stock,<br />

milt from a given number <strong>of</strong> slres IS used to fertilize with an equal number <strong>of</strong><br />

eggs from a given number <strong>of</strong> dams <strong>of</strong> the same and <strong>of</strong> each <strong>of</strong> the other stocks.<br />

Thus both crosses between the pure stocks and all poss~ble cross combinations<br />

between stocks ~ncluding reciprocal are produced<br />

A complete design may provlde estimate <strong>of</strong> additional genetic effect <strong>of</strong> each<br />

stock, overall heterosis, general heterosis for each stock and specific heteros~s<br />

for each cross. Following formula can also be used to determine abovementioned<br />

aspects.<br />

Pn = mean phenotype <strong>of</strong> all purebreds<br />

X ,(, = mean phenotype <strong>of</strong> all crossbreds<br />

Pj/i = mean phenotype <strong>of</strong> one purebred<br />

= Pn+gj/I+gm ]/I (here gm IS the maternal effect)<br />

hl.. = average heterosis for all crosses<br />

=I hIj = X n (n 1) - Pn<br />

hlIj = average heterosis for reciprocal cross<br />

= xi,+X,,-P,-P,)/Z<br />

hlj = average strain heterosis<br />

= EhllJ(n-l)<br />

glj = average indlvldual effect <strong>of</strong> each strain<br />

- Pj + Pn- gm,

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